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Biotechnology Reports
Scopus
ISSN 2215-017X · Elsevier · 957 artikel di indeks
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Artikel Jurnal
Generation and characterization of a HEK293 cell line optimized for recombinant adeno-associated virus production
HEK293 is a preferred cellular platform to produce viral vectors including adeno-associated viruses (AAV). However, HEK293 cells were shown to be genomically unstable and many HEK293 cell lines having distinct genotypes and phenotypes have been reported. Here we generated a stable clonal cell line…
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Double-layer microencapsulation of Lacticaseibacillus rhamnosus using sodium alginate and nano-mucilages of Qodume Shirazi and Camelina sativa for probiotic goat milk dessert
This study is the first to utilize nano-scale Qodume Shirazi seed mucilage (QSSM) and Camelina sativa seed mucilage (CSSM), individually and in combination, as natural wall materials for microencapsulation of Lacticaseibacillus rhamnosus (LR). SEM analysis indicated smooth bead surfaces and…
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Artikel Jurnal
Development of anti-dengue virus D8 neutralizing monoclonal antibody production in Nicotiana benthamiana
Dengue is a life-threatening mosquito-borne viral disease ranging from mild symptoms to severe hemorrhagic fever. In this study, a recombinant anti-dengue D8 monoclonal antibody was produced in Nicotiana benthamiana and characterized for protein integrity and glycosylation by LC-MS. The…
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Exploring differential gene correlations in Arabidopsis thaliana for sustainable space farming
The plans for sustained human presence on the Moon have increased interest in understanding the effects of lunar conditions on terrestrial biology. Plants play a vital role in space exploration, as they not only generate oxygen and remove carbon dioxide but also provide fresh food that can be rich…
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Artikel Jurnal
Neural Latent Filtering for Gene Discovery in Breast Cancer Subtypes
Gene selection from expression data represents a challenging task, primarily due to the high data dimensionality and the vast number of genes that would be identified, many of which may be unrelated to cancer-relevant biological processes. To tackle this issue, filtering methods constitute an…
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Bacillus altitudinis GG-22: A novel plant growth-promoting bacterium with beneficial agronomic properties
Bacillus altitudinis GG-22, isolated from the phyllosphere of agricultural crops, has been identified as a promising biocontrol agent and plant growth-promoting bacterium with substantial potential in sustainable agriculture. In this study, whole-genome sequencing using Illumina technology…
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Valorization of agricultural residues for bioplastic production by bacteria isolated from plastic dumpsites: Integrating waste streams into the circular bioeconomy
Polyhydroxybutyrate (PHB) is a biodegradable polymer produced by bacteria under nutrient-limiting conditions, offering a sustainable alternative to petroleum plastics. This study investigated PHB production by bacterial isolates from plastic-contaminated soils. Forty isolates were screened using…
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Cyanobacterium-Penicillium co-flocculation under erythromycin treatment: increased CO2 capture and biomass production in an organic compound-free co-cultivation
Photoautotrophic cyanobacteria convert CO₂ into bioproducts. However, efficient cell harvesting remains a major obstacle. Auto-bioflocculation offers a promising solution. In cultures of Synechocystis sp. PCC 6803, Synechocystis co-flocculated with natural fungal contaminant (identified as…
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Production and characterization of rNGFSP: a recombinant fusion immunogen eliciting dual anti-NGF and anti-Substance P therapeutic antibodies for Degenerative Joint Disease
Anti-NGF monoclonal antibodies have recently been approved for treating degenerative joint disease, including osteoarthritis pain, in dogs and cats. However, their widespread use is limited by high cost and the requirement for repeated injections. Nerve Growth Factor and Substance P play central…
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Artikel Jurnal
Insulin Resistance, Anti-inflammatory, and Antioxidant In vitro Activity of Heterologously Expressed Arenin from Dryophytes arenicolor
Amphibian-derived peptides are promising therapeutics for metabolic dysfunction, oxidative stress, and immune imbalance. We investigated arenin, a Kunitz-type peptide from Dryophytes arenicolor, produced heterologously in E. coli, for anti-metabolic, antioxidant, and immunomodulatory activities. In…
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Plant-produced monoclonal antibodies recognize the native circumsporozoite protein on Plasmodium falciparum sporozoites
Malaria is primarily caused by Plasmodium parasites and remains a major global health threat due to drug resistance and limited vaccine efficacy. Monoclonal antibodies (mAbs) targeting the conserved NANP repeat region of the circumsporozoite protein (PfCSP), such as MS-1797, represent a promising…
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Artikel Jurnal
Pathogenesis and advances in immunotherapy for type 1 diabetes treatment
Type 1 diabetes (T1D) is an autoimmune disease marked by selective destruction of pancreatic β-cells, resulting in absolute insulin deficiency. Although insulin replacement remains the standard therapy, it does not address the underlying autoimmune process or prevent long-term complications…
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Artikel Jurnal
EIOFX-DT: Leveraging graph centrality metrics for feature extraction and classification of viral genetic sequences
Many diseases have a genetic origin, and analyzing intracellular structures through genetic data yields specific features for the diagnosis and classification of viral disease samples. In this study, 30 types of viruses were analyzed using a graph-based approach on genetic data. Genetic data has…
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Indole-alkaloid–rich fraction of Ervatamia coronaria leaf extract regresses breast cancer by inducing apoptotic cell death
Current anti-breast cancer therapies often cause severe toxicity to normal cells and promote chemoresistance, while their exorbitant costs limit their accessibility to many patients. This underscores the need for safer, effective, and affordable alternative treatment strategies. In this study, we…
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Extremophilic microbial isolates and metagenomic analysis of Greek and Hungarian bauxite residues
Bauxite residue (BR) is an extreme environment for microorganisms. The aim of the work was to isolate extremophilic microorganisms for further biotechnological applications, such as bioleaching or waste rehabilitation. At the same time, metagenomic analysis was performed to monitor short-term…
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Artikel Jurnal
Development of Saccharomyces cerevisiae isobutanol production strain from osmotolerant and ethanol-producing industrial isolated yeast
Isobutanol is a promising advanced biofuel alternative to ethanol for advanced biofuels and can be converted into sustainable aviation fuel (SAF) via the alcohol-to-jet (ATJ) process. Recent studies attempted to improve isobutanol production from S. cerevisiae by metabolic engineering…
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Overcoming challenges in the production of a human-like L-asparaginase: advanced strategies for expression and purification
Human l-asparaginase 1 (hASNase1) is a promising next-generation candidate for Acute Lymphoblastic Leukemia (ALL) therapy due to its reduced immunogenicity and superior physiological compatibility. However, its recombinant production remains challenging, as expression in Escherichia coli typically…
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From screening to sequencing: Investigating the monoethylene glycol (MEG) biodegradation by Stutzerimonas stutzeri EX72 strain to optimize Oil and Gas industrial produced water treatment
Monoethylene glycol (MEG) is widely used as a hydrate inhibitor in produced water operations; however, its presence complicates treatment processes by increasing chemical oxygen demand (COD) and posing potential environmental risks to receiving water bodies. This study aimed to isolate and identify…
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Artikel Jurnal
Comparative analysis of anti-MICA scFv affinities: Insights from three label-free biophysical methods and biological validation
Antibody–antigen affinity is a key factor in the development of therapeutic antibodies, influencing candidate selection and their clinical efficacy. We used a combination of three label-free biophysical techniques: SPR, ITC, and fluorescence quenching, to rank anti-MICA single-chain variable…