19 hasil untuk "Li, Hai-Ri"
Menampilkan 1–19 · 0,59 detik · RSS
-
Artikel Jurnal Teks lengkap
RNA-Seq Analysis of Gene Expression and Alternative Splicing by Double-Random Priming Strategy
…RNA-seq approach capable of generating strand-specific information [Li et al., Proc Natl Acad Sci USA 105:20179–20184, 2008]. Poly(A)(+) RNA from a treated and an untreated sample were utilized to generate RNA-seq libraries that were sequenced on the Illumina GA1 analyzer. Statistical analysis of…
-
Artikel Jurnal Teks lengkap
Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq strategy to profile poly(A+) RNA
We summarize 12 experimental methods that have been developed for profiling gene expression by focusing on the 3′-end of poly(A+) mRNA, distilling both common and unique features. Of this family of methods, we provide detailed protocol for MAPS, a method we believe is the simplest and most…
-
Artikel Jurnal Teks lengkap
Chronic adolescent exposure to cannabis in mice leads to sex-biased changes in gene expression networks across brain regions
During adolescence, frequent and heavy cannabis use can lead to serious adverse health effects and cannabis use disorder (CUD). Rodent models of adolescent exposure to the main psychoactive component of cannabis, delta-9-tetrahydrocannabinol (THC), mimic the behavioral alterations observed in…
-
Artikel Jurnal Teks lengkap
Profiling alternatively spliced mRNA isoforms for prostate cancer classification
BACKGROUND: Prostate cancer is one of the leading causes of cancer illness and death among men in the United States and world wide. There is an urgent need to discover good biomarkers for early clinical diagnosis and treatment. Previously, we developed an exon-junction microarray-based assay and…
Juga tersedia di: DOAJ
-
Artikel Jurnal Teks lengkap
cFLIP expression is altered in severe corticosteroid-resistant asthma
Dysregulation of alternative splicing of mRNA precursors is known to contribute to numerous human diseases. In this study we carried out the first systematic search for asthma-associated changes in alternative splicing events, using a model of Aspergillus fumigatus (A. fumigatus)-sensitized mice…
-
Artikel Jurnal Teks lengkap
MAASE: An alternative splicing database designed for supporting splicing microarray applications
Alternative splicing is a prominent feature of higher eukaryotes. Understanding of the function of mRNA isoforms and the regulation of alternative splicing is a major challenge in the post-genomic era. The development of mRNA isoform sensitive microarrays, which requires precise splice-junction…
-
Artikel Jurnal Teks lengkap
Preprocessing and Quality Control Strategies for Illumina DASL Assay-Based Brain Gene Expression Studies with Semi-Degraded Samples
Available statistical preprocessing or quality control analysis tools for gene expression microarray datasets are known to greatly affect downstream data analysis, especially when degraded samples, unique tissue samples, or novel expression assays are used. It is therefore important to assess the…
-
Artikel Jurnal Teks lengkap
Genome-wide expression assay comparison across frozen and fixed postmortem brain tissue samples
BACKGROUND: Gene expression assays have been shown to yield high quality genome-wide data from partially degraded RNA samples. However, these methods have not yet been applied to postmortem human brain tissue, despite their potential to overcome poor RNA quality and other technical limitations…
Juga tersedia di: DOAJ
-
Artikel Jurnal Teks lengkap
Efficient Generation of Human iPS Cells by a Synthetic Self-Replicative RNA
The generation of human induced Pluripotent Stem (iPS) cells holds great promise for development of regenerative medicine therapies to treat a wide range of human diseases. However, the generation of iPS cells in the absence of integrative DNA vectors remains problematic. Here we report a simple…
-
Artikel Jurnal Teks lengkap
An evolutionarily conserved DNA architecture determines target specificity of the TWIST family bHLH transcription factors
Basic helix–loop–helix (bHLH) transcription factors recognize the canonical E-box (CANNTG) to regulate gene transcription; however, given the prevalence of E-boxes in a genome, it has been puzzling how individual bHLH proteins selectively recognize E-box sequences on their targets. TWIST is a bHLH…
-
Artikel Jurnal Teks lengkap
Thyroid Hormone Receptor Repression Linked to Type I Pneumocyte Associated Respiratory Distress Syndrome
Although the lung is a defining feature of air-breathing animals, the pathway controlling the formation of the type I pneumocyte, the cell that mediates gas exchange, is poorly understood. In contrast, glucocorticoids and their cognate receptor (GR) have long been known to promote type II…
-
Artikel Jurnal Teks lengkap
Age-Dependent Brain Gene Expression and Copy Number Anomalies in Autism Suggest Distinct Pathological Processes at Young Versus Mature Ages
Autism is a highly heritable neurodevelopmental disorder, yet the genetic underpinnings of the disorder are largely unknown. Aberrant brain overgrowth is a well-replicated observation in the autism literature; but association, linkage, and expression studies have not identified genetic factors that…
-
Artikel Jurnal Teks lengkap
Single-nucleus genomics in outbred rats with divergent cocaine addiction-like behaviors reveals changes in amygdala GABAergic inhibition
The amygdala processes positive and negative valence and contributes to addiction, but the cell-type-specific gene regulatory programs involved are unknown. We generated an atlas of single-nucleus gene expression and chromatin accessibility in the amygdala of outbred rats with high and low cocaine…
-
Artikel Jurnal Teks lengkap
A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using…
-
Artikel Jurnal Teks lengkap
MiR-215 is induced post-transcriptionally via HIF-Drosha complex and mediates glioma-initiating cell adaptation to hypoxia by targeting KDM1B
The hypoxic tumor microenvironment serves as a niche for maintaining the glioma-initiating cells (GICs) that are critical for glioblastoma (GBM) occurrence and recurrence. Here we report that hypoxia-induced miR-215 is vital for reprograming GICs to fit the hypoxic microenvironment via suppressing…
-
Artikel Jurnal Teks lengkap
Targeted degradation of sense and antisense C9orf72 RNA foci as therapy for ALS and frontotemporal degeneration
Expanded hexanucleotide repeats in the chromosome 9 open reading frame 72 (C9orf72) gene are the most common genetic cause of ALS and frontotemporal degeneration (FTD). Here, we identify nuclear RNA foci containing the hexanucleotide expansion (GGGGCC) in patient cells, including white blood cells…