32 results for "Schulz, Daniel"

Two-Stage Pedestrian Detection Model Using a New Classification Head for Domain Generalization

Schulz, Daniel; Perez, Claudio A.

Sensors (Basel) · 2023 · English · PubMed Central

Pedestrian detection based on deep learning methods have reached great success in the past few years with several possible real-world applications including autonomous driving, robotic navigation, and video surveillance. In this work, a new neural network two-stage pedestrian detector with a new…

Disentangling Puzzles of Spatial Scales and Participation in Environmental Governance—The Case of Governance Re-scaling Through the European Water Framework Directive

Newig, Jens; Schulz, Daniel; Jager, Nicolas W.

Environ Manage · 2016 · English · PubMed Central

This article attempts to shed new light on prevailing puzzles of spatial scales in multi-level, participatory governance as regards the democratic legitimacy and environmental effectiveness of governance systems. We focus on the governance re-scaling by the European Water Framework Directive, which…

Rpb4 Subunit Functions Mainly in mRNA Synthesis by RNA Polymerase II

Schulz, Daniel; Pirkl, Nicole; Lehmann, Elisabeth et al.

J Biol Chem · 2014 · English · PubMed Central

RNA polymerase II (Pol II) is the central enzyme that carries out eukaryotic mRNA transcription and consists of a 10-subunit catalytic core and a subcomplex of subunits Rpb4 and Rpb7 (Rpb4/7). Rpb4/7 has been proposed to dissociate from Pol II, enter the cytoplasm, and function there in mRNA…

Informed Machine Learning

Schulz, Daniel; Bauckhage, Christian

Springer Nature · 2025 · English · DOAB

This open access book presents the concept of Informed Machine Learning and demonstrates its practical use with a compelling collection of applications of this paradigm in industrial and business use cases. These range from health care over manufacturing and material science to more advanced…

Also available at: OAPEN

In-Depth Characterization of Monocyte-Derived Macrophages using a Mass Cytometry-Based Phagocytosis Assay

Schulz, Daniel; Severin, Yannik; Zanotelli, Vito Riccardo Tomaso et al.

Sci Rep · 2019 · English · PubMed Central

Phagocytosis is a process in which target cells or particles are engulfed and taken up by other cells, typically professional phagocytes; this process is crucial in many physiological processes and disease states. The detection of targets for phagocytosis is directed by a complex repertoire of cell…

Inactivation of the Mouse L-Proline Transporter PROT Alters Glutamatergic Synapse Biochemistry and Perturbs Behaviors Required to Respond to Environmental Changes

Schulz, Daniel; Morschel, Julia; Schuster, Stefanie et al.

Front Mol Neurosci · 2018 · English · PubMed Central

The endogenous neutral amino acid L-proline exhibits a variety of physiological and behavioral actions in the nervous system, highlighting the importance of accurately regulating its extracellular abundance. The L-proline transporter PROT (Slc6A7) is believed to control the spatial and temporal…

Optimizing multiplexed imaging experimental design through tissue spatial segregation estimation

Bost, Pierre; Schulz, Daniel; Engler, Stefanie et al.

Nat Methods · 2022 · English · PubMed Central

Recent advances in multiplexed imaging methods allow simultaneous detection of dozens of proteins and hundreds of RNAs, enabling deep spatial characterization of both healthy and diseased tissues. Parameters for the design of optimal multiplex imaging studies, especially those estimating how much…

Misfolded membrane proteins are specifically recognized by the transmembrane domain of the Hrd1p ubiquitin ligase

Sato, Brian K.; Schulz, Daniel; Do, Phong H. et al.

