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BioTechniques
Scopus
ISSN 1940-9818 · Taylor & Francis Group · 5,998 articles in the index
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Journal Article
Optimization of agarose–gelatin double embedding to minimize sectioning artifacts in multicellular spheroids
Processing methods that do not induce shape distortion are essential for the analysis of tissue morphology. Although the use of agarose–gelatin double embedding to reduce shape distortion has been suggested, the effect of matrix concentration has not been addressed. Therefore, combinations of…
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Journal Article
3in1: an ultra-fast phage display biopanning method overcoming the biopanning bias and yielding more diverse binders
Phage display combined with biopanning is a powerful method for the discovery of therapeutic antibodies. Classic biopanning is lengthy and labor-intensive, requiring at least five days of work. The biopanning process is plagued by a bias toward binders with propagation advantages, causing a loss in…
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Journal Article
A robust and sensitive method for detecting subtle structural differences in bovine serum albumin
Bovine serum albumin (BSA) exhibits lot-to-lot variability, partly due to differences in fatty acid content. In this study, the structural properties of two BSA lots—fatty acid–bound and -free—were compared using electrophoretic, chromatographic, and spectroscopic techniques. No apparent…
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Journal Article
Self-circularized Overhang-based Unrecombined Products (SOUP): a rapid in vitro method for generating backbone-free expression cassettes
Plasmid DNA remains the standard tool for mammalian transfection but carries bacterial backbone sequences that can reduce expression efficiency and raise biosafety concerns. Minicircle DNA eliminates these elements and improves expression but requires bacterial recombination systems and multi-day…
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Journal Article
High-resolution molecular typing of vancomycin-resistant Enterococcus faecium from Romania and Bavaria: combining enhanced DNA microarray and next generation sequencing
Antimicrobial resistance poses a significant challenge for infection control, requiring the development of accurate and high-throughput diagnostic techniques. We expanded and optimized an existing DNA microarray platform for the molecular characterization of vancomycin-resistant Enterococcus (VRE)…
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Journal Article
Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides
Introduction: Quantitative assessment of lung tissue architecture is essential for evaluating disease progression in experimental models of acute lung injury (ALI). However, conventional methods for measuring alveolar septum thickness rely on manual procedures that are time-consuming…
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Journal Article
Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line
Cell line stability of mammalian cell lines is an important concern for manufacturing of biopharmaceuticals. Characterization of the master cell bank (MCB) for monoclonal antibody A (Mab A) via multiplex fluorescence in situ hybridization (M-FISH) analysis identified two distinct karyotypic cell…
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Journal Article
A refined qPCR duplexing method for the molecular sex determination of sturgeon (Acipenser genus), providing a validating, endogenous control
Previous molecular methods for sex determination of sturgeon of the Acipenser genus have been repeatedly validated for females, but have largely relied on suboptimal solutions to compensate for the lack of a confirmed control for males. By combining sturgeon cytochrome b (cyt b) gene amplification…
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Journal Article
Recent advances in deep learning for biological microscopy image analysis beyond segmentation
In the past decade, we have witnessed an unprecedented growth of artificial intelligence (AI) techniques for high-resolution microscopy images. More importantly, we are observing a paradigm evolution of these deep learning (DL) methods, from a post-hoc analysis tool to nowadays an essential…
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Journal Article
Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers
Long interspersed nuclear element-1 (LINE-1 or L1) is the only autonomously active retrotransposon in the human genome and produces both sense and antisense transcripts from its 5′ untranslated region (5′UTR) of L1Hs, a human-specific L1 subfamily. Among these, ORF0 is an antisense…
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Journal Article
Detection of antisense oligonucleotides from biological samples by ligase detection reaction using T4 RNA ligase 2
This study developed a detection method for antisense oligonucleotides (ASOs) by ligase detection reaction (LDR) using T4 RNA ligase 2 (T4Rnl2). The LDR was applied to detect a nusinersen-based ASO, an 18-mer 2’-O-methoxyethyl-modifiedoligonucleotide with phosphorothioate linkages. The method…
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Journal Article
A QC-guided framework for performance assessment in plate-based RNAi screening with fluorescence and metabolic readouts
RNA interference (RNAi) screening is widely used for systematic gene perturbation, but reproducible readouts depend on careful assay design and consistent performance. Here, we describe a plate-based small interfering RNA (siRNA) screening workflow that combines repeated green fluorescent protein…
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Journal Article
A PCR method to detect the mouse major histocompatibility complex H2-Ld gene that confers resistance to Toxoplasma encephalitis
In laboratory mice, the H2-Ld gene confers resistance to encephalitis upon infection with Toxoplasma gondii, a foodborne parasite capable of infecting all endotherm animals tested. H2-Ld is carried by inbred strains with major histocompatibility complex (MHC) haplotypes H-2a (e.g., A/J) and H-2d…
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Journal Article
In vivo RNA targeting in the nematode Caenorhabditis elegans using exogenous catalytic DNA
Catalytic DNA molecules (DNAzymes) offer a programmable means to manipulate RNA without genomic modification. Although DNAzymes have been thoroughly characterized in culture and in various animals, their utility in the prevalent model system, Caenorhabditis elegans, has not been explored. Here, we…
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Journal Article
A simple and rapid agarose gel electrophoresis method to assess CpG methylation of DNA
The presence of 5-methylcytosine at CpG sites in mammalian DNA plays a significant role in various biological processes, both normal and aberrant. Similarly, CpG site methylation status of therapeutic DNAs can affect activity and therapeutic efficacy, as is the case for ADI-100, an immune…
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Journal Article
Quantitative analysis of oligonucleotide delivery into isolated mitochondria: a proof-of-concept method
Mitochondria, with their own DNA, Represent a potential target for nucleic acid-based precision therapies. However, effective delivery of therapeutic oligonucleotides remains challenging due to the dual mitochondrial membranes and the localization of mitochondrial DNA within nucleoid complexes in…
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Journal Article
Generation and validation of C-terminal LRP8 antibodies for detecting processed intracellular fragments
Introduction: Low-density lipoprotein receptor-related protein 8 (LRP8) is a neuronal receptor for apolipoprotein E and Reelin, two ligands critically involved in Alzheimer’s disease (AD), neuronal migration, and memory. Because LRP8 is highly expressed in neurons, interacts with amyloid precursor…
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