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The EMBO Journal
Scopus
ISSN 1460-2075 · Springer Nature · 912 artikel di indeks
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Artikel Jurnal
Poxvirus dsDNA genomes differentially activate AIM2 or NLRP3 inflammasomes in human primary cells
Abstract The innate immune system is known for its ability to recognize cytosolic DNA as evidence of infection, but detailed studies of this process have been mostly limited to mice and cell lines. To investigate inflammasome responses in human primary cells, we used engineered viruses encoding the…
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Artikel Jurnal
RBM15B recognizes H3K79me2 to guide selective m6A-modification of mRNA and enhance oncoprotein translation in MLL-r leukemia
Abstract The distribution of N6-methyladenosine (m6A) controls its substrate RNA fate, playing key roles in various biological processes. However, the mechanism underlying site-selective m6A deposition of RNAs, especially in the start codon regions, and the role in epigenetic information…
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Artikel Jurnal
Molecular basis for the activation of Aurora A and Plk1 kinases during mitotic entry
Abstract The evolutionarily conserved, intrinsically disordered protein Bora is critical for initiating the activation of mitotic kinases. Once phosphorylated at Ser112 by Cyclin A-Cdk1 kinase, phospho-Bora activates unphosphorylated Aurora A kinase (AURKA), directing it towards Polo-like kinase 1…
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Artikel Jurnal
Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells
Abstract Quiescence is a cellular state defined by reversible cell-cycle arrest and diminished biosynthesis, particularly of nucleic acids and proteins. These features protect stem cells from proliferation-induced mutations, self-renewal exhaustion, and environmental insults. Despite relevance to…
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Artikel Jurnal
STIM1-containing contact sites promote direct calcium flux from the endoplasmic reticulum to mitochondria
Abstract STIM1 is a transmembrane protein localized in the endoplasmic reticulum (ER), where it acts as a calcium ion sensor, activating store-operated Ca2+ entry upon ER Ca2+ depletion. Via cellular calcium influx, STIM1 is thought to indirectly affect mitochondrial calcium content. Here we show…
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Artikel Jurnal
Molecular requirements for PLK1 activation by T-loop phosphorylation
Abstract Activation of PLK1, a master mitotic kinase, requires phosphorylation of its activation segment on Thr210, within a basic consensus sequence for Aurora kinases. Aurora B-dependent phosphorylation of Thr210 has been reported, but other evidence identified a strict requirement for the Aurora…
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Artikel Jurnal
TORC1-dependent translation drives chromatin remodeling during the germ-cell-to-maternal transition in Drosophila
Abstract Proper oogenesis requires a programmed transition from an undifferentiated germ-cell gene expression program to a maternal gene-expression state. While this process depends on the heterochromatin-mediated silencing of germ-cell genes, the upstream mechanisms that enforce this…
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Artikel Jurnal
Direct visualization and tracing of chromatin folding in the Drosophila embryo
Abstract Chromatin organization, through the assembly of DNA with histones and the folding of nucleosome chains, regulates DNA accessibility for transcription, DNA replication and repair. Although models derived from in vitro studies have proposed distinct nucleosome chain geometries, the…
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Artikel Jurnal
M18BP1 valency and a distributed interaction footprint determine epigenetic centromere specification in humans
Abstract The histone H3 variant CENP-A is considered an epigenetic landmark of centromeres. Its deposition reflects cell-cycle-regulated assembly of M18BP1, HJURP, and PLK1 on a divalent MIS18α/β scaffold. The localization determinants of this machinery remain poorly characterized. Here, we report…
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Artikel Jurnal
Direct receptor competition gates RGL2 proteolysis for seed germination timing in Arabidopsis
Abstract Seed germination is orchestrated by antagonistic gibberellin (GA) and abscisic acid (ABA) signals converging on the master germination repressor RGL2. Here, we unveil a receptor-competition paradigm where ABA receptors (PYLs) stabilize RGL2, both through direct physical interaction and…
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Artikel Jurnal
Unique territorial and compartmental organization of chromosomes in the holocentric silkworm
Abstract Hallmarks of multicellular eukaryotic genome organization are chromosome territories, compartments, and loop-extrusion-mediated structures, including TADs. However, these have mainly been observed in model organisms, and most eukaryotes remain unexplored. Using Hi-C in the silkworm Bombyx…
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Artikel Jurnal
Mitotic entry: Bora takes Polo to Aurora, and gives them a hug
Cell division involves the complete reorganization of the cellular architecture, requiring precise coordination of cellular processes in space and time, but how does this control system work at the atomic level? Three new studies offer a glimpse under the hood, revealing structural details of the…
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Artikel Jurnal
Single-nucleotide m⁶A mapping uncovers redundant YTHDF function in planarian progenitor fate selection
Abstract Cell fate decisions require tight regulation of gene expression. In planarians, highly regenerative flatworms, the mRNA modification N⁶-methyladenosine (m⁶A) modulates progenitor production and fate. However, the mechanisms governing m⁶A deposition in the planarian transcriptome, and the…
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Artikel Jurnal
Epromoters bind key stress-related transcription factors to regulate clusters of stress response genes
Abstract Cellular and environmental stress triggers the rapid and global reprogramming of gene transcription by coordinated recruitment of a limited number of key inducible transcription factors to cis-regulatory elements. Here, we performed a comprehensive analysis of different stress models and…
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Artikel Jurnal
Membrane curvature initiates Cdc42-FBP17-N-WASP clustering and actin nucleation
Abstract The architecture of actin networks at the cell surface is regulated by local membrane topology. However, how actin nucleation can respond sensitively to the degree of membrane curvature remains incompletely understood. Using nanolithography to precisely control local membrane curvature, we…
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Artikel Jurnal
The expanding roles of homologous recombination proteins in genome stability
Abstract Homologous recombination (HR) is traditionally portrayed as a DNA double-strand break repair pathway. However, emerging evidence positions RAD51, its partners BRCA1, BRCA2, and other HR factors at the core of a broader genome-maintenance network that operates by a “prevent and protect”…
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Artikel Jurnal
Bacterial ubiquitin ligase engineered for small molecule and protein target identification
Abstract The Legionella SidE effectors ubiquitinate host proteins independently of the canonical E1-E2 cascade. Here we engineer the SidE ligases to develop a modular proximity ligation approach for the identification of targets of small molecules and proteins, which we call SidBait. We validate…
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Artikel Jurnal
Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis
Abstract Despite being evolutionary distant, plants and animals exhibit a shared phenomenon during the transition from somatic-to-reproductive cell fate marked by extensive structural and compositional changes in chromatin. This chromatin reprogramming occurs in the plant SMCs (Spore Mother Cells)…
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Artikel Jurnal
The ATG8 E3-like ligases sense lysosomal damage and initiate ESCRT-mediated membrane repair
Abstract After damage from pathogenic, chemical or physical stress, endolysosomal membranes are repaired and resealed by the endosomal sorting complex required for transport (ESCRT) machinery, but how this membrane damage is sensed and translated into ESCRT recruitment is poorly understood. Here…
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Artikel Jurnal
Asymmetric envelope surface disposition of secreted protein YjbI controls bimodal antibiotic susceptibilities in C. crescentus
Abstract Cytoplasmic pentapeptide repeat proteins (PRPs) protect bacterial DNA gyrase from quinolone antibiotics. While some secreted PRPs are essential upon quinolone exposure, their role in the regulation of antibiotic resistance remains to be fully characterized. We show that a YjbI-type…