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Journal of Clinical Microbiology
Scopus
ISSN 1098-660X · American Society for Microbiology · 509 artikel di indeks
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Artikel Jurnal
Comparative study of plasma microbial cell-free DNA sequencing to culture and polymerase chain reaction in pediatric community-acquired pneumonia with parapneumonic effusion or empyema
ABSTRACT Plasma microbial cell-free DNA (mcfDNA) sequencing is a novel diagnostic tool for pediatric complicated community-acquired pneumonia (cCAP). However, rigorous evaluation of real-world mcfDNA performance is lacking. We compared mcfDNA sequencing to a composite reference standard consisting…
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Artikel Jurnal
Antifungal susceptibility and molecular characterization of clinical and environmental isolates of Schizophyllum commune
ABSTRACT Schizophyllum commune is a cosmopolitan, saprophytic basidiomycete known to cause respiratory tract infections following spore inhalation. Management of such infections remained poorly defined, and antifungal susceptibility testing may provide valuable guidance for therapeutic decisions…
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Artikel Jurnal
Analytical and clinical performance of in-house and commercial real-time PCR assays for diagnosing L. infantum visceral leishmaniasis: a study from a hub center in Northern Italy
ABSTRACT Molecular methods are increasingly used to diagnose visceral leishmaniasis (VL), a life-threatening disease caused by Leishmania infantum in the Mediterranean basin. In this study, we compared the analytical and clinical performances of three real-time polymerase chain reaction (PCR)…
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Artikel Jurnal
Clinical performance of two commercial PCR assays for the detection of macrolide resistance in Mycoplasma pneumoniae
ABSTRACT Macrolides are the first-line treatment for Mycoplasma pneumoniae infections; however, macrolide resistance has been reported. We evaluated the clinical performance of two commercial assays for detecting macrolide-resistant M. pneumoniae: the LightMix Modular Mycoplasma Macrolide (TIB…
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Artikel Jurnal
A tiled amplicon protocol for culture-free whole-genome sequencing of M. tuberculosis from clinical specimens
ABSTRACT Whole-genome sequencing of Mycobacterium tuberculosis can be a valuable tool for TB surveillance and treatment, providing insights into transmission patterns and comprehensive drug susceptibility testing. However, the slow growth of M. tuberculosis means traditional culture-based…
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Artikel Jurnal
Evaluation of KU-F40 automated microscope for parasitology: when artificial intelligence meets old school microscopy
ABSTRACT Intestinal parasitic infections (IPIs) have a worldwide distribution and have a major impact on health, work capacity, and economy in many countries. Light microscopy is still considered the reference method for IPI diagnosis but is labor-intensive. KU-F40, an automated feces analyzer…
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Artikel Jurnal
Revisiting genital leptospirosis in large animals: impacts on reproductive health, diagnostic challenges, and future directions
ABSTRACT Leptospirosis, caused by Leptospira spp. infection, is a globally significant zoonotic disease that affects a wide range of animals. Although renal colonization is well-documented, genital infection by leptospires remained less explored for decades, despite its impact on reproduction…
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Artikel Jurnal
HPV testing with 16/18 genotyping for risk stratification among women with normal cytology: a multicenter prospective cohort study from China
ABSTRACT To evaluate the clinical performance of Hybribio’s 14-type HPV real-time PCR with 16/18 genotyping (HBRT-H14) and its risk stratification utility among women with normal cytology (NILM). From 2017 to 2020, a multicenter cohort enrolled 8,401 women aged 30–64 years with NILM cytology…
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Artikel Jurnal
Spurious passage of Calodium hepaticum in human stools misidentified as Trichuris spp. infection in school-age children in Angola
ABSTRACT Calodium hepaticum (syn. Capillaria hepatica) is a zoonotic parasite of murid rodents and other mammals, while also being an important agent of hepatic capillariasis in humans. In a cross-sectional survey of schoolchildren across three provinces (Huambo, Uíge, and Zaire) in Angola, we…
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Artikel Jurnal
A standardized, genome-guided MLST scheme for Avibacterium paragallinarum: enhanced epidemiological typing and validation against existing methods
ABSTRACT Avibacterium paragallinarum, the causative agent of infectious coryza (IC), is an important respiratory pathogen of chickens with growing prevalence in commercial and backyard flocks. Current strain-typing methods, including classical serotyping and molecular approaches, such as ERIC-PCR…
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Artikel Jurnal
Characteristics of mycological criteria for the diagnosis of invasive mold infections in patients with severe burn injury
ABSTRACT Invasive fungal diseases (IFDs) are common and often fatal in severe burn patients due to skin barrier loss and immune dysfunction. However, current definitions of invasive mold infections are poorly adapted to this population. This study evaluated the characteristics of various diagnostic…
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Artikel Jurnal
Understanding recurrence in Mycobacterium avium complex pulmonary disease: genotypic strategies to support clinical decision-making
ABSTRACT Pulmonary disease caused by Mycobacterium avium complex (MAC-PD) is a chronic, recurrent disease, and its high recurrence rate after treatment makes clinical management difficult. Distinguishing whether recurrence is due to persistence of existing strains or reinfection with new strains is…
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Artikel Jurnal
Diagnosis of leptospirosis in animals: challenges and perspectives
ABSTRACT Leptospirosis is a life-threatening zoonotic disease with a major public and animal health impact. Annually, approximately 1.03 million people are affected by leptospirosis, but our understanding of its impact on animals is limited. The epidemiology, pathogenesis, clinical presentation…