Mol Cell · 2009 · English · PubMed Central

Quality control pathways such as ER-associated degradation (ERAD) employ a small number of factors to specifically recognize a wide variety of protein substrates. Delineating the mechanisms of substrate selection is a principle goal in studying quality control. The Hrd1p ubiquitin ligase mediates…

Chapter 13 Differentiation and the European Central Bank

Schulz, Daniel F.; Verdun, Amy

Taylor & Francis · 2022 · English · DOAB

As the guardian of the euro, the European Central Bank (ECB) oversees a prime example of differentiated integration. Against the backdrop of the multiple crises of the euro’s second decade, this contribution asks how the ECB has dealt with differentiation. It analyses both the historical…

Also available at: OAPEN

Comparative dynamic transcriptome analysis (cDTA) reveals mutual feedback between mRNA synthesis and degradation

Sun, Mai; Schwalb, Björn; Schulz, Daniel et al.

Genome Res · 2012 · English · PubMed Central

To monitor eukaryotic mRNA metabolism, we developed comparative dynamic transcriptome analysis (cDTA). cDTA provides absolute rates of mRNA synthesis and decay in Saccharomyces cerevisiae (Sc) cells with the use of Schizosaccharomyces pombe (Sp) as an internal standard. cDTA uses nonperturbing…

Simultaneous Multiplexed Imaging of mRNA and Proteins with Subcellular Resolution in Breast Cancer Tissue Samples by Mass Cytometry

Schulz, Daniel; Zanotelli, Vito Riccardo Tomaso; Fischer, Jana Raja et al.

Cell Syst · 2018 · English · PubMed Central

To build comprehensive models of cellular states and interactions in normal and diseased tissue, genetic and proteomic information must be extracted with single-cell and spatial resolution. Here, we extended imaging mass cytometry to enable multiplexed detection of mRNA and proteins in tissues…

Also available at: PubMed Central

In vitro and in vivo derived macrophages occupy distinct phenotypic states

Chevrier, Stéphane; Zanotelli, Vito R. T.; Schulz, Daniel et al.

bioRxiv · 2025 · English · PubMed Central

Monocyte-derived macrophages (MDMs) are widely used to model human macrophage biology in vitro and standardized polarization conditions were proposed to recapitulate the different macrophage activation states. Although surface markers specific for distinct MDM populations have been identified, a…

miCAT: A toolbox for analysis of cell phenotypes and interactions in multiplex image cytometry data

Schapiro, Denis; Jackson, Hartland W; Raghuraman, Swetha et al.

Nat Methods · 2017 · English · PubMed Central

Single-cell, spatially resolved ‘omics analysis of tissues is poised to transform biomedical research and clinical practice. We have developed an open-source, computational multiplex image cytometry analysis toolbox (miCAT) to enable interactive, quantitative, and comprehensive exploration of…

Pierre Rosanvallon's Political Thought

Flügel-Martinsen, Oliver; Martinsen, Franziska; Sawyer, Stephen W. et al.

Bielefeld University Press · 2018 · English · DOAB

The work of Pierre Rosanvallon has increasingly found itself at the center of debates in democratic and political theory – although only few of his numerous monographs have thus far been translated from French. This interdisciplinary volume, the first comprehensive collection on his political…

Also available at: OAPEN

Dynamic transcriptome analysis measures rates of mRNA synthesis and decay in yeast

Miller, Christian; Schwalb, Björn; Maier, Kerstin et al.

Mol Syst Biol · 2011 · English · PubMed Central

To obtain rates of mRNA synthesis and decay in yeast, we established dynamic transcriptome analysis (DTA). DTA combines non-perturbing metabolic RNA labeling with dynamic kinetic modeling. DTA reveals that most mRNA synthesis rates are around several transcripts per cell and cell cycle, and most…

Memory CD8(+) T cells colocalize with IL-7(+) stromal cells in bone marrow and rest in terms of proliferation and transcription

Sercan Alp, Özen; Durlanik, Sibel; Schulz, Daniel et al.

Eur J Immunol · 2015 · English · PubMed Central

It is believed that memory CD8(+) T cells are maintained in secondary lymphoid tissues, peripheral tissues, and BM by homeostatic proliferation. Their survival has been shown to be dependent on IL-7, but it is unclear where they acquire it. Here we show that in murine BM, memory CD8(+) T cells…