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Microorganisms
Scopus
ISSN 2076-2607 · MDPI AG · 18.813 artikel di indeks
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Recombinant Cytosolic Truncations of Histidine Kinases Retain Function for Targeted In Vitro Investigations
Histidine kinases are an integral component of bacterial two-component systems (TCSs), playing a pivotal role in signal transduction pathways, resulting in both resistance and virulence. However, their inherent membrane-bound nature often results in poor solubility, making them difficult to isolate…
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Microbial Succession on Honey Bee Body Surfaces Reflects Behavioral Maturation
Behavioral maturation is essential for the proper functioning of honey bee societies and is regulated by multiple factors such as juvenile hormone (JH) and nutritional deficiency. Although recent studies have shown that surface-associated microbiota in insects can modulate host behavior, the…
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A Swiss Retrospective Case Series of Pediatric Primary Subacute Hematogenous Osteomyelitis
This study aimed to characterize the clinical spectrum and the bacteriological and microbiological etiology of pediatric primary subacute hematogenous osteomyelitis (PSAHO) and to evaluate a modern diagnostic approach for these infections. A single-center, 25-year retrospective review (2000–2025)…
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Interplay of Gastrointestinal Parasites, Micronutrient Deficiencies, and Anemia in Children from the Bolivian Highlands
Children living in resource-limited regions with inadequate environmental sanitation, such as the Bolivian highlands, are affected by parasitic infections that may compromise nutritional status. Objective: This study aimed to assess the prevalence of intestinal parasitic infections and their…
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Intrinsic Acidity of N-Acetylcysteine Mediates Enhanced Inhibition of Klebsiella pneumoniae and Its Biofilms by Polymyxin B
This study investigated the combined antibacterial and anti-biofilm activity of polymyxin B (PB) with intrinsically acidic N-acetylcysteine (NAC) against Klebsiella pneumoniae. The minimum inhibitory concentrations (MICs) of PB, acidic NAC, and neutralized NAC against 34 K. pneumoniae strains were…
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Diet-Induced Modulation of Gut Microbiota Affects Meat Quality in Grass Carp (Ctenopharyngodon idellus)
Understanding meat quality and gut microbiota hold great potential for promoting healthy and sustainable fish production, while also contributing to fisheries management and conservation. However, differences in meat quality and the diversity, structure, and function of gut microbiota among fish…
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Occurrence and Genomic Characterization of ESBL-, AmpC-, and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolated from Surface Water in Southern Italy, 2023–2024
Antimicrobial resistance (AMR) is recognised as a major global public health threat, with the environment increasingly acknowledged as a key reservoir and dissemination pathway for resistant bacteria and resistance genes. In this study, 148 surface water samples were collected between 2023 and 2024…
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Genomic Characterization of Salmonella Isolates Causing Infections in Children with Sickle Cell Disease in Dakar, Senegal
Salmonella is a major bacterial pathogen in low-income countries, where it circulates among humans, animals, and the environment. Children with sickle cell disease (SCD) are particularly vulnerable to severe Salmonella infections. This study aimed to characterize Salmonella isolates causing…
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Decoding Virulence Mechanisms of Bacillus anthracis Using a Galleria mellonella Infection Model: Differential Host Response Profiles Elicited by AtxA and PlcR
A thorough understanding of the functions of virulence regulators in Bacillus anthracis evolution and host adaptation, particularly the systematic host responses they trigger, requires an efficient infection model capable of resolving subtle mechanisms. This study constructed a high-resolution host…
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Phenotypic and Molecular Characterization of ESBL/pAmpC-Associated Resistance in Poultry- and Hatchery-Derived Escherichia coli in Bosnia and Herzegovina
Antimicrobial resistance (AMR) in poultry-associated Escherichia coli (E. coli) is a persistent One Health concern, particularly when ESBL/pAmpC determinants co-occur with resistance to multiple antimicrobial classes. Between March and October 2024, we investigated commensal E. coli from three…
