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Engineering in Life Sciences
Scopus
ISSN 1618-2863 · Maximum Academic Press · 279 artikel di indeks
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Artikel Jurnal
Design and Characterization of a 3D‐Printable Membrane Aeration Module for Small‐Scale Bioprocess Prototyping
ABSTRACT Oxygen transfer is a critical design parameter in laboratory‐scale bioprocess systems used for prototyping, process development, and scale‐down studies of mammalian cell cultures, particularly when cultivating shear‐sensitive mammalian cells. In this work, we present the design and…
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Artikel Jurnal
Molecular Pharming: Advances, Applications, and Future Prospects in Biotechnology and Medicine
ABSTRACT Genetically engineered plants incorporate the use of a novel bioreactor known as molecular pharming, which has a transformative view on the pharmaceutical industry. The technique enables mass production, at a low cost, and reproducibly of a large number of different protein‐based drugs…
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Artikel Jurnal
Optimized Bioconversion of Naringenin to Hesperetin in Escherichia coli With Halide Methyltransferase‐Mediated S‐Adenosylmethionine Regeneration
ABSTRACT Hesperetin is a bioactive flavonoid with potential applications in pharmaceuticals and nutraceuticals, yet its low natural abundance limits commercial use. In this study, a two‐step whole‐cell bioconversion process was developed for the microbial production of hesperetin from naringenin in…
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Artikel Jurnal
Investigation of Methacryloyl‐Modified Gelatin‐Based Hydrogels for Inkjet‐Printed Biosensors and Their Adherence to Polyethylene Terephthalate and Oriented Polypropylene Substrates
ABSTRACT The COVID‐19 pandemic has highlighted the need for rapid, simple, and cost‐effective point‐of‐care testing (POCT) methods for pathogen detection. Hydrogel‐based biosensing has emerged as an increasingly popular approach, offering advantages such as reagent storage and multiplexing…
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Artikel Jurnal
Careful CO Addition Enhances Autotrophic d‐Lactate Formation With Engineered Acetobacterium woodii
ABSTRACT The acetogen A. woodii efficiently converts CO2 and H2 to acetate. Metabolic engineering enabled the autotrophic production of non‐native products, for example, d‐lactate from CO2 by overexpression of d‐lactate dehydrogenase from Leuconostoc mesenteroides and knockout of the native lactate…
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Artikel Jurnal
Continuous Enzymatic Peracids Synthesis in Pickering Emulsions: Influence of Nanoparticles Modification, Aqueous Phase Composition and Operational Parameters
ABSTRACT The lipase‐catalyzed oxidative functionalization of alkenes in Pickering emulsions (PE) offers a green and efficient alternative to conventional processes involving hazardous oxidants. Other investigated reaction media used for this alternative green pathway still suffer from enzyme…
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Artikel Jurnal
Filament Extrusion‐Based Conductive TPU Composite Scaffolds Enable Superior Neuronal Growth and Synaptic Maturation In Vitro
ABSTRACT Fused filament fabrication (FFF) three‐dimensional (3D) printing technologies offer new opportunities for fabricating customizable, low‐cost platforms for tissue engineering applications. Here, we developed and characterized 3D‐printed scaffolds using conductive thermoplastic polyurethane…
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Artikel Jurnal
Process Systems Engineering in Precision Medicine: Opportunities in Autologous CAR‐T Therapy
ABSTRACT Autologous chimeric antigen receptor (CAR)‐T therapies have given hope to many cancer patients whose other lines of treatment have failed. Unfortunately, limited manufacturing capability has resulted in many patients dying while on a waitlist. Similarly, since clinical trial treatments are…
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Artikel Jurnal
Synergistic Combination of Additive One‐ and Two‐Photon Polymerization Printing Methods to Fabricate 3D Microstructured Perfusable Angiogenesis–on–a–Chip Systems
ABSTRACT Tissue engineering, and in particular the development of organ‐on‐a‐chip (OOC) models, holds significant promise for advancing personalized medicine and reducing the use of animal models. The integration of microfluidics and advanced biomaterials in OOC systems provides controlled…
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Artikel Jurnal
What Is a Biosensor?—A Terminological Guide From Biomolecular Recognition to Bioindicators
ABSTRACT Biosensors are an integral part of modern medicine, are used in basic research, and are increasingly used by consumers as point‐of‐care and wearable devices. Meanwhile, the underlying technological approaches are rapidly expanding, including spectroscopic sensing, artificial bioreceptors…
