11 results for "Nicholas Rhind"

The three most important things about origins: location, location, location

Nicholas Rhind

Molecular Systems Biology, Vol. 10 No. 4 · 2014 · English · DOAJ Scopus

Abstract The reasons why some DNA replication origins fire earlier than others have remained elusive. New work by Gindin et al suggests that the distribution of replication origins, not their timing per se, is the major determinant of the timing of genome replication in human cells.

pomBseen: An automated pipeline for analysis of fission yeast images.

Makoto Ohira; Nicholas Rhind

PLoS ONE, Vol. 18 No. 9 · 2023 · English · DOAJ Scopus

Fission yeast is a model organism widely used for studies of eukaryotic cell biology. As such, it is subject to bright-field and fluorescent microscopy. Manual analysis of such data can be laborious and subjective. Therefore, we have developed pomBseen, an image analysis pipeline for the…

Also available at: PLOS

The capacity of origins to load MCM establishes replication timing patterns.

Livio Dukaj; Nicholas Rhind

PLoS Genetics, Vol. 17 No. 3 · 2021 · English · DOAJ Scopus

Loading of the MCM replicative helicase at origins of replication is a highly regulated process that precedes DNA replication in all eukaryotes. The stoichiometry of MCM loaded at origins has been proposed to be a key determinant of when those origins initiate replication during S phase…

Also available at: PLOS

The role of specific checkpoint-induced S-phase transcripts in resistance to replicative stress.

Chaitali Dutta; Nicholas Rhind

PLoS ONE, Vol. 4 No. 9 · 2009 · English · DOAJ Scopus

Checkpoint activation during S phase modulates transcription. In response to replication arrest, the fission yeast Cds1 checkpoint kinase maintains the normal S-phase transcriptional program by regulating MBF, the S-phase transcription factor. We show that similar regulation occurs in response to…

Also available at: PLOS

Replication fork slowing and stalling are distinct, checkpoint-independent consequences of replicating damaged DNA.

Divya Ramalingam Iyer; Nicholas Rhind

PLoS Genetics, Vol. 13 No. 8 · 2017 · English · DOAJ Scopus

In response to DNA damage during S phase, cells slow DNA replication. This slowing is orchestrated by the intra-S checkpoint and involves inhibition of origin firing and reduction of replication fork speed. Slowing of replication allows for tolerance of DNA damage and suppresses genomic…

Also available at: PLOS

Modeling genome‐wide replication kinetics reveals a mechanism for regulation of replication timing

Scott Cheng‐Hsin Yang; Nicholas Rhind; John Bechhoefer

Molecular Systems Biology, Vol. 6 No. 1 · 2010 · English · DOAJ Scopus

Abstract Microarrays are powerful tools to probe genome‐wide replication kinetics. The rich data sets that result contain more information than has been extracted by current methods of analysis. In this paper, we present an analytical model that incorporates probabilistic initiation of origins and…

A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus

Guo-Song Jia; Wen-Cai Zhang; Yue Liang et al.

G3: Genes, Genomes, Genetics, Vol. 13 No. 4 · 2023 · English · DOAJ Scopus

AbstractFission yeasts are an ancient group of fungal species that diverged from each other from tens to hundreds of million years ago. Among them is the preeminent model organism Schizosaccharomyces pombeS. pombeS. japonicusS. octosporusS. cryophilusS. osmophilusS. osmophilusS. octosporusS…