24 hasil untuk "June Simpson"
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Artikel Jurnal
Amplified fragment length polymorphisms (AFLP's)
AFLP es una técnica que combina PCR y análisis de fragmentos de restricción para detectar polimorfismos debidos a cambios en, o cerca de sitios de enzimas de restricción. La técnica detecta múltiples loci polimórficos a través del genoma. Son útiles para generar huellas genéticas o para mapeo. Las…
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Marcadores moleculares
Los marcadores moleculares son una herramienta necesaria en muchos campos de la biología, incluyendo; bio-medicina, mejoramiento, ciencias forenses y estudios de diversidad. Además se utilizan para localizar y aislar genes de interés. Ya existe una gran variedad de técnicas para llevar a cabo…
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The Sweet Taste of Adapting to the Desert: Fructan Metabolism in Agave Species
Over 70% of Agave species, (159 of 206) are found in Mexico and are well adapted to survive under hot, arid conditions, often in marginal terrain, due to a unique combination of morphological and physiological attributes. In the pre-Columbian era agaves were also key to human adaptation to desert…
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Advances in the Micropropagation and Genetic Transformation of Agave Species
The Agave genus is composed of approximately 210 species distributed from south United States to Colombia and Venezuela. Numerous Agave species have been used for the preparation of alcoholic beverages and have attracted interest in the pharmaceutical and food industry. Despite their economic…
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Transcriptome analysis of bolting in A. tequilana reveals roles for florigen, MADS, fructans and gibberellins
Abstract Background Reliable indicators for the onset of flowering are not available for most perennial monocarpic species, representing a drawback for crops such as bamboo, agave and banana. The ability to predict and control the transition to the reproductive stage in A. tequilana would represent…
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Genetic variation in the emblematic Puya raimondii (Bromeliaceae) from Huascarán National Park, Peru
Puya raimondii, the giant Peruvian and Bolivian terrestrial bromeliad, is an emblematic endemic Andean species well represented in Huascarán National Park in Peru. This park is the largest reserve of puna (high altitude plateau) vegetation. The objective of this study is to report on genetic…
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Molecular and Functional Characterization of Novel Fructosyltransferases and Invertases from Agave tequilana
Fructans are the main storage polysaccharides found in Agave species. The synthesis of these complex carbohydrates relies on the activities of specific fructosyltransferase enzymes closely related to the hydrolytic invertases. Analysis of Agave tequilana transcriptome data led to the identification…
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Analysis of Gene Expression and Physiological Responses in Three Mexican Maize Landraces under Drought Stress and Recovery Irrigation
Background: Drought is one of the major constraints for plant productivity worldwide. Different mechanisms of drought-tolerance have been reported for several plant species including maize. However, the differences in global gene expression between drought-tolerant and susceptible genotypes and…
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Comparative transcriptome analysis suggests convergent evolution of desiccation tolerance in Selaginella species
Abstract Background Desiccation tolerant Selaginella species evolved to survive extreme environmental conditions. Studies to determine the mechanisms involved in the acquisition of desiccation tolerance (DT) have focused on only a few Selaginella species. Due to the large diversity in morphology…
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Analysis and Optimization of Bulk DNA Sampling with Binary Scoring for Germplasm Characterization
The strategy of bulk DNA sampling has been a valuable method for studying large numbers of individuals through genetic markers. The application of this strategy for discrimination among germplasm sources was analyzed through information theory, considering the case of polymorphic alleles scored…
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Localization and Composition of Fructans in Stem and Rhizome of Agave tequilana Weber var. azul
Methodology combining mass spectrometry imaging (MSI) with ion mobility separation (IMS) has emerged as a biological imaging technique due to its versatility, sensitivity and label-free approach. This technique has been shown to separate isomeric compounds such as lipids, amino acids, carboxylic…
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Evidence for Light and Tissue Specific Regulation of Genes Involved in Fructan Metabolism in Agave tequilana
Plant Glycoside Hydrolase Family 32 (PGHF32) contains the fructosyltransferases and fructan exohydrolase enzymes responsible for fructan metabolism, in addition to closely related vacuolar and cell wall acid invertases. Agave species produce complex and dynamic fructan molecules (agavins) requiring…
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Criteria for prioritizing selection of Mexican maize landrace accessions for conservation in situ or ex situ based on phylogenetic analysis
IntroductionA combination of factors including migration, climate change and the encroachment of growing cities are associated with the extinction of species and a reduction in biodiversity. In Mexico maize landraces are an important source of germplasm that could be invaluable for future breeding…
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Comparative Genomic Analyses of Colletotrichum lindemuthianum Pathotypes with Different Virulence Levels and Lifestyles
Colletotrichum lindemuthianum is the most frequent pathogenic fungus of the common bean Phaseolus vulgaris. This filamentous fungus employs a hemibiotrophic nutrition/infection strategy, which is characteristic of many Colletotrichum species. Due to host–pathogen coevolution, C. lindemuthianum…
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High Genetic and Epigenetic Stability in Coffea arabica Plants Derived from Embryogenic Suspensions and Secondary Embryogenesis as Revealed by AFLP, MSAP and the Phenotypic Variation Rate
Embryogenic suspensions that involve extensive cell division are risky in respect to genome and epigenome instability. Elevated frequencies of somaclonal variation in embryogenic suspension-derived plants were reported in many species, including coffee. This problem could be overcome by using…
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Genetic diversity of Cuban pineapple germplasm assessed by AFLP Markers
The Cuban pineapple germplasm collection represents the genetic diversity of pineapple cultivated in that country and includes other important genotypes obtained from the germplasm collections in Brazil and Martinique. The collection has previously been characterized with morphological descriptors…