29 results for "Li-Fu Li"
Showing 1–20 · 1.26 seconds · RSS
-
Journal Article Full text
Invertase SUC2 Is the Key Hydrolase for Inulin Degradation in Saccharomyces cerevisiae
Specific Saccharomyces cerevisiae strains were recently found to be capable of efficiently utilizing inulin, but genetic mechanisms of inulin hydrolysis in yeast remain unknown. Here we report functional characteristics of invertase SUC2 from strain JZ1C and demonstrate that SUC2 is the key enzyme…
-
Journal Article Full text
Engineering a natural Saccharomyces cerevisiae strain for ethanol production from inulin by consolidated bioprocessing
BACKGROUND: The yeast Saccharomyces cerevisiae is an important eukaryotic workhorse in traditional and modern biotechnology. At present, only a few S. cerevisiae strains have been extensively used as engineering hosts. Recently, an astonishing genotypic and phenotypic diversity of S. cerevisiae was…
-
Journal Article Full text
Enhanced electromechanical coupling of a nanomechanical resonator to coupled superconducting cavities
We investigate the electromechanical coupling between a nanomechanical resonator and two parametrically coupled superconducting coplanar waveguide cavities that are driven by a two-mode squeezed microwave source. We show that, with the selective coupling of the resonator to the cavity Bogoliubov…
-
Journal Article Full text
Direct bioconversion of brown algae into ethanol by thermophilic bacterium Defluviitalea phaphyphila
BACKGROUND: Brown algae are promising feedstocks for biofuel production with inherent advantages of no structural lignin, high growth rate, and no competition for land and fresh water. However, it is difficult for one microorganism to convert all components of brown algae with different…
-
Journal Article Full text
Defluviitalea phaphyphila sp. nov., a Novel Thermophilic Bacterium That Degrades Brown Algae
Brown algae are one of the largest groups of oceanic primary producers for CO(2) removal and carbon sinks for coastal regions. However, the mechanism for brown alga assimilation remains largely unknown in thermophilic microorganisms. In this work, a thermophilic alginolytic community was enriched…
-
Journal Article Full text
Preparing entangled states between two NV centers via the damping of nanomechanical resonators
We propose an efficient scheme for preparing entangled states between two separated nitrogen-vacancy (NV) centers in a spin-mechanical system via a dissipative quantum dynamical process. The proposal actively exploits the nanomechanical resonator (NAMR) damping to drive the NV centers to the target…
-
Journal Article Full text
Spontaneous large-scale autolysis in Clostridium acetobutylicum contributes to generation of more spores
Autolysis is a widespread phenomenon in bacteria. In batch fermentation of Clostridium acetobutylicum ATCC 824, there is a spontaneous large-scale autolysis phenomenon with significant decrease of cell density immediately after exponential phase. To unravel the role of autolysis, an…
-
Journal Article Full text
Exploration of Two Pectate Lyases from Caldicellulosiruptor bescii Reveals that the CBM66 Module Has a Crucial Role in Pectic Biomass Degradation
Pectin deconstruction is the initial step in breaking the recalcitrance of plant biomass by using selected microorganisms that encode pectinolytic enzymes. Pectate lyases that cleave the α-1,4-galacturonosidic linkage of pectin are widely used in industries such as papermaking and fruit softening…
-
Journal Article Full text
Naphthylacetic Acid and Tea Polyphenol Application Promote Biomass and Lipid Production of Nervonic Acid-Producing Microalgae
Mychonastes afer HSO-3-1 is a potential producer of nervonic acid, which could be accumulated to 2–3% of dry cell weight. Improving the productivity of nervonic acid is critical to promote the commercialization of this product. In this study, 1-naphthylacetic acid (NAA) and tea polyphenol (TP) were…
-
Journal Article Full text
Synergistic Cellulose Hydrolysis Dominated by a Multi-Modular Processive Endoglucanase from Clostridium cellulosi
