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Microbiology Indonesia
ISSN 1978-3477 · Indonesian Society for Microbiology · 429 artikel di indeks
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Artikel Jurnal
Design of Adenovirus 5 Vector with Adenovirus 26 Hexon Hypervariable Region Sequence using In Silico Approach
Adenovirus type 5 (Ad5) is one of the vaccine vectors, including the COVID-19 vaccine. Pre-existing immunity to Ad5 may suppress the immunogenicity and efficacy of adenovirus vectored vaccine. The neutralizing antibodies are directed specifically toward seven hypervariable regions (HVR) of hexon…
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Artikel Jurnal
Multidrug Resistance and Extensively Drug-Resistance in Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus haemolyticus
Antimicrobial resistance in bacteria has become a leading global public health issue. Staphylococcus sp. has an efficient mechanism to deal with antimicrobial agents that make them hard to treat in hospital-acquired and community-acquired infections. This study was conducted due to limited data…
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Artikel Jurnal
The qPCR Assay for Detecting The Presence and Relative Abundance of Pseudomonas aerugionosa and Antibiotic Resistance Gene aadA2 in Hospital Wastewater of National Reference Hospital Dr. Cipto Mangunkusumo (RSCM)
Antimicrobial resistance is one of the top 10 global health threats. The hospital wastewater (HWW) potentially becomes the reservoir and dissemination of antibiotic resistance gene (ARG) and bacterial pathogens. In Indonesia, the protocol to monitor the ARGs form HWW has not been established. This…
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Artikel Jurnal
Rapid Detection of Foodborne Pathogen Bacteria Vibrio parahaemolyticus in Seafood Using Gene ToxR with Real-Time Polymerase Chain Reaction Method
Cases of food poisoning often occur due to food contamination caused by pathogenic bacteria. One of the pathogenic bacteria is Vibrio parahaemolyticus which is found in seafood. Thus, a fast, accurate and specific detection method is needed. The purpose of this study was to quickly detect Vibrio…
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Artikel Jurnal
Effect of Cocoa Bean Fermentation Using Lactic Acid Bacteria and Yeast Starters on Flavonoid Formation and Antioxidant Activity
This study investigates the effect of fermentation using lactic acid bacteria and yeast as starters on the formation of flavonoid compounds and the antioxidant activity of cacao beans. The fermentation process were divided into 4 groups: F1: spontaneous fermentation, F2: fermentation using Lactic…
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Artikel Jurnal
Endophytic Bacteria of Pemphis acidula on Karst Ecosystem of Gorontalo, Indonesia
Wild plant Pemphis acidula in coastal habitats is often associated with microbes on their root, stem, or leaf. The objective of the study was to reveal the endophytic bacteria associated with Pemphis acidula in the coastal area of Gorontalo, the study antibacterial activity of endophytic bacteria…
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Artikel Jurnal
Combination of Pseudomonas fluorescens and Liquid Organic Fertilizer on Growth and Production of Peanut (Arachis hypogaea L.)
Excessive and continuous use of synthetic chemicals results in a decrease in soil fertility, thereby reducing peanut production. Minimizing the use of synthetic chemicals can be done through the application of liquid organic fertilizers. This study aims to examine the use of Pseudomonas fluorescens…
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Artikel Jurnal
The Effect of Pili Protein of Klebsiella pneumoniae 65,5 kDa on Enhanced IFN- Gamma Levels in Mice Liver
Klebsiella pneumoniae develops antibiotic resistance by producing enzymes such as Extended-Spectrum Beta-Lactamase and Carbapenemase. Antibiotic resistance causes K. pneumoniae to have less antibiotic activity and more virulence factors. Capsule polysaccharides, lipopolysaccharide, Outer Membrane…
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Artikel Jurnal
Anti Fungal Activity of Chitinolytic Bacteria Lysinibacillus fusiformis and Brevibacillus reuszeri Against The Fungal Pathogens Rhizoctonia solani and Fusarium oxysporum
Abstract. Chitinolytic bacteria can produce chitinase, reported as a biocontrol agent against plants. This research aims to see chitinolytic activity in inhibiting the growth of Rhizoctonia solani and Fusarium oxysporum. Anti fungal testing in dual culture test by growing each of the chitinolytic…
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Artikel Jurnal
Antibacterial and Antioxidant Activities of Ginger Essential Oils
Ginger is a rhizomatous perennial herb that grows abundantly in tropical areas. It has been used around the world as a spice, flavoring agent, and ingredient in traditional medicine. Ginger essential oils (GEOs) are derivatives of ginger that can be found in various products used in daily life…
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Artikel Jurnal
Lipase Activity Enhancement of KC4J Mutant from Oil Palm Waste Using Response Surface Method and Characterization.
