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Journal of Biological Engineering
Scopus
ISSN 1754-1611 · BMC · 772 articles in the index
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Journal Article
Smartphone-based multispectral autofluorescence analysis of bacteria mixtures of staphylococci using convolutional neural network
Abstract Despite the numerous merits of bioreceptor-based biosensors for detecting bacteria, they often fail to identify complex bacterial mixtures, such as those encountered in microbiome analysis, especially when the bacterial mixtures belong to the same genus. Additionally, their procedure…
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Journal Article
Comparison of different decellularization protocols for porcine centrum tendineum diaphragmatis and diaphragmatic muscle – a base for effective recellularization
Abstract Diaphragmatic dysfunction results from a variety of diseases or post-surgical conditions, leading to impaired lung function and high morbidity and mortality. Current repair materials are limited by poor biocompatibility, functional incompatibility and immune reactions. Tissue engineering…
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Journal Article
Effect of high hydrostatic pressure on D-amino acid oxidase activity and stability
Abstract We report the effects of high hydrostatic pressure (HHP) on D-amino acid oxidase (DAAO) from pork kidney stability and activity. At 20 °C, the activity decreased with increasing pressure, but at 40 °C, it was not significantly affected because the increased catalytic rate compensated for…
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Journal Article
Water microdroplet platforms for sustainable, reagent-free viral disinfection
Abstract Background The repeated emergence of global pandemics has highlighted the urgent need for safe, sustainable, and effective disinfection platforms that eliminate viruses without producing toxic by-products or causing surface damage associated with conventional methods such as ultraviolet…
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Journal Article
Curcumin-loaded silk fibroin scaffold promotes cartilage regeneration by inhibiting angiogenesis via Drp1/ROS-mediated mitochondrial regulation
Abstract Articular cartilage injury often leads to vascular endothelial cell (VEC) infiltration, disrupting the microenvironment between cartilage and subchondral bone, thereby compromising cartilage repair quality. Curcumin (Cur) is a natural polyphenol with anti-inflammatory and anti-angiogenic…
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Journal Article
Effect of HIF-1α inhibitor LW6 on chondrogenic properties of mesenchymal stromal cells and chondrocytes in cell sheets under physioxia
Abstract Background Hypoxia-inducible factor 1 (HIF-1) plays a central role in cartilage homeostasis, and its modulation may influence the performance of cell-based therapies. LW6, an inhibitor of HIF-1α and a potential anti-cancer therapeutic, has not been previously evaluated in cartilage…
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Journal Article
Enhancing gene therapy vectors manufacturing: CEBPA as a master regulator of rAAV biogenesis
Abstract Background Recombinant adeno-associated viruses (rAAVs) are the vectors of choice for gene therapy applications due to their favorable safety and efficacy profiles. However, current production platforms—particularly transient transfection-based HEK293 systems—have scalability challenges…
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Journal Article
Smart biomaterials for cardiovascular, bone, and skin tissue engineering: mechanisms, applications, and future prospects
Abstract Smart biomaterials are engineered to dynamically respond to external stimuli, such as pH, temperature, light, and magnetic fields, enabling on-demand modifications in their physicochemical properties. The limited regenerative capacity of tissues and organs has driven the development of…
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Journal Article
Thiol release improvement in Saccharomyces cerevisiae oenological strains by CRISPR/Cas9-based IRC7 molecular cisgenesis
Abstract Background Polyfunctional thiols are essential contributors to the aromatic profile of varietal white wines like Sauvignon Blanc. These compounds are released during fermentation by Saccharomyces cerevisiae cells from grape-derived precursors, with the carbon-sulfur β-lyase encoded by the…
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Journal Article
Metabolic engineering of Escherichia coli BW25113 for the production of Vitamin K2 based on CRISPR/Cas9 mediated gene knockout and metabolic pathway modification
Abstract Background Vitamin K2 (VK2), as a derivative of the menaquinone family, plays an important role in the prevention of osteoporosis and cardiovascular calcification. The realization of the industrialization of VK2 and the reduction of its production cost have become the focus of attention…