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Lipidome Disturbances of Vibrio alginolyticus Associated with Citral Exposure
Vibrio alginolyticus is an important antibiotic-resistant pathogen in aquaculture that can cause mortality in a wide range of aquatic animals and infect humans. It is urgently necessary to discover and develop effective antibiotic alternatives. Citral, a key antibacterial component of lemongrass…
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From Basics to Breakthroughs: A Review on the Evolution of Campylobacter spp. Culture Media
Since their recognition as human pathogens in the 1970s, Campylobacter spp. have posed persistent challenges to microbiologists due to their fastidious growth requirements and environmental sensitivity. The continuous refinement of selective and differential culture media has been crucial for…
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Effectiveness of COVID-19 Vaccines Against Hospitalization and Severe Disease in Children with Diabetes Mellitus During Pandemic and Post-Pandemic Eras
Pediatric patients with SARS-CoV-2 infection are at an increased risk of severe disease and adverse outcomes. Nevertheless, comprehensive data on COVID-19 vaccine effectiveness (VE) in children with diabetes during the post-pandemic period remain limited. This study assessed the VE against severe…
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Biosurfactant-Producing Bacteria Isolated from a Microbial Consortium Previously Subjected to Adaptive Laboratory Evolution in Oily Sludge
Microbial bioprospecting in contaminated environments is a promising strategy for identifying biosurfactant-producing bacteria; however, translating environmentally adapted strains into predictable cultivation processes remains challenging. In this study, a microbial consortium subjected to…
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Biodiversity and Biotechnological Potential of Dunaliella sp. Isolates from Kalloni Solar Saltworks (Lesvos, Greece)
Hypersaline solar saltworks represent unique ecological niches that harbor extremophilic microalgae with considerable biotechnological potential. Within these environments, members of the genus Dunaliella are particularly noteworthy due to their remarkable metabolic plasticity and ability to…
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In Vitro Evaluation of Ethanolic Medicinal Plant Extracts from the Aseer Region Against Selected Cultured Oral Bacterial Isolates from Healthy Volunteers
This work provides an in vitro assessment of the antibacterial efficacy of ethanolic extracts derived from four medicinal plants historically utilized in the Aseer region (Foeniculum vulgare, Solanum incanum, Forsskaolea tenacissima, and Abutilon pannosum) against cultured oral bacterial isolates…
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Sand Fly Fauna (Diptera: Psychodidae): Association Between Climatic Variables and Natural Leishmania Infection in Araçatuba, Brazil
Visceral leishmaniasis (VL) is a zoonosis of major public health importance. In urban areas, Lutzomyia longipalpis is the primary vector of Leishmania (L.) infantum. This study assessed the seasonality, spatiotemporal distribution, and climatic factors associated with L. longipalpis abundance in…
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Seasonal Variations in the Occurrence of SARS-CoV-2 RNA Recovered from Wastewater Treatment Facilities (WWTFs) Within South Africa
Several researchers have documented the occurrence of the unfamiliar severe acute respiratory syndrome coronavirus 2 ribonucleic acid (also known as SARS-CoV-2 RNA) in various raw wastewater (WW) samples analyzed globally. The efficiency of strategic WW-based epidemiology (WBE) approach as a timely…
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Rapid Detection of Vibrio vulnificus with Recombinase Polymerase Amplification Assays Based on Specific Sequence Tags of Core Genome
Vibrio vulnificus (V. vulnificus) is a motile, Gram-negative, opportunistic human pathogen capable of causing severe to life-threatening infections in individuals with predisposing conditions. It has the highest mortality rate among foodborne pathogens. Rapid and accurate detection of V. vulnificus…
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Unraveling the Coevolutionary Dynamics of Phage and Bacterial Protein Warfare Occurring in the Drains of Beef-Processing Plants
Phages, the most abundant entities on Earth, exhibit a complex interplay with bacteria, especially within environmental biofilms, resulting in an ecological arms race. This study investigates the interaction between phages and bacteria in the drains of beef-processing plants using high-throughput…
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