Recalcitrance of biomass feedstock remains a challenge for microbial conversion of lignocellulose into biofuel and biochemicals. Clostridium cellulosi, one thermophilic bacterial strain dominated in compost, could hydrolyze lignocellulose at elevated temperature by secreting more than 38 glycoside…
-
Journal Article Full text
A Rapid Method for the Determination of Fucoxanthin in Diatom
Fucoxanthin is a natural pigment found in microalgae, especially diatoms and Chrysophyta. Recently, it has been shown to have anti-inflammatory, anti-tumor, and anti-obesityactivity in humans. Phaeodactylum tricornutum is a diatom with high economic potential due to its high content of fucoxanthin…
-
Journal Article Full text
Rapid Sorting of Fucoxanthin-Producing Phaeodactylum tricornutum Mutants by Flow Cytometry
Fucoxanthin, which is widely found in seaweeds and diatoms, has many benefits to human health, such as anti-diabetes, anti-obesity, and anti-inflammatory physiological activities. However, the low content of fucoxanthin in brown algae and diatoms limits the commercialization of this product. In…
-
Journal Article Full text
Overexpression of plastid lipid-associated protein in marine diatom enhances the xanthophyll synthesis and storage
Plastoglobules, which are lipoprotein structures surrounded by a single hydrophobic phospholipid membrane, are subcellular organelles in plant chromoplasts and chloroplasts. They contain neutral lipids, tocopherols, quinones, chlorophyll metabolites, carotenoids and their derivatives. Proteomic…
-
Journal Article Full text
Two Distinct α-l-Arabinofuranosidases in Caldicellulosiruptor Species Drive Degradation of Arabinose-Based Polysaccharides
Species in the extremely thermophilic genus Caldicellulosiruptor can degrade unpretreated plant biomass through the action of multimodular glycoside hydrolases. To date, most focus with these bacteria has been on hydrolysis of glucans and xylans, while the biodegradation mechanism for…
-
Journal Article Full text
Distinct Roles for Carbohydrate-Binding Modules of Glycoside Hydrolase 10 (GH10) and GH11 Xylanases from Caldicellulosiruptor sp. Strain F32 in Thermostability and Catalytic Efficiency
Xylanases are crucial for lignocellulosic biomass deconstruction and generally contain noncatalytic carbohydrate-binding modules (CBMs) accessing recalcitrant polymers. Understanding how multimodular enzymes assemble can benefit protein engineering by aiming at accommodating various environmental…
-
Journal Article Full text
Modulation of the Acetone/Butanol Ratio during Fermentation of Corn Stover-Derived Hydrolysate by Clostridium beijerinckii Strain NCIMB 8052
Producing biobutanol from lignocellulosic biomass has shown promise to ultimately reduce greenhouse gases and alleviate the global energy crisis. However, because of the recalcitrance of a lignocellulosic biomass, a pretreatment of the substrate is needed which in many cases releases soluble lignin…
-
Journal Article Full text
Inducible promoters of bacterial microcompartments improve the CRISPR/Cas9 tools for efficient metabolic engineering of Clostridium ljungdahlii
Clostridium ljungdahlii, as a model acetogen strain, represents a novel platform for biotechnological production for CO(2) fixation. The genome of C. ljungdahlii harbors two gene loci associated with glycyl radical enzyme-associated microcompartments (GRMs), which are predicted to play essential…
-
Journal Article Full text
The phylogeny of the Anderson's White‐bellied Rat (Niviventer andersoni) based on complete mitochondrial genomes
The phylogenetic structure of the genus Niviventer has been studied based on several individual mitochondrial and nuclear genes, but the results seem to be inconsistent. In order to clarify the phylogeny of Niviventer, we sequenced the complete mitochondrial genome of white‐bellied rat (Niviventer…
-
Journal Article Full text
Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide
Bioethanol production from syngas using acetogenic bacteria has attracted considerable attention in recent years. However, low ethanol yield is the biggest challenge that prevents the commercialization of syngas fermentation into biofuels using microbial catalysts. The present study demonstrated…