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Microbial Cell Factories
Scopus
ISSN 1475-2859 · BMC · 3.164 artikel di indeks
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Artikel Jurnal
Mechanism-guided control of intracellular alkalinization and process pH for production of recombinant human B-type natriuretic peptide under the cSAT scheme in Escherichia coli
Abstract Background Tag-based high-load expression in Escherichia coli can be accompanied by substantial drift of broth and intracellular pH, but mechanistic links between intracellular alkalinization, metabolism and process levers remain unclear. Results Using a cleavable self-aggregating tag…
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Artikel Jurnal
Systematic perturbation of cultures of K. phaffii by carbon co-feeding show gene signatures associated with production of recombinant proteins
Abstract Background Demand for recombinant proteins is rapidly growing, driven by their use as biotherapeutics, vaccine components, industrial enzymes, and food ingredients. The growing market requires novel strategies for increasing protein production in cellular hosts. Systems-level frameworks…
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Artikel Jurnal
Green metabolic engineering of Synechocystis sp. PCC 6803 for efficient production and sustainable extraction of β-cryptoxanthin and zeaxanthin
Abstract Background β-xanthophylls, such as zeaxanthin and β-cryptoxanthin, display significant bioactive properties including anti-oxidant, anti-inflammation and anti-cancer activities. These properties spur their widespread use in food, animal feed, cosmetic and pharmaceutic sectors. However…
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Artikel Jurnal
Functional characterization of a novel β-glucosidase converting trillin to diosgenin from Enterococcus faecalis
Abstract Background Diosgenin, known as “medicinal gold”, is the main precursor for the synthesis of steroid hormone drugs. Currently, diosgenin is primarily produced industrially via the extraction of plant-derived steroidal saponins followed by acid hydrolysis, which generates substantial acidic…
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Artikel Jurnal
Functional characteristics and distribution of microorganisms in saline and alkaline soils and their probiotic effects: a review
Abstract With more than 833 million hectares of saline soils worldwide, which are found primarily in arid and semiarid regions, soil salinisation poses a serious threat to global food security and agricultural development. Therefore, a variety of saline soil improvement strategies, including…
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Artikel Jurnal
Antifungal activity of oily core PEGylated PLGA nanocapsules loaded with Penicillium oxalicum fungal extract: in vitro, in vivo, and in silico study
Abstract The rise of difficult-to-treat fungal infections necessitates novel therapeutic strategies. In this study, endophytic fungi were isolated from Acalypha hispida leaves and molecularly identified as Penicillium oxalicum via 18S rRNA sequencing. LC–MS/MS analysis of the fungal extract…
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Artikel Jurnal
Enhanced L-tyrosine production in Bacillus amyloliquefaciens through alleviation of feedback Inhibition and coordinated pathway expression
Abstract Background L-tyrosine is a commercially significant compound with broad applications in the food and pharmaceutical industries. The development of efficient microbial cell factories for its production is of great interest. This study describes the engineering of the food-safe Bacillus…
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Artikel Jurnal
Heterotrophic fermentation of a robust human defensin in Chlamydomonas reinhardtii provides a stable and potent antibacterial
Abstract Background The growing threat of antibiotic resistance underscores the urgent need for novel therapeutic agents. Antimicrobial peptides (AMPs), such as human defensins, are promising candidates due to their broad-spectrum activities, but their widespread application is severely hindered by…
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Artikel Jurnal
Enhanced rhamnolipid production in Pseudomonas aeruginosa 8D by systematic genetic engineering
Abstract Rhamnolipids are environmentally friendly biosurfactants primarily produced by Pseudomonas aeruginosa. The complex and tightly regulated network imposes limitations on rhamnolipid biosynthesis. Therefore, rational genetic engineering of P. aeruginosa to optimize metabolic flux holds…
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Artikel Jurnal
Metabolic engineering for improved heterologous pinene production in the chloroplast of Phaeodactylum tricornutum
Abstract Background The marine diatom Phaeodactylum tricornutum is a promising platform for the sustainable production of terpenoids. This is due to its robust photosynthetic growth and natural accumulation of terpenoids, mainly including photosynthetic pigments. P. tricornutum harbors the…
