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The Journal of Reproduction and Development
Scopus
ISSN 0916-8818 · The Society for Reproduction and Development · 486 artikel di indeks
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Artikel Jurnal
Optimization of a comprehensive metabolomic analysis system for characterizing metabolic alterations in the cauda epididymis of mature and juvenile mice
The cauda epididymis protects and stores mature sperm in mammals. Recently, comprehensive transcriptomic and proteomic analyses have been conducted to understand its molecular functions; however, fundamental information on its metabolites has not been reported. In this study, we optimized a system…
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Effect of post-insemination GnRH analogue administration on pregnancy rates in repeat-breeder cows: Comparison of dosage and frequency
Repeat-breeder cows (defined as having > 3 inseminations) significantly impact dairy profitability worldwide. This study evaluated the clinical efficacy of two elevated doses of the GnRH analogue, dephereline, administered during the early luteal phase (5–7 days post-insemination) to improve…
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Holding temperature to transport immature bovine oocytes in collection media critically affects their developmental competence
We investigated the holding condition in collection media to establish transportation system of immature bovine cumulus-oocyte complexes (COCs). We examined holding temperature (38°C or 20°C), basal media (Lactated Ringer's solution and TCM199) and supplementation of hypoxanthine, an inhibitor of…
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Effect of paternal resveratrol intake on mitochondria and telomere length in mouse embryos and offspring
Advanced paternal age affects embryonic development and offspring phenotypes. We previously demonstrated that resveratrol prevents age-associated declines in the mitochondrial DNA copy number (mt-cn) and telomere length (TL) in embryos. The present study was performed to investigate the effects of…
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Ovarian and follicular culture enables the production of fertile oocytes in C57BL/6 mice
In vitro experiments have become a powerful alternative to in vivo approaches for analyzing gene functions and molecular mechanisms. However, successful in vitro oogenesis using the C57BL/6 strain–widely used as a standard mouse model in mammalian studies–has not yet been reported. In this study…
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Preimplantation culture conditions and later-life outcomes: implications for evaluating embryo culture media
Environmental conditions during early development are critical determinants of lifelong health, as described by the Developmental Origins of Health and Disease (DOHaD) concept. Preimplantation embryos develop entirely within artificial culture systems during assisted reproduction, yet current media…
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Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR
Reliable pre-implantation sex determination and genetic screening enables informed embryo transfer decisions in equine breeding while avoiding later interventions. We developed a streamlined workflow that couples rapid whole genome amplification (WGA) with a multiplex real-time PCR targeting ETSTY5…
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Genomic evaluation and gene expression analysis of preimplantation embryos for enhancing cattle breeding and in vitro embryo production
Genomic selection using genomic estimated breeding values (GEBVs) based on single nucleotide polymorphism (SNP) genotyping has been widely adopted for efficient breeding of cattle. Recent advances in DNA analysis technologies have enabled comprehensive SNP genotyping using a small number of cells…
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GM-CSF increases glucose uptake and mitochondrial activity and reduces reactive oxygen species levels in mouse cumulus-oocyte complexes during oocyte in vitro maturation
We have previously reported that the addition of granulocyte macrophage-colony stimulating factor (GM-CSF) during oocyte in vitro maturation (IVM) can increase embryo development in mice. The present study examined glucose uptake, mitochondrial activity, reactive oxygen species (ROS) levels, and…
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N-myristoyltransferase 1, a key gene for protein N-myristoylation, is dispensable for fertility in male mice
N-Myristoyltransferase 1 (NMT1), the predominant enzyme catalyzing myristoylation of proteins, is involved in various biological processes, including early embryonic development, immune responses, apoptosis, cellular homeostasis, tumorigenesis, and infection, with therapeutic potential in viral and…
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Artikel Jurnal
Monosodium glutamate protects DNA of freeze-dried somatic cells and improves development of somatic cell nuclear transfer embryos
Freeze-dried (FD) somatic cells serve as a novel method for preserving animal genetic resources; however, the efficiency of cloned mice production from FD somatic cells remains low, owing to severe nuclear damage induced by the freeze-drying. In this study, we aimed to mitigate…
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Identification of the optimal manipulation medium temperature for in vitro handling of oocytes and embryos during in vitro maturation, parthenogenesis, and in vitro culture in pigs
Exposure of cumulus–oocyte complexes (COCs), mature oocytes, and preimplantation embryos to subphysiological temperatures during in vitro maturation (IVM), parthenogenesis (PG), and in vitro culture (IVC) has been shown to impair oocyte maturation, embryogenesis, and developmental competence…
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Interactions among progesterone, estradiol, and γ-aminobutyric acid in rat spermatozoal hyperactivation
Our previous study in hamsters indicated that progesterone (P) induces enhancement of spermatozoal hyperactivation, while 17β-estradiol (Eβ) and γ-aminobutyric acid (GABA) suppress this enhancement. Since P has also been shown to enhance hyperactivation of rat spermatozoa in another study conducted…
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Studies on gestational factors affecting postpartum reproductive performance in dairy cows and the health and reproductive potential of their offspring
Improving and sustaining the health and productivity of dairy cows require integrated strategies that combine management and appropriate nutrition. This review adopts two complementary perspectives: optimizing periparturient management to support high productivity without compromising animal health…
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Effects of thiol compounds on DNA-damage detection and in vitro fertilization outcomes in bovine sperm
Reduced glutathione (GSH), a thiol compound, plays an important role in protecting sperm from excessive levels of reactive oxygen species. Our previous study demonstrated that GSH supplementation was associated with improved in vitro fertilization (IVF) outcomes. However, GSH supplementation also…
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Germ cells are hidden contributors to sex differences in vertebrate lifespan
Aging is a complex biological process whose regulatory mechanisms remain incompletely understood. Accumulating evidence indicates that germ cells play pivotal roles in the systemic regulation of aging. The link between germ cells and somatic aging was first established in invertebrate models, where…
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Malonic acid promotes glycolytic activity in cumulus cells and improves the quality of bovine oocytes matured in vitro
Malonic acid (MA) is a dicarboxylic acid that inhibits the mitochondrial electron transport chain. Cumulus cells surrounding human oocytes that achieve conception after in vitro fertilization (IVF) contain higher levels of MA than those that do not. Although MA has no effect on embryo developmental…
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Beyond a single signal: How can bovine pregnancy be established without IFNT?
Interferon tau (IFNT) has been regarded as the dominant conceptus signal for maternal recognition of pregnancy (MRP) in ruminants. In cattle, IFNT is classically proposed to prevent luteolysis by suppressing uterine luteolytic pulses of prostaglandin F2α (PGF2α). IFNT also induces endometrial…
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Deletion of Ybx1 results in germ cell hypoplasia in mouse embryos
Several evolutionarily conserved genes have been reported to be involved in gametogenesis. Y-box binding proteins, which have a conserved nucleotide-binding cold-shock domain, have been reported in both vertebrates and invertebrates. Although homologues of Ybx1 in Drosophila and zebrafish play…
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Artikel Jurnal
Detection of sex chromosome-specific DNA sequences by polymerase chain reaction using hair DNA in cattle
To establish a simpler polymerase chain reaction (PCR) strategy for detecting sex chromosome-specific DNA sequences in cattle, we verified the feasibility of using hair-derived DNA as a PCR template. In the control steer, multiplex PCR using hair-derived DNA resulted in the amplification of two…