16 hasil untuk "Muhammad Ashfaq"
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DNA Barcode Analysis of Thrips (Thysanoptera) Diversity in Pakistan Reveals Cryptic Species Complexes
Although thrips are globally important crop pests and vectors of viral disease, species identifications are difficult because of their small size and inconspicuous morphological differences. Sequence variation in the mitochondrial COI-5ʹ (DNA barcode) region has proven effective for the…
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The first initiative of DNA barcoding of ornamental plants from Egypt and potential applications in horticulture industry
DNA barcoding relies on short and standardized gene regions to identify species. The agricultural and horticultural applications of barcoding such as for marketplace regulation and copyright protection remain poorly explored. This study examines the effectiveness of the standard plant barcode…
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Mapping global biodiversity connections with DNA barcodes: Lepidoptera of Pakistan
Sequences from the DNA barcode region of the mitochondrial COI gene are an effective tool for specimen identification and for the discovery of new species. The Barcode of Life Data Systems (BOLD) (www.boldsystems.org) currently hosts 4.5 million records from animals which have been assigned to more…
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Use of Recombinant Tobacco Mosaic Virus To Achieve RNA Interference in Plants against the Citrus Mealybug, Planococcus citri (Hemiptera: Pseudococcidae)
The citrus mealybug, Planococcus citri, is an important plant pest with a very broad plant host range. P. citri is a phloem feeder and loss of plant vigor and stunting are characteristic symptoms induced on a range of host plants, but P. citri also reduces fruit quality and causes fruit drop…
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BIN overlap confirms transcontinental distribution of pest aphids (Hemiptera: Aphididae)
DNA barcoding is highly effective for identifying specimens once a reference sequence library is available for the species assemblage targeted for analysis. Despite the great need for an improved capacity to identify the insect pests of crops, the use of DNA barcoding is constrained by the lack of…
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Analyzing Mosquito (Diptera: Culicidae) Diversity in Pakistan by DNA Barcoding
Background: Although they are important disease vectors mosquito biodiversity in Pakistan is poorly known. Recent epidemics of dengue fever have revealed the need for more detailed understanding of the diversity and distributions of mosquito species in this region. DNA barcoding improves the…
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DNA Barcoding of Bemisia tabaci Complex (Hemiptera: Aleyrodidae) Reveals Southerly Expansion of the Dominant Whitefly Species on Cotton in Pakistan
Background: Although whiteflies (Bemisia tabaci complex) are an important pest of cotton in Pakistan, its taxonomic diversity is poorly understood. As DNA barcoding is an effective tool for resolving species complexes and analyzing species distributions, we used this approach to analyze genetic…
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Evaluation of Sulfadiazine Degradation in Three Newly Isolated Pure Bacterial Cultures
This study is aimed to assess the biodegradation of sulfadiazine (SDZ) and characterization of heavy metal resistance in three pure bacterial cultures and also their chemotactic response towards 2-aminopyrimidine. The bacterial cultures were isolated from pig manure, activated sludge and sediment…
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Assembling a DNA barcode reference library for the spiders (Arachnida: Araneae) of Pakistan
Morphological study of 1,795 spiders from sites across Pakistan placed these specimens in 27 families and 202 putative species. COI sequences >400 bp recovered from 1,782 specimens were analyzed using neighbor-joining trees, Bayesian inference, barcode gap, and Barcode Index Numbers (BINs)…
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Genome-wide identification and functional characterization of NPR1-like genes in Actinidia deliciosa
NPR1 (Nonexpresser of Pathogenesis-Related Genes 1) is a central regulator of salicylic acid (SA)-mediated defense signaling in plants and plays a pivotal role in modulating systemic acquired resistance (SAR). Despite its functional importance in biotic stress responses, a comprehensive…
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Foliar application of mepiquat chloride and nitrogen improves yield and fiber quality traits of cotton ( Gossypium hirsutum L.)
Cotton (Gossypium hirsutum L.) is one of the most important cash crops primarily grown for fiber. It is a perennial crop with indeterminate growth pattern. Nitrogen (N) is extremely important for vegetative growth as balanced N-nutrition improves photosynthesis, resulting in better vegetative…
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Optimizing sowing date for peanut genotypes in arid and semi-arid subtropical regions
Peanut (Arachis hypogea L.) is an important nut crop extensively grown in rainfed regions of Pakistan. The crop requires low inputs; thus, could grow successfully under diverse environmental conditions. Due to pegging ability, peanut grows aggressively in sandy and sandy-loam soils. However, it has…
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Insect diversity in the Saharo-Arabian region: Revealing a little-studied fauna by DNA barcoding
Although insects dominate the terrestrial fauna, sampling constraints and the poor taxonomic knowledge of many groups have limited assessments of their diversity. Passive sampling techniques and DNA-based species assignments now make it possible to overcome these barriers. For example, Malaise…
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Foliar application of potassium and moringa leaf extract improves growth, physiology and productivity of kabuli chickpea grown under varying sowing regimes
Chickpea (Cicer arietinum L.) is of prime importance because of vital source of protein as major food legume. Globally, it is cultivated on large area to meet dietary requirements of humans. Climatic extremes (erratic rainfall, extreme high and low temperature) are key restrains for its production…
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Epidemiological studies of sleep disorder in educational community of Pakistani population, its major risk factors and associated diseases
Sleep is one of the most important functions of the life. The disturbance in sleep or quality of sleep leads to several dysfunctions of the human body. This study aimed to investigate the prevalence of sleep disorders, their possible risk factors and their association with other health problems…
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Combined application of zinc and silicon alleviates terminal drought stress in wheat by triggering morpho-physiological and antioxidants defense mechanisms
Wheat is an important global staple food crop; however, its productivity is severely hampered by changing climate. Erratic rain patterns cause terminal drought stress, which affect reproductive development and crop yield. This study investigates the potential and zinc (Zn) and silicon (Si) to…