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Artikel Jurnal
sRNA196 promotes Pseudomonas aeruginosa virulence by regulating target genes PA4398, ZapE and PhoB
Abstract Bacterial sRNAs is important mechanisms regulating bacterial pathogenesis. In this study, We screened a novel sRNA located at PA5007 closely associated with Pseudomonas aeruginosa infection by transcriptome sequencing196. And using transcriptome sequencing as well as public databases, the…
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Artikel Jurnal
Gold nanoparticle-mediated metabolic engineering in Trichoderma longibrachiatum MD33 unveils a hybrid terpenoid-alkaloid pathway for enhanced dendrobine biosynthesis
Abstract Background Dendrobine, a neuroprotective and anticancer sesquiterpenic alkaloid, is primarily sourced from endangered Dendrobium orchids, posing sustainability challenges to its production. Endophytic fungi, such as Trichoderma longibrachiatum MD33, offer an alternative; however…
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Artikel Jurnal
Using dual chamber microbial fuel cells for coupled microplastic biodegradation and bioelectricity production: assessing the effect of substrate
Abstract Background Use of plastics has increased manifold over the last 50 years. Regrettably, their fragmentation into microplastics (MPs) (≤ 5 mm) created environmental and human health concerns. Traditional methods of MPs removal are limited by high energy requirements, high costs, and the use…
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Artikel Jurnal
Imperatives for anaerobic high cell density culturing by denitrification: technical and physiological perspectives
Abstract Background Single-cell protein (SCP) is gaining attention as a source of food and feed, offering a lower environmental footprint than traditional agriculture. Efficient SCP production requires high cell density culturing (HCDC; >20 g cell dry weight L− 1), but O2 supply can become limiting…
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Artikel Jurnal
Staphylococcus aureus’s golden-yellow pigment staphyloxanthin: production enhancement, analytical characterization, and biological attributes
Abstract Staphyloxanthin (STX), an apocarotenoid golden yellow pigment produced by Staphylococcus aureus isolates, is endowed with potent antioxidant capacity. It plays a crucial role in combating reactive oxygen species (ROS), exhibiting considerable application prospects. This review provides a…
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Artikel Jurnal
Adaptive responses of genome-reduced Bacillus subtilis during enzyme secretion in a breathing vessel culture
Abstract Background The utilisation of microbes for the production of enzymes and pharmaceutical proteins is an important step towards a sustainable future. However, the energy requirement for agitation, aeration and cooling during industrial fermentation processes is substantial. To address this…
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Artikel Jurnal
Enhancing the bioconversion of phytosterols to 22-hydroxy-23,24- bisnorchol-4-ene-3-one in Mycobacterium neoaurum ZS-15 through genetic modification of kstD1 and wecA
Abstract Background C22 steroidal intermediates, including 22‑hydroxy‑23,24‑bisnorchol‑4‑ene‑3‑one (4‑HBC) and its Δ1‑dehydrogenated derivative 1,4‑HBC serve as key precursors for synthesizing progestational and adrenocortical hormones. However, the accumulation of multiple products coupled with…
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Artikel Jurnal
Metabolite profiling of Bacillus velezensis DM extract linked to the rhizosphere of Datura metel L. and dermatoprotective potential of an isolated glycoglycerolipid
Abstract Background Rhizosphere bacterial metabolites play a pivotal role in drug discovery by producing diverse bioactive compounds with cosmeceuticals and pharmaceuticals applications, offering eco-friendly and potent alternatives for skin care, wound healing, and therapeutic formulations. This…
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Artikel Jurnal
Directed screening and spatial coupling of farnesyl diphosphate synthase for enhancing menaquinone-7 production in Bacillus subtilis
Abstract Background Menaquinone-7 (MK-7), a highly bioactive form of vitamin K₂ with a long half-life, plays pivotal roles in preventing osteoporosis and cardiovascular diseases. A metabolically balanced MK-7-producing strain, Bacillus subtilis BS016, has been engineered. However, its biosynthetic…
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Artikel Jurnal
Redirecting linear hydrocarbon metabolism toward polyhydroxyalkanoate biosynthesis
Abstract This study explores how Pseudomonas putida EM2-4 metabolizes linear alkanes after acquiring an integrative and conjugative element (ICE) encoding a novel alkane monooxygenase (AlkB). This AlkB enzyme is a fusion of a hydroxylase and a fatty acid desaturase, exhibiting a narrow substrate…
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