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QRB Discovery
Scopus
ISSN 2633-2892 · Cambridge University Press · 107 artikel di indeks
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Artikel Jurnal
Infrared nanoscopy for subcellular chemical imaging
Infrared (IR) nanoscopy represents a collection of imaging and spectroscopy techniques capable of resolving IR absorption on the nanometer scale. Chemical specificity is leveraged from vibrational spectroscopy, while light–matter interactions are detected by observing perturbations in the optical…
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Artikel Jurnal
HDX-MS reveals structurally dynamic mechanisms of drug action: Insights from deubiquitinase inhibitors
Protein conformational heterogeneity presents a major challenge for structure determination and structure-based inhibitor design, particularly for enzymes containing flexible or intrinsically disordered regions. X-ray crystallography and cryo-EM often capture only static conformations and…
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Artikel Jurnal
Uncovering the hidden biology of fibrinaloid microclot complexes in complex, inflammatory diseases
Blood can clot into anomalous, fibrinolysis-resistant forms that arise from prothrombotic seeding areas, including damaged cellular debris and membrane-derived surfaces, giving rise to what we have termed fibrinaloid microclot complexes (colloquially: microclots). Their proteolytic resistance is…
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Artikel Jurnal
Recent advances of in situ and in vivo electrochemical analysis of brain chemistry at micro- and nanoscale
Real-time, in situ monitoring of neurochemical dynamics in intact neural circuits is critical for elucidating brain function. Recent innovations in micro- and nanoelectrode engineering have markedly advanced our ability to detect neurotransmitter and neuromodulator release with high spatiotemporal…
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Artikel Jurnal
Mass spectrometry integrates protein design into structural biology method development
Recent advances in machine learning (ML) have transformed protein science, enabling engineering and de novo design of artificial proteins with novel structures and functions. However, experimental analysis of key design features, such as oligomerization, folding, ligand binding, and dynamic…
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Artikel Jurnal
Hole migration in cytochrome P450
The cytochrome P450 enzymes catalyze the hydroxylation of organic substrates by dioxygen. The high-potential reactive intermediate in cytochrome P450 catalysis, compound I (CI), has the capacity to deliver oxidizing equivalents (holes) to the side chains of tryptophan, tyrosine, and cysteine amino…
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Artikel Jurnal
Electrochemical measurements at human iPSC-derived FOXA2 dopaminergic neurons suggest a role for partial release in presynaptic plasticity
During the past decade, emerging studies using electrochemistry and nanoscale imaging have demonstrated that partial exocytotic release is prevailing in neuroendocrine cell models. However, due to complicated structure and culture process, few studies have been carried out using neurons, especially…
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Artikel Jurnal Teks lengkap
Structural bioinformatic study of human mitochondrial respiratory integral membrane megacomplex and its AlphaFold3 predicted water-soluble QTY megacomplex analog
Human mitochondrial Complex I is one of the largest multi-subunit membrane protein megacomplexes, which plays a critical role in oxidative phosphorylation and ATP production. It is also involved in many neurodegenerative diseases. However, studying its structure and the mechanisms underlying proton…
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Artikel Jurnal Teks lengkap
An integrated structural and biophysical approach to study carbon metabolism in Mycobacterium tuberculosis
Metabolic enzymes are the catalysts that drive the biochemical reactions essential for sustaining life. Many of these enzymes are tightly regulated by feedback mechanisms. To fully understand their roles and modulation, it is crucial to investigate the relationship between their structure…
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Artikel Jurnal
The conformational landscape of TRiC ring-opening and its underlying stepwise mechanism revealed by cryo-EM
The TRiC/CCT complex assists in the folding of approximately 10% of cytosolic proteins through an ATP-driven conformational cycle, playing a crucial role in maintaining protein homeostasis. Despite our understanding of ATP-driven TRiC ring closing and substrate folding, the process and mechanisms…
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Artikel Jurnal
A workflow to define structural classes and classify nucleic acids circular dichroism spectra
Circular dichroism (CD) spectroscopy is a widely utilized technique for studying the structures of chiral molecules, including nucleic acids. It is particularly valued for its ability to quickly probe structural changes in these biomolecules. Despite its potential, the prediction of nucleic acid…
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Artikel Jurnal
Thermodynamic control of gene regulation
G-quadruplexes and i-motifs are non-canonical secondary structures of DNA that act as conformational switches in controlling genomic events. Within the genome, G- and C-rich sequences with the potential to fold into G-quadruplexes and i-motifs are overrepresented in important regulatory domains…
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Artikel Jurnal
Unraveling the nature of physicochemical and biological processes underlying vesicular exocytotic release events through modeling of amperometric current spikes
This work offers a comprehensive approach to understanding the phenomena underlying vesicular exocytosis, a process involved in vital functions of living organisms such as neuronal and neuroendocrine signaling. The kinetics of release of most neuromediators that modulate these functions in various…
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Artikel Jurnal
On the possibility of carbon-free heteropolymers on Venus: a computational astrobiology study
This work poses and partially explores an astrobiological hypothesis: might polymeric sulfur and phosphorus-based oxides form heteropolymers in the acidic cloud decks of Venus’ atmosphere? Following an introduction to the emerging field of computational astrobiology, we demonstrate the use of…
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Artikel Jurnal
Continuous-time random walk model for the diffusive motion of helicases
DNA helicases are molecular motors that use the energy from nucleotide hydrolysis to move along DNA, promoting the unwinding or rewinding of the double helix. Here, we use magnetic and optical tweezers to track the motion of three helicases, gp41, RecQ, and RecG, while they unwind or rewind a DNA…
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Artikel Jurnal
Probing DNA melting behaviour under vibrational strong coupling
Manipulating matter by strong coupling to the vacuum field has attracted intensive interests over the last decade. In particular, vibrational strong coupling (VSC) has shown great potential for modifying ground state properties in solution chemistry and biochemical processes. In this work, the…
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Artikel Jurnal
Insights on the effect of macromolecular crowding on transcription and its regulation
Transcription of DNA into RNA is a fundamental cellular process upon which life depends. It is tightly regulated in several different ways, and among the most important mechanisms are protein-induced topological changes in DNA such as looping. In vivo neither transcription, nor protein-induced…
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Artikel Jurnal
Unleashing virus structural biology: Probing protein and membrane intermediates in the dynamic process of membrane fusion
Viruses are highly dynamic macromolecular assemblies. They undergo large-scale changes in structure and organization at nearly every stage of their infectious cycles from virion assembly to maturation, receptor docking, cell entry, uncoating and genome delivery. Understanding structural…