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BMC Molecular and Cell Biology
Scopus
ISSN 2661-8850 · BMC · 401 articles in the index
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Journal Article
Genetic suppressor of autophagy defects in huntingtin null cells identified using a mutagenesis screen in Dictyostelium discoideum
Abstract Background Huntingtin (HTT) is an important gene for cellular processes such as autophagy, and its loss leads to neurodegenerative disease phenotypes. In Dictyostelium discoideum, HTT-null (htt-) cells exhibit impaired basal autophagy and fail to develop in the presence of ammonium…
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Journal Article
Effects of irisin on inflammatory and apoptotic markers in the Caco-2 colon cancer cell line
Abstract Objective Irisin is a myokine that is secreted by muscle tissue and is recognized for its regulatory effects on inflammation. The objective of this study was to examine the potential anti-inflammatory function of irisin in the context of colon cancer, with a focus on its effects on the…
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Journal Article
Dysregulation of caspase-8 and caspase-9 in T-lymphocyte apoptosis: implications for pathogenesis, diagnosis, and therapeutic targeting in psoriatic arthritis
Abstract Background Apoptotic changes in keratinocytes and alterations in T-lymphocytes were shown to have a role in the progression of psoriatic arthritis (PsA). Objectives To determine the possible role of the apoptotic changes in peripheral T-lymphocytes in association with the activity of both…
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Journal Article
Mitochondrial HMGCS1 mediates cisplatin resistance in cervical cancer through regulation of mitochondrial transcription
Abstract Cisplatin resistance remains a major challenge in the treatment of cervical cancer. Here, we identify 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1), a rate-limiting enzyme in the mevalonate pathway, as a critical regulator of cisplatin resistance through its localization to…
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Journal Article
Intracellular Ca2+ is not essential for SHH signaling but is promoted by Shh ligand in embryonic fibroblasts
Abstract Background Sonic Hedgehog (SHH) signaling pathway controls cell proliferation, differentiation, and organ formation. The changes in Ca2+ and their regulatory role in the context of activated SHH signaling have not been fully examined. Results In this study, we induced activation of the SHH…
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Journal Article
RBM39 upregulation promotes ostium re-obstruction after endoscopic endonasal dacryocystorhinostomy (En-DCR)
Abstract Purpose Chronic dacryocystitis, characterized by epiphora, is primarily treated with dacryocystorhinostomy (DCR), yet postoperative anastomotic re-obstruction remains a significant cause of failure. This study aimed to identify the key molecular mechanisms driving this recurrence. Methods…
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Journal Article
Immunomodulatory effects of TAK-242 on osteogenesis of mesenchymal stem cells in acute inflammatory conditions
Abstract Background Bone regeneration involves a complex series of stages that need proper regulation of the acute inflammatory response. Toll-like receptor 4 (TLR4) is expressed in cells necessary for bone metabolism and modulates inflammatory conditions. However, excessive TLR4 activation can…
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Journal Article
A goat-derived gamma-tubulin antibody for triple-channel imaging of primary cilia
Abstract Background Primary cilia are essential sensory organelles, and their dysfunction leads to a broad spectrum of diseases known as ciliopathies. Immunofluorescence imaging of ciliary substructures is a key tool in cilia research, but multiplex staining is often limited by the availability of…
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Journal Article
Differential phosphorylation of PHIP phosphopeptides with implications in insulin signaling
Abstract Background PHIP (PH Interacting Protein) is an Insulin Receptor Substrate 1 (IRS1) interactor protein that binds to acetylated IRS1 through its bromodomain. Due to its involvement in insulin signaling pathway, it is implicated in various malignancies and metabolic disorders. Despite this…
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Journal Article
From microscope to model: rotating signaling dynamics and cluster size in Dictyostelium discoideum aggregates
Abstract Background When starved, Dictyostelium discoideum cells form clusters that, when constrained in height, exhibit clear collective rotational motion. During this collective rotation, cells secrete and relay the chemoattractant cAMP, resulting in signal propagation in the form of spiral…