Lipase has an important role in industry. The KC4J mutated isolates from oil palm waste had 100% similarity to Aspergillus fumigatus strain RA204, a fungus known produce lipase. The study aimed to increase lipase activity of KC4J mutant through media optimization using Response Surface Methodology…
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Artikel Jurnal
Antibacterial Activity of Ethanol Extract of Portulaca oleracea L. Herb from Various Extraction Methods Against Salmonella typhimurium
Salmonella typhimurium bacteria could cause gastroenteritis and its growth could be controlled by the active compounds from natural products, which is Portulaca oleracea L. herb. Portulaca oleracea contained tannin, saponins and flavonoids compounds which had different characteristics towards…
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Artikel Jurnal
IgG subclasses identification of immunized mice sera with Dengue tetravalent DNA vaccine based on prM-E genes
Background: Dengue fever is still a serious health problem in the world. DENV consists of 11 kb of single positive-stranded RNA encoding three structural proteins and seven non-structural proteins. PrM and E proteins are the main targets of the antibody response that rich of epitopes and able to…
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Artikel Jurnal
Bacterial Community Profiles in Tapai Singkong: a Traditional IndonesianFermented Food from Cassava Tubers
Tapai singkong is one of the popular fermented foods in Indonesia, which is processed from cassava tubers (Manihot utilissima ). The bacteria present during the fermentation process de Manihot utilissima determines the quality of Tapaisingkong. However, information about the bacteria of Tapai…
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Artikel Jurnal
Optimization of Rhizopus spp. Production as Mycoprotein using Soymilk Media
Mycoprotein is food with high protein content, fiber, and low in cholesterol made from fungal mycelium. In this research, mycoprotein was produced by spp. isolated fr Rhizopus om tempeh with soymilk as growth media. This research aims to determine the best strain of spp. and op Rhizopus timum…
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Artikel Jurnal
Pretreated Sugarcane Bagasse Result in more Efficient Degradation by Streptomyces sp S2
Streptomyces genera plays an important role in lignocellulose degradation. Many research found that Streptomyces have cellulolytic and ligninolytic enzymes that are sufficient to degrade lignocellulosic materials. However, a minimum lignocellulosic material condition that can efficiently be…
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Artikel Jurnal
Relationship and structural diversity of bacterial manganese superoxide dismutases and the strategy for its application in therapy and cosmetics
Manganese superoxide dismutase (MnSOD) from bacteria shares high amino acid sequence homology and nearly identical structure. Despite of that, their characteristics are diverse, which likely due to their bacterial origin and adaptation to the environment. Most importantly, their structural…
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Artikel Jurnal Teks lengkap
Thermostable alkaline protease activity from Aspergillus flavus DUCC- K225 and its compatibility to local detergents
Protease is the most important enzyme in many industries, including detergent. Aspergillus flavus DUCC-K225 is a thermotolerant indigenous molds isolated from Madura island which is potential in producing thermostable alkaline protease enzymes. The enzyme produced by submerged culture on modified…
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Artikel Jurnal
Exploration of lignocellulolytic microbes in oil palm rhizosphere on peat soils and their respiration activities
Microbial respiration in peatlands plays a role in contributing CO2 emissions. Studies of microbial exploration and respiration on peat soils in oil palm plantations have not been widely reported. This study aims to explore lignocellulolytic microbes found in peat soils in compared with mineral…