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Indonesian Journal of Biotechnology
Scopus
ISSN 0853-8654 · Universitas Gadjah Mada, Yogyakarta · 537 artikel di indeks
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Artikel Jurnal
Integrated metabolite profiling and in silico screening reveal the cholesterol‐modulating potential of tauco, an Indonesian fermented soybean paste
Tauco, a traditional Indonesian fermented soybean paste, is a promising yet under‐characterized source of dietary bioactives. To systematically evaluate its functional potential, we first identified ultrasonic‐assisted extraction (UAE) as the most efficient method, yielding the highest total…
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Artikel Jurnal
Recombinant expression and preliminary characterization of a synthetic L‐asparaginase from the marine bacterium Pseudoalteromonas tetraodonis GFC in Escherichia coli BL21 (DE3)
The marine environment represents a promising source of diverse enzymes produced by marine microorganisms, including L‐asparaginase. This has been widely studied due to its ability to hydrolyze extracellular L‐asparagine, an amino acid required for the growth of certain cancer cells. In this study…
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Detection of homologous plastic PET‐degrading enzyme‐encoding DNA from enriched plastic‐contaminated soil samples
Piscinibacter sakaiensis, first isolated in Japan, is the only well‐characterized bacterium known to possess both PETase and MHETase, enabling complete polyethylene terephthalate (PET) degradation. To date, no additional habitats for the species have been reported. This study aims to identify…
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The role of astaxanthin‐Cu2+ in stabilizing glycated human serum albumin for type 2 diabetes mellitus management: a computational approach
Type 2 diabetes mellitus (T2DM) leads to the non‐enzymatic glycation of proteins, resulting in the formation of advanced glycation end products (AGEs), which contribute to diabetic complications. Human serum albumin (HSA), a major plasma protein, undergoes structural alterations upon glycation…
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Artikel Jurnal
Decolorization of naphthol batik effluents using immobilized enzyme of Aspergillus sp. GPN and zeolite‐activated carbon in a wastewater treatment plant (WWTP)
Industrial batik wastewater containing naphthol dyes is persistent, dark in color, and contains complex organic compounds that have the potential to pollute aquatic environments. Conventional physicochemical treatment methods are often ineffective in degrading resistant aromatic compounds and…
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Artikel Jurnal
Decoding the role of tannic acid in wound healing: a dual‐action mechanism linking IL‐1β modulation and FGF‐driven tissue repair
Tannic acid (TA) has been shown in a previous study to expedite cutaneous wound healing in rats; however, the precise mechanism by which it operates remains poorly understood. This research evaluates the effects of TA on wound healing using both in vitro and in silico methods. In vitro, its…
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Chitosan nanoparticle‐mediated delivery of anti‐miR‐203a‐3p for 4T1 triple‐negative breast cancer
Anti‐miR molecules can suppress specific microRNA (miRNA) functions within critical signaling pathways. Chitosan acts as a delivery system for miRNAs; therefore, encapsulating miR‐203a‐3p is essential for targeted delivery and biological activity. This study investigates the impact of…
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Network pharmacology‐based exploration of gut microbiota‐derived metabolites for type‐2 diabetes
Probiotics confer health benefits and have been investigated for their potential therapeutic properties in type‐2 diabetes (T2D) treatment. This study employs a network pharmacology approach to explore gut microbiota‐derived metabolites that potentially alleviate T2D. Several strains and species of…
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Artikel Jurnal
Antibacterial activity of mycelial extract from a local fungus, Sclerotium rolfsii
Mycelium‐to‐sclerotium differentiation in fungi involves not only morphological but also biochemical changes throughout the process, which may contribute to their persistence and be a possible source of bioactive compounds. This study aims to evaluate the antibacterial activity and identify the…
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Artikel Jurnal
In vitro evaluation, molecular docking, and molecular dynamics studies of resorcinol derivatives against yeast α‐glucosidase
Nine resorcinol derivatives were evaluated for their ability to inhibit yeast α‐glucosidase using the in vitro method. Three molecular docking programs (Autodock Vina, Autodock4 and DockThor) were employed to determine the binding energies. The results showed that two resorcinol derivatives…
