61 hasil untuk "Burger, Michael"
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Artikel Jurnal
Rezension: Mondbeglänzte Zaubernächte. Das Kino von Tim Burton von Christian Heger
Christian Heger erkundet in seiner Monografie das gesamte künstlerische Schaffen von Tim Burton in einer biografisch-chronologischen Darstellung, die aus filmtheoretischer Sicht nicht durchwegs überzeugt.
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Recovering From the Recovery Narrative: On Globalism, Green Jobs and Cyborg Civilization
In this Essay, I make a preliminary foray into this new narrative terrain, identifying several emerging legal storylines that have arisen in the wake of climate change disruptions and that I predict will prove influential in the coming years. In Part I, I discuss the ways in which new perceptions…
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The TFAMoplex—Conversion of the Mitochondrial Transcription Factor A into a DNA Transfection Agent
Non‐viral gene delivery agents, such as cationic lipids, polymers, and peptides, mainly rely on charge‐based and hydrophobic interactions for the condensation of DNA molecules into nanoparticles. The human protein mitochondrial transcription factor A (TFAM), on the other hand, has evolved to form…
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The intracellular visualization of exogenous DNA in fluorescence microscopy
In the development of non-viral gene delivery vectors, it is essential to reliably localize and quantify transfected DNA inside the cell. To track DNA, fluorescence microscopy methods are commonly applied. These mostly rely on fluorescently labeled DNA, DNA binding proteins fused to a fluorescent…
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DNA unchained: two assays to discover and study inhibitors of the DNA clustering function of barrier-to-autointegration factor
The protein barrier-to-autointegration factor (BAF) and its interaction partners, the LEM (LAP2B, emerin, MAN1)-domain proteins, constitute a powerful cytoplasmic DNA defense mechanism. Invading DNA molecules are quickly bound by the BAF system and trapped in membrane compartments. This decreases…
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Ligand-based CAR design enhances durability of CD70-targeted CAR T cells in glioblastoma
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Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication
This paper reports an effective protocol to encapsulate native protein/DNA complexes into unilamellar vesicles composed of natural lipids without the use of organic solvents, in physiological buffers, and at low protein/DNA concentrations. DNA compaction is achieved with the human mitochondrial…
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A dedicated cytoplasmic container collects extrachromosomal DNA away from the mammalian nucleus
Expression from transfected plasmid DNA is generally transient, but it is unclear what process terminates it. We show that DNA entering mammalian cells is rapidly surrounded by a double membrane in the cytoplasm, in some cases after leaving the nucleus. This cytoplasmic container, termed exclusome…
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VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection
In non-viral gene delivery, DNA entering the cytoplasm is rapidly bound by barrier-to-autointegration factor (BAF) and surrounded by nuclear envelope-like membranes. This process is thought to mitigate the translocation of the DNA from the cytoplasm through the nuclear pores limiting the…
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A microparticulate based formulation to protect therapeutic enzymes from proteolytic digestion: phenylalanine ammonia lyase as case study
Phenylketonuria is a genetic disorder affecting the metabolism of phenylalanine (phe) due to a deficiency in the enzyme phenylalanine hydroxylase. This disorder is characterized by an elevated phe blood level, which can lead to severe intellectual disabilities in newborns. The current strategy to…
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Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery
Gene delivery systems must overcome multiple barriers, with endosomal escape representing a prominent obstacle. We have previously shown that a bacterial phospholipase C (PLC) enabled endosomal escape of a non‐viral protein‐based DNA delivery system termed TFAMoplex. Building upon this, this work…
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Susceptibility- and T2*-weighted MRI features of CNS large B-cell lymphoma in a large single-center cohort: prevalence, patterns, and clinical associations
PURPOSE: The prevalence of susceptibility effects (SE) on T2*-weighted imaging (WI) and susceptibility-WI (SWI) in primary large B-cell lymphoma (IP-LBCL) of the central nervous system (CNS) and diffuse LBCL (DLBCL) with secondary CNS lymphoma (SCNSL) remains debated. This study aimed to clarify SE…
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Addition of Anti-Angiogenetic Therapy with Bevacizumab to Chemo- and Radiotherapy for Leptomeningeal Metastases in Primary Brain Tumors
Leptomeningeal dissemination of a primary brain tumor is a condition which is challenging to treat, as it often occurs in rather late disease stages in highly pretreated patients. Its prognosis is dismal and there is still no accepted standard of care. We report here a good clinical effect with a…
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Bevacizumab for Patients with Recurrent Gliomas Presenting with a Gliomatosis Cerebri Growth Pattern
Bevacizumab has been shown to improve progression-free survival and neurologic function, but failed to improve overall survival in newly diagnosed glioblastoma and at first recurrence. Nonetheless, bevacizumab is widely used in patients with recurrent glioma. However, its use in patients with…
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Evaluating the Effects of Fossil Fuel Supply Projects on Greenhouse Gas Emissions and Climate Change Under NEPA
Despite the high certainty of our looming climate catastrophe, fossil fuel production and consumption, and the greenhouse gas emissions that result, are increasing. In the United States, fossil fuel production reached record levels in 2018, and oil and gas pipelines are being constructed at an…
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Quantification of skin wound tension using a newly designed wound tensiometer
Objective To (i) quantitatively measure wound tension in experimental skin wounds using a newly developed wound tensiometer and (ii) establish reference values for primary skin wound closure in medium- and large-breed dogs. Study design Experimental cadaveric study. Animal population Nineteen dogs…
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Closing the gap: Nonviral TFAMoplex transfection boosted by bZIP domains compared to AAV-mediated transduction
The TFAMoplex is a nanoparticulate gene delivery system based on the mitochondrial transcription factor A (TFAM) protein, which can be engineered with various functional domains to enhance plasmid DNA transfection. In this study, we aimed at improving the TFAMoplex system by incorporating basic…