41 results for "Inoue, Ryuji"

TRPC6 regulates cell cycle progression by modulating membrane potential in bone marrow stromal cells

Ichikawa, Jun; Inoue, Ryuji

Br J Pharmacol · 2014 · English · PubMed Central

BACKGROUND AND PURPOSE: Ca(2+) influx is important for cell cycle progression, but the mechanisms involved seem to vary. We investigated the potential roles of transient receptor potential (TRP) channels and store-operated Ca(2+) entry (SOCE)-related molecules STIM (stromal interaction…

“TRP inflammation” relationship in cardiovascular system

Numata, Tomohiro; Takahashi, Kiriko; Inoue, Ryuji

Semin Immunopathol · 2015 · English · PubMed Central

Despite considerable advances in the research and treatment, the precise relationship between inflammation and cardiovascular (CV) disease remains incompletely understood. Therefore, understanding the immunoinflammatory processes underlying the initiation, progression, and exacerbation of many…

T-channel-like pharmacological properties of highvoltage-activated, nifedipine-insensitive Ca(2+) currents inthe rat terminal mesenteric artery

Morita, Hiromitsu; Shi, Juan; Ito, Yushi et al.

Br J Pharmacol · 2002 · English · PubMed Central

1. Pharmacological properties of nifedipine-insensitive, high voltage-activated Ca(2+) channels in rat mesenteric terminal arteries (NICCs) were investigated and compared with those of α1E and α1G heterologously expressed in BHK and HEK293 cells respectively, using the patch clamp technique. 2…

Endoscopic Biliary Intervention Using Traction Devices for Periampullary Diverticulum

Inoue, Ryuji; Kawakami, Hiroshi; Kubota, Yoshimasa et al.

Intern Med · 2019 · English · PubMed Central

We describe the case a 92-year-old woman who was admitted to our hospital with choledocholithiasis and periampullary diverticulum (PAD). Due to PAD, clear visualization of the ampulla of Vater could not be obtained. Although selective bile duct cannulation was difficult, a 7-Fr plastic stent was…

Cellular mechanism for herbal medicine Junchoto to facilitate intestinal Cl(−)/water secretion that involves cAMP-dependent activation of CFTR

Numata, Tomohiro; Sato-Numata, Kaori; Okada, Yasunobu et al.

J Nat Med · 2018 · English · PubMed Central

Constipation is a common symptom frequently compromising the quality of daily life. Several mechanistically different drugs have been used to mitigate constipation, including Japanese herbal (Kampo) medicines. However, the mechanisms of their actions are often not well understood. Here we aimed to…

Multiple regulation by external ATP of nifedipine-insensitive, high voltage-activated Ca(2+) current in guinea-pig mesenteric terminal arteriole

Morita, Hiromitsu; Sharada, Thapaliya; Takewaki, Tadashi et al.

J Physiol · 2002 · English · PubMed Central

We investigated the receptor-mediated regulation of nifedipine-insensitive, high voltage-activated Ca(2+) currents in guinea-pig terminal mesenteric arterioles (I(mVDCC)) using the whole-cell clamp technique. Screening of various vasoactive substances revealed that ATP, histamine and substance P…

The Role of TRPM4 in Cardiac Electrophysiology and Arrhythmogenesis

Hu, Yaopeng; Cang, Jiehui; Hiraishi, Keizo et al.

Int J Mol Sci · 2023 · English · PubMed Central

The transient receptor potential melastatin 4 (TRPM4) channel is a non-selective cation channel that activates in response to increased intracellular Ca(2+) levels but does not allow Ca(2+) to pass through directly. It plays a crucial role in regulating diverse cellular functions associated with…

A self-limiting regulation of vasoconstrictor-activated TRPC3/C6/C7 channels coupled to PI(4,5)P(2)-diacylglycerol signalling

Imai, Yuko; Itsuki, Kyohei; Okamura, Yasushi et al.

J Physiol · 2011 · English · PubMed Central

Activation of transient receptor potential (TRP) canonical TRPC3/C6/C7 channels by diacylglycerol (DAG) upon stimulation of phospholipase C (PLC)-coupled receptors results in the breakdown of phosphoinositides (PIPs). The critical importance of PIPs to various ion-transporting molecules is well…

Cyclic GMP-dependent but G-kinase-independent inhibition of Ca(2+)-dependent Cl(−) currents by NO donors in cat tracheal smooth muscle

Waniishi, Yoshiki; Inoue, Ryuji; Morita, Hiromitsu et al.

J Physiol · 1998 · English · PubMed Central

1. The effects of NO donors on Ca(2+)-dependent Cl(−) currents (I(Cl(Ca))) were investigated in freshly isolated cat tracheal myocytes using the whole-cell patch clamp technique. 2. With nystatin-perforated whole-cell recording, carbachol (CCh, ≥ 1 μm) induced a transient inward current (I((CCh)))…

Distinct contributions of LRRC8A and its paralogs to the VSOR anion channel from those of the ASOR anion channel

Sato-Numata, Kaori; Numata, Tomohiro; Inoue, Ryuji et al.

