33 results for "Muhammad ASHRAF"
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Reversal of Ischemic Cardiomyopathy with Sca-1 + Stem Cells Modified with Multiple Growth Factors
Background: We hypothesized that bone marrow derived Sca-1+ stem cells (BM Sca-1+) transduced with multiple therapeutic cytokines with diverse effects will induce faster angiomyogenic differentiation in the infarcted myocardium. Methods and Results: BM Sca-1+ were purified from transgenic male mice…
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Identification and Characterization of a Novel Multipotent Sub-Population of Sca-1 + Cardiac Progenitor Cells for Myocardial Regeneration
Methods and Results: The cardiac stem/progenitor cells from adult mice were seeded at low density in serum-free medium. The colonies thus obtained were expanded separately and assessed for expression of stem cell antigen-1 (Sca-1). Two colonies each with high Sca-1 (CSH1; 95.9%; CSH2; 90.6%) and…
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Efficient Non-Viral Reprogramming of Myoblasts to Stemness with a Single Small Molecule to Generate Cardiac Progenitor Cells
Methods and Results: SMs from young male Oct3/4-GFP+ transgenic mouse were treated with DNA methyltransferase (DNMT) inhibitor, RG108. Two weeks later, GFP+ colonies of SM derived iPS cells (SiPS) expressing GFP and with morphological similarity of mouse embryonic stem (ESCs) were formed and…
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microRNA-150 Regulates Mobilization and Migration of Bone Marrow-Derived Mononuclear Cells by Targeting Cxcr4
The interaction between chemokine receptor type 4 (CXCR4) and its ligand, stromal cell-derived factor (SDF)-1, plays an important role in stem cell mobilization and migration in ischemic tissues. MicroRNAs (miRs) are key regulators of stem cell function and are involved in regulation of stem cell…
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Non-Invasive Evaluation of Heart Function with Four-Dimensional Echocardiography
Background: The aim of this study is to assess the accuracy and feasibility of left ventricular systolic function determined by four-dimensional echocardiography (4DE). Methods: Latex balloons were sewn into the left ventricle (LV) of 20 freshly harvested pig hearts which were then passively driven…
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Ischemic Preconditioning Potentiates the Protective Effect of Stem Cells through Secretion of Exosomes by Targeting Mecp2 via miR-22
Mesenchymal stem cells (MSCs) have potential application for the treatment of ischemic heart diseases. Besides differentiation properties, MSCs protect ischemic cardiomyocytes by secretion of paracrine factors. In this study, we found exosomes enriched with miR-22 were secreted by MSCs following…
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Mitigation of Variability among 3D Echocardiography-Derived Regional Strain Values Acquired by Multiple Ultrasound Systems by Vendor Independent Analysis
Introduction: This study compared the variability of 3D echo derived circumferential and longitudinal strain values computed from vendor-specific and vendor-independent analyses of images acquired using ultrasound systems from different vendors. Methods: Ten freshly harvested porcine hearts were…
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Effects of temperature dependent viscosity and thermal conductivity on natural convection flow along a curved surface in the presence of exothermic catalytic chemical reaction
The natural convection boundary layer flow of a viscous incompressible fluid with temperature dependent viscosity and thermal conductivity in the presence of exothermic catalytic chemical reaction along a curved surface has been investigated. The governing non dimensional form of equations is…
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Coverage and Quality of Antenatal Care Provided at Primary Health Care Facilities in the ‘Punjab’ Province of ‘Pakistan’
Background: Antenatal care is a very important component of maternal health services. It provides the opportunity to learn about risks associated with pregnancy and guides to plan the place of deliveries thereby preventing maternal and infant morbidity and mortality. In ‘Pakistan’ antenatal…
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Heat source and sink effects on periodic mixed convection flow along the electrically conducting cone inserted in porous medium
The system of partial differential equations governing the unsteady hydromagnetic boundary-layer flow along an electrically conducting cone embedded in porous medium in the presence of thermal buoyancy, magnetic field, heat source and sink effects are formulated. These equations are solved…
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Sonic Hedgehog Gene Delivery to the Rodent Heart Promotes Angiogenesis via iNOS/Netrin-1/PKC Pathway
Background: We hypothesized that genetic modification of mesenchymal stem cells (MSCs) with Sonic Hedgehog (Shh) transgene, a morphogen during embryonic development and embryonic and adult stem cell growth, improved their survival and angiogenic potential in the ischemic heart via iNOS/netrin/PKC…
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Sugar beet extract rich in glycine betaine modulates oxidative defense system and key physiological characteristics of maize under water-deficit stress
Now-a-days, plant-based extracts, as a cheap source of growth activators, are being widely used to treat plants grown under extreme climatic conditions. So, a trial was conducted to assess the response of two maize (Zea mays L.) varieties, Sadaf (drought tolerant) and Sultan (drought sensitive) to…
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Development of sustainable alkali activated composite incorporated with sugarcane bagasse ash and polyvinyl alcohol fibers
The infrastructure boom has driven up cement demand to 30 billion tons annually. To address this and promote sustainable construction, researchers are developing solutions for carbon-neutral building practices, aiming to transform industrial waste into an eco-friendly alternative. This study aims…
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Development of innovative alkali activated paste reinforced with polyethylene fibers for concrete crack repair
Concrete structures are susceptible to cracking, which can compromise their integrity and durability. Repairing them with ordinary Portland cement (OPC) paste causes shrinkage cracks to appear in the repaired surface. Alkali-activated binders offer a promising solution for repairing such cracks…
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Thiamin stimulates growth, yield quality and key biochemical processes of cauliflower ( Brassica oleracea L. var. Botrytis) under arid conditions
Thiamin is a crucial vitamin with a vast variety of anti-oxidative and physiological roles in plants subjected to abiotic stresses. We examined the efficiency of foliar-applied thiamin (50 and 100 mM) on growth, yield quality and key-biochemical characteristics of two cultivars (FD1 and FD3) of…
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Methionine-induced regulation of growth, secondary metabolites and oxidative defense system in sunflower ( Helianthus annuus L.) plants subjected to water deficit stress
Optimum water availability at different growth stages is one the major prerequisites of best growth and yield production of plants. Exogenous application of plant growth regulators considered effective for normal functioning of plants under water-deficit conditions. A study was conducted to examine…
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Physicians’ awareness of oral-systemic links and its association with physician-reported patient referral to dentists
Introduction: Physicians promote oral health by screening for oral pathologies, providing emergency help, and referring patients to dentists. The literature consistently reports a robust association between periodontal disease and systemic diseases. However, it is not fully known if physicians in…
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Improving the clinical recognition, prognosis, and treatment of melioidosis through epidemiology and clinical findings: The Sabah perspective
Introduction: Melioidosis is a deadly endemic disease in northern Australia and Southeast Asia, including Sabah, Malaysia, which is caused by the bacterium Burkholderia pseudomallei. It contributes to high fatality rates, mainly due to misdiagnosis leading to the wrong treatment being administered…
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Estimation of the domestic water demand‒supply scenario and its key driving factors in the Islamabad-Rawalpindi Metropolitan Area, Pakistan
Domestic water availability is intricately linked with a growing population, urbanization, and associated land use/land cover change (LULCC). Precise and periodic assessments of water supply and demand are imperative for the sustainability of urban ecosystems. The present study provides a…