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Artikel Jurnal
Evaluation of 3D‐Printed Resin Materials for Use in Microbial Bioprocess Applications: Suitability and Autoclavability
ABSTRACT Three‐dimensional (3D) printing technologies are continually advancing, significantly broadening their application scope. By employing fused deposition modeling (FDM) to create iterative prototypes and stereolithography (SLA) for crafting functional components, these additive manufacturing…
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Artikel Jurnal
The Impact of Fungal Developmental Structures on Mechanical Properties of Mycelial Materials
ABSTRACT This study explores how suppressing asexual development in Aspergillus nidulans enhances the mechanical properties of mycelial materials. Using four aconidial mutants (∆brlA, ∆flbA, ∆fluG, and fadAG42R) lacking asexual development and a control strain (A28) that undergoes typical asexual…
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Artikel Jurnal
Exploring Ectoine Production From Methanol, Formate, and Electrochemically Produced Formate by Methyloligella halotolerans
ABSTRACT Microbial synthesis using renewable C1‐carbon sources like electrochemically produced formate (e‐formate) represents a promising approach for climate‐neutral chemical production. This study investigates formate utilization for ectoine biosynthesis by the halophilic methylotroph…
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Artikel Jurnal
Less Genome, More Gain: Genome Reduction Enhances Transaminase‐Producing E. coli in a Scale‐Down Bioreactor
ABSTRACT In large‐scale bioprocesses, mixing limitations and design constraints cause the onset of heterogeneous environments, subjecting the cells to continuously changing external conditions, often reducing their performance compared to laboratory conditions. This study evaluated the performance…
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Artikel Jurnal
Progressive Cavity Pumps—A Comparison of New Technology for Gradient Bioprinting to Existing Extrusion Methods
ABSTRACT Extrusion‐based Bioprinting is a key technology in biofabrication, yet the choice of extrusion method is often limited to established techniques built into most bioprinters, limiting the print fidelity and more demanding applications like printing material gradients. In this technical…
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Artikel Jurnal
Reconstruction of de novo verazine biosynthesis in Saccharomyces cerevisiae and computational analysis of cholesterol-binding geometry for 22R hydroxylation by VcCYP90B27 to dissect the cyclopamine biosynthetic pathway
Cyclopamine, a typical isosteroidal alkaloid identified as a hedgehog pathway inhibitor, serves as a crucial molecular scaffold for the semi-synthesis of drugs treating nevoid basal cell carcinoma. Verazine is a key intermediate in the biosynthetic pathway of cyclopamine. In this study, we…
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Artikel Jurnal
Integrative modeling of cold and light signaling in tomato: unraveling the transcriptional network governing cold acclimation
The integration of light and cold signaling is critical for cold acclimation in plants, but the regulatory complexity of the underlying transcriptional network remains poorly understood. To systematically dissect this interplay in tomato (Solanum lycopersicum), we constructed a computational model…
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Artikel Jurnal
Engineering 3D adipose cell models by support bath bioprinting of mature adipocytes in gellan gum hydrogels
Mature adipocytes (ACs) are essential for modeling human adipose tissue physiology in vitro, yet their large size, buoyancy, and shear sensitivity make them challenging to incorporate into engineered three-dimensional (3D) constructs. This study introduces an agarose support-bath (SB) extrusion…
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Artikel Jurnal
Regulatory mechanisms and multidimensional applications of blueberry anthocyanins: a review of current gaps and future directions
Blueberries are among the richest fruit sources of anthocyanins, which contribute significantly to fruit quality, flavor, and plant physiology. Anthocyanins also exhibit various health-promoting effects, making them valuable in the food, pharmaceutical, and cosmetic industries. Although the…
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Artikel Jurnal
Synthetic promoters developed through a site-specific integration system enhance antibody expression in fed-batch culture
Constructing expression cell lines using site-specific integration (SSI) technology has become a prominent focus in cell line development (CLD) because of its potential to minimize clonal variation. However, this technology has not yet been widely adopted in commercial biopharmaceutical…