Channels (Austin) · 2016 · English · PubMed Central

Volume- and acid-sensitive outwardly rectifying anion channels (VSOR and ASOR) activated by swelling and acidification exhibit voltage-dependent inactivation and activation time courses, respectively. Recently, LRRC8A and some paralogs were shown to be essentially involved in the activity and…

Theoretical Investigation of the Mechanism by which A Gain-of-Function Mutation of the TRPM4 Channel Causes Conduction Block

Hu, Yaopeng; Li, Qin; Shen, Yanghua et al.

Int J Mol Sci · 2021 · English · PubMed Central

In the heart, TRPM4 is most abundantly distributed in the conduction system. Previously, a single mutation, ‘E7K’, was identified in its distal N-terminus to cause conduction disorder because of enhanced cell-surface expression. It remains, however, unclear how this expression increase leads to…

PLC-mediated PI(4,5)P(2) hydrolysis regulates activation and inactivation of TRPC6/7 channels

Itsuki, Kyohei; Imai, Yuko; Hase, Hideharu et al.

J Gen Physiol · 2014 · English · PubMed Central

Transient receptor potential classical (or canonical) (TRPC)3, TRPC6, and TRPC7 are a subfamily of TRPC channels activated by diacylglycerol (DAG) produced through the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PI(4,5)P(2)) by phospholipase C (PLC). PI(4,5)P(2) depletion by a…

Voltage-sensing phosphatase reveals temporal regulation of TRPC3/C6/C7 channels by membrane phosphoinositides

Itsuki, Kyohei; Imai, Yuko; Okamura, Yasushi et al.

Channels (Austin) · 2012 · English · PubMed Central

TRPC3/C6/C7 channels, a subgroup of classical/canonical TRP channels, are activated by diacylglycerol produced via activation of phospholipase C (PLC)-coupled receptors. Recognition of the physiological importance of these channels has been steadily growing, but the mechanism by which they are…

GMP-mediated regulation of cardiovascular Ca(2+ )entry channel TRPC6 and its pathophysiological implications

Inoue, Ryuji; Jian, Zhong; Lin, Hai et al.

BMC Pharmacol · 2009 · English · PubMed Central

Multiple regulation by calcium of murine homologues of transient receptor potential proteins TRPC6 and TRPC7 expressed in HEK293 cells

Shi, Juan; Mori, Emiko; Mori, Yasuo et al.

J Physiol · 2004 · English · PubMed Central

We investigated, by using the patch clamp technique, Ca(2+)-mediated regulation of heterologously expressed TRPC6 and TRPC7 proteins in HEK293 cells, two closely related homologues of the transient receptor potential (TRP) family and molecular candidates for native receptor-operated Ca(2+) entry…

Critical Role of TRPC1-Mediated Ca(2+) Entry in Decidualization of Human Endometrial Stromal Cells

Kawarabayashi, Yasuhiro; Hai, Lin; Honda, Akira et al.

Mol Endocrinol · 2012 · English · PubMed Central

Decidualization is an ovarian steroid-induced remodeling/differentiation process of uterus essential for embryo implantation and placentation. Here, we investigated the possible involvement of enhanced Ca(2+) dynamics in the decidualization process in human endometrial stromal cells (hESC) in its…

Dynamics of receptor-operated Ca(2+) currents through TRPC channels controlled via the PI(4,5)P(2)-PLC signaling pathway

Mori, Masayuki X.; Itsuki, Kyohei; Hase, Hideharu et al.

Front Pharmacol · 2015 · English · PubMed Central

Transient receptor potential canonical (TRPC) channels are Ca(2+)-permeable, nonselective cation channels that carry receptor-operated Ca(2+) currents (ROCs) triggered by receptor-induced, phospholipase C (PLC)-catalyzed hydrolysis of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P(2)]. Within the…

Phosphorylation of TRPC6 Channels at Thr(69) Is Required for Anti-hypertrophic Effects of Phosphodiesterase 5 Inhibition

Nishida, Motohiro; Watanabe, Kenta; Sato, Yoji et al.

J Biol Chem · 2010 · English · PubMed Central

Activation of Ca(2+) signaling induced by receptor stimulation and mechanical stress plays a critical role in the development of cardiac hypertrophy. A canonical transient receptor potential protein subfamily member, TRPC6, which is activated by diacylglycerol and mechanical stretch, works as an…

Construction of a Fluorescent Screening System of Allosteric Modulators for the GABA(A) Receptor Using a Turn-On Probe

Sakamoto, Seiji; Yamaura, Kei; Numata, Tomohiro et al.

ACS Cent Sci · 2019 · English · PubMed Central

[Image: see text] γ-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. The fast inhibitory actions of GABA are mainly mediated by GABA(A) receptors (GABA(A)Rs), which are widely recognized as clinically relevant drug targets. However, it remains…

TRPM7‐mediated spontaneous Ca(2+) entry regulates the proliferation and differentiation of human leukemia cell line K562

Takahashi, Kiriko; Umebayashi, Chisato; Numata, Tomohiro et al.

Physiol Rep · 2018 · English · PubMed Central

Continuous Ca(2+) influx is essential to maintain intracellular Ca(2+) homeostasis and its dysregulation leads to a variety of cellular dysfunctions. In this study, we explored the functional roles of spontaneous Ca(2+) influx for the proliferation and differentiation of a human erythromyeloid…