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Journal Article
Anti-CD31 antibody preconditioning for enhancement of endothelial cell capture and vascularization: a novel strategy for bioengineering lung scaffolds
Abstract Recellularization of acellular lung scaffolds is a promising technique for the generation of bioengineered lungs. However, at the present time, pre-clinical evaluation of bioengineered lungs shows graft failure likely due to thrombus formation and bleeding from bronchi. This is believed to…
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Journal Article
Construction of Escherichia coli L-isoleucine cell factories based on propionate pathway
Abstract Isoleucine, an essential branched-chain amino acid with broad applications in food, pharmaceuticals, and feed, is predominantly produced via the microbial threonine pathway, which suffers from catalytic complexity and regulatory inefficiency. The propionate pathway offers a streamlined…
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Journal Article
Unveiling the mechanism of abdominal and transcranial ultrasound stimulation against DSS-induced colitis based on proteomic analysis
Abstract Inflammatory bowel disease (IBD), particularly ulcerative colitis (UC), is increasingly recognized for its systemic effects, including neuroinflammation and cognitive deficits mediated through the gut-brain axis. This study investigates the potential mechanisms for treating UC with…
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Journal Article
An innovative cholesteric liquid crystal biosensor enabling high-contrast colorimetric detection and haze-based quantitation
Abstract Background A cholesteric liquid crystal (CLC) system comprising the calamitic LC 8CB (4-octyl-4’-cyanobiphenyl) doped with the chiral agent R5011 was applied as a biosensing medium. Results By exploiting the pronounced light-scattering behavior at the smectic-to-chiral nematic transition…
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Journal Article
Co-expression of holin gene improves heterologous cellulase secretion and surface display by lactic acid bacteria Lactococcus cremoris
Abstract Background Genetically modified Lactococcus cremoris strains producing heterologous cellulases emerge as promising candidates for cost-effective bioprocessing of plant biowaste into high-value organic compounds. A key challenge in enabling efficient cellulose degradation by L. cremoris…
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Journal Article
Real time monitoring of hydrogenotrophic methanogenesis under deep saline aquifers conditions
Abstract To investigate the microbial response to deep underground gas injection, specifically CO2 and H2, a new optically transparent high-pressure reactor was developed to monitor autotrophic microbial growth via in situ and ex situ characterization techniques. The main advantages rely on…
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Journal Article
Preserving integrity: innovative in vitro methods for extracellular matrix decellularization and collagen purification
Abstract Background In tissue engineering and cell therapy development, synthetic biomaterials are frequently supplemented with collagen or other extracellular matrix (ECM) components to enhance biocompatibility. To support these applications, novel methods for collagen purification and ECM…
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Journal Article
Bioreactor expansion of human neural progenitor cells for exosome scalable production and miRNA-engineering
Abstract Upscaling protocols to produce exosomes from human neural precursor cells (NPCs) are crucial for enabling broader therapeutic applications for neurodegenerative diseases with associated inflammation. Exosomes are small extracellular vesicles measuring between 30 and 150 nm in diameter that…
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Journal Article
Optimization of serum-free medium supplemented with microalgal extract using response surface methodology for human fibroblast culture
Abstract Background Animal cell culture has become an essential tool in biomedical research and industrial vaccine and biopharmaceutical development. Fetal bovine serum (FBS) is widely used as a culture supplement because of its rich composition of nutrients and growth factors. However, concerns…
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Journal Article
A detoxified exosome–ECM hydrogel depot for localized and sustained chemotherapy in prostate cancer
Abstract Advanced prostate cancer often necessitates chemotherapy, yet conventional agents such as doxorubicin (DOX) suffer from poor tumor specificity, systemic toxicity, and acquired resistance. Here, we report a biomimetic, injectable hydrogel depot that integrates enzyme-detoxified tumor…