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Journal Article
Hypoxia-pretreated exosomes promote enterocutaneous fistula healing via regulating inflammation and tissue repair
Abstract Background Enterocutaneous fistula (ECF) is an abnormal pathological passage. This study aims to investigate the role and mechanism of action of gelatin sponges loaded with hypoxic exosomes (GS-Hypo-Exos) in the treatment of enterocutaneous fistulas. Methods A rat ECF model was established…
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Journal Article
Chemoradiotherapy-induced Th17/IL-17 signaling correlates with therapeutic tolerance and early recurrence in cervical carcinoma
Abstract This study investigates the role of Th17 cells and IL-17 signaling in mediating therapeutic resistance and early recurrence in cervical carcinoma. Th17 cells were generated in vitro, and cervical carcinoma cells were subsequently exposed to cisplatin in the presence of Th17-conditioned…
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Journal Article
Telomere-to-telomere characterization of rDNA chromosome in the myxomycete Didymium iridis
Abstract Background The ribosomal DNA (rDNA) of the myxomycete Didymium iridis is located on a linear, multi-copy, non-Mendelian chromosome. Efforts to determine the complete sequence by short-read sequencing technologies have been prevented by the presence of highly repetitive regions. Here we use…
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Journal Article
Investigating the roles of macrophage age and circulating extracellular vesicles in cytoprotection of hypoxic renal tubular cell stress
Abstract Background Acute kidney injury (AKI) involves complex crosstalk between tubular epithelial cells (TECs) and immune cells. Extracellular vesicles (EVs) and ageing have emerged as modulators of this process. Methods We investigated the effects of circulating plasma EVs from young (Y_EVs) and…
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Journal Article
Insulin signaling engages divergent transcriptional mechanisms in neural and metabolic tissues
Abstract Background Insulin signaling is a conserved regulator of growth, metabolism, and lifespan across metazoans. While its systemic roles are well established, the mechanisms by which insulin coordinates tissue-specific transcriptional programs that underlie distinct functional demands remain…
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Journal Article
A nanobody-based degron system for targeted protein knockdown in Dictyostelium discoideum
Abstract Backgrounds The cellular slime mold Dictyostelium discoideum is a widely used model system for studying basic processes in cell and developmental biology. While genetic tools, such as targeted gene disruption by homologous recombination and genome editing using CRISPR/Cas9, are…
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Journal Article
Effects of prey quality through the life cycle of the social amoeba Dictyostelium discoideum
Abstract Background Generalist consumers can eat a variety of foods, but they are not necessarily equally beneficial. Different feeding histories can influence the fitness of genetically similar individuals. Dictyostelium discoideum is a unicellular generalist predator amoeba that eats bacteria…
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Journal Article
Human urine-derived stem exosomes inhibit inflammation and oxidative stress in sepsis-related acute kidney injury
Abstract Objective The objective of this study was to explore the mechanisms by which urine - derived stem cell exosomes (USCexo) alleviate sepsis - related acute kidney injury (SAKI), as acute kidney injury often complicates sepsis with unclear role of USCexo in SAKI while stem cell exosomes show…
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Journal Article
Dose-dependent effects of atorvastatin on inflammatory response in endothelial cells: MMP/TIMP balance and in vitro wound closure assay
Abstract Background Clinical and preclinical evidence suggests that inflammation is closely associated with various arterial diseases. Multiple studies have reported the effects of statins on different cell types, yet the effects of atorvastatin (ATV) and its mechanisms on inflamed HUVECs’ cellular…
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Journal Article
ALIX and ITCH localize to the base of primary cilia and negatively regulate ciliary Polycystin-2 levels
Abstract Primary cilia are antenna-like organelles that function as cellular hubs for signaling pathways, including Sonic hedgehog and signaling mediated by the Polycystin-1/Polycystin-2 cation channel complex. Proper regulation of signaling output depends on the dynamic control of ciliary protein…