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Biodegradable plant pots made from dried banana pseudo‐stems enriched with a Bacillus sp.‐biochar composite as an eco‐friendly alternative to plastic pots
Agricultural plastic waste is a major environmental pollutant due to its non‐biodegradable nature. This study discusses the production of biodegradable pots (bio‐pots) using a biochar composed of banana pseudo‐stems and Bacillus sp. The isolated Bacillus sp. produced indole‐3‐acetic acid (IAA)…
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Extracellular alpha‐amylase from halophilic bacteria Marinobacter sp. LES TG5: Isolation, optimization, and characterization
The growing demand for stable and effective enzymes requires the discovery of novel microbial producers. Alpha‐amylase is an enzyme in high demand by various industries; however, the discovery of novel and stable alpha‐amylase producers remains limited. This study aims to isolate, optimize, and…
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Artikel Jurnal
Orchid‐associated endophytic Bacillus mediates Fusarium suppression and promotes in vitro regeneration of banana plantlets via culture supernatant
The application of Fusarium‐antagonistic endophytic bacteria with plant growth‐promoting traits offers an effective method to enhance the success of banana plantlet tissue culture while combating Fusarium wilt disease caused by Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4) (VCG 01213)…
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Anti‐inflammatory properties of conditioned medium from human Wharton’s jelly mesenchymal stem cells
Acute respiratory distress syndrome (ARDS) is a critical respiratory dysfunction triggered by intense in‐ flammation, microvascular damage, and increased epithelial and pulmonary vascular permeability. Human Wharton’s jelly mesenchymal stem cells (hWJMSCs) possess regenerative and anti‐inflammatory…
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Artikel Jurnal
Enzyme and hormone activities related to phosphorus uptake limitation in oil palm (Elaeis guineensis Jacq.)
Phosphorus (P) is an essential element for oil palm growth and development. Acid phosphatase (Apase) and Pti‐interacting serine/threonine kinase are two enzymes which enzymes confirmed to be related to P‐uptake in oil palm, therefore their activities in oil palm treated with P‐limitation need to be…
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Artikel Jurnal
Metagenomic detection and biochemical characterization of a Streptomyces tyrosinase from Mesopotamian marsh soils: Implications for phenolic turnover
Wetland soils in southern Iraq store large carbon pools and contain abundant phenolics that can modulate microbial decomposition. In this study, we investigate bacterial tyrosinases (TYRs), type III copper enzymes that oxidize mono‐ and diphenols, in Mesopotamian marsh soils using a combined…
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Artikel Jurnal
Evaluation of synthetic‐gene recombinant LipL32 antigen for IgM ELISA detection of Leptospira infection
Leptospirosis presents with nonspecific clinical features and requires time‐consuming laboratory tests for gold standard diagnosis. This study aims to design and characterize the recombinant LipL32 from synthetic gene and assess its performance as an antigen for detecting leptospirosis. The antigen…
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Artikel Jurnal
Optimization of methanol‐induced expression and His‐tag purification of Saccharomycopsis fibuligera R64 mutant α‐amylase in Pichia pastoris
The Sfamy R64 α‐amylase mutant from Saccharomycopsis fibuligera was expressed in Pichia pastoris to explore its industrial potential. The gene encoding the mutant enzyme was cloned into the pPICZαA vector and transformed into P. pastoris SMD1168. Optimal expression was achieved at 1.5% methanol…
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Artikel Jurnal
Integrated omics for increasing plant production and health‐related nutrition under extreme conditions: The Indonesia perspective
Despite Indonesia being a megadiverse country that provides germplasm for breeding to produce improved future varieties, significant threats are faced related to biodiversity extinction. Such threats, for example habitat degradation and climate change, which lead to extreme conditions, must be…
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Artikel Jurnal
Identification and genetic study of lactic acid bacteria from intestine of domestic chickens (Gallus gallus domesticus)
The gastrointestinal tract is one of the habitats of lactic acid bacteria (LAB). This research aims to identify the LAB isolated from the intestines of domestic chickens (Gallus gallus domesticus) based on phenotypic and genotypic characteristics. The LAB was cultivated on enrichment media MRSA +…