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Academia Molecular Biology and Genomics
ISSN 3064-9765 · Academia.edu Journals · 80 articles in the index
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Journal Article
Characterization of two novel lytic bacteriophages for Klebsiella pneumoniae: molecular studies
Introduction: Multidrug-resistant Klebsiella pneumoniae (Kpn) poses a growing global health threat due to its role in severe hospital-acquired infections and increasing resistance to antibiotics. The objective of this study was to isolate, characterize, and evaluate the therapeutic potential of two…
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Journal Article
Exploring Streptomyces bacteriophage genomes for CRISPR elements
Introduction: Clustered regularly interspaced short palindromic repeats (CRISPR)-associated genes (CRISPR-Cas) is an acquired immune system that confers resistance in prokaryotes against invading extrachromosomal elements such as viruses and plasmids, and CRISPR editing technology is an important…
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Journal Article
Stress-induced iro gene expression in hypervirulent Klebsiella pneumoniae
Introduction: Klebsiella pneumoniae (K. pneumoniae) is a Gram-negative bacterium that poses a significant threat to public health. The virulence factors that enable its colonization and invasion of different anatomical sites, particularly iron acquisition, are critical, as iron limitation within…
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Journal Article
Molecular mechanisms and RNA biomarkers in cardiomyopathy diagnosis
Cardiomyopathy is a multifactorial cardiac disorder that results in structural and functional alterations in the heart due to molecular defects in the cardiomyocytes. It can manifest as hypertrophic, dilated, arrhythmogenic, or restrictive cardiomyopathies and affects around 1 in 500 persons…
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Journal Article
NGS-based MRD monitoring in mature B-cell and plasma cell malignancies
Minimal residual disease (MRD) is an important prognostic marker in mature B-cell and plasma cell malignancies, providing sensitive assessment of treatment response and risk of relapse. Next-generation sequencing (NGS) enables highly sensitive detection of MRD through identification and tracking of…
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Journal Article
From gene to therapy in MODY: β-cell mechanisms and precision treatment
Maturity-onset diabetes of the young (MODY) is a group of monogenic diabetes disorders marked by early-onset hyperglycemia and primary pancreatic β-cell dysfunction. MODY is often misclassified as type 1 or type 2 diabetes, which can lead to delayed genetic diagnosis and inappropriate therapy. This…
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Journal Article
Artificial intelligence and multi-omics integration in Alzheimer’s disease: translational challenges
The past two decades have witnessed a revolution in high-throughput omics technologies, which now offer unprecedented views of the molecular complexity underlying Alzheimer’s Disease (AD) and associated biomarkers. By leveraging nonlinear models and pattern recognition, artificial intelligence (AI)…
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Journal Article
Metatranscriptomic analyses of SARS-CoV-2 and humans reveal distinct differentially expressed genes
Introduction: Metatranscriptomic analyses provide critical insights into differentially expressed genes (DEGs) and their clinical implications. By leveraging wastewater-based epidemiology (WBE) and high-throughput sequencing, we can monitor viral pathogens cost-effectively, offering a robust…
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Journal Article
Messenger RNA alternative polyadenylation shapes plant development and virus interactions
As a co-transcriptional gene expression regulation process, pre-mRNA polyadenylation is a key co-transcriptional processing step for mRNA maturation. Polyadenylation affects the fate of mRNA by modulating its stability, translation efficiency, and nuclear-to-cytoplasmic translocation. Alternative…
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Journal Article
Revisiting qRT-PCR for gene expression profiling in single cells toward diagnostics
Quantitative reverse-transcription PCR (qRT-qPCR) remains one of the most precise methods for measuring transcript abundance, yet its application at single-cell resolution poses challenges that differ fundamentally from bulk workflows. As interest in single-cell biology grows, there is a pressing…
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Journal Article
Benchmarking multiple sequence alignment tools for SARS-CoV-2 genome and spike data
Introduction: One of the first steps in analyzing viral nucleotide/protein sequences is multiple sequence alignment (MSA). Due to the global effort of rapid diagnosis and advancement of sequencing technologies, more than three million SARS-CoV-2 genomes have been sequenced. This has given us an…
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Journal Article
Comparison of miRNAs and mRNA in bovine follicular fluid and granulosa cells
Introduction: Follicular condition is reflected in granulosa cells (GCs) and follicular fluid (FF), and miRNAs in GCs and FF play crucial roles in follicular and oocyte development.Materials and methods: In the present study, FF and GCs were collected from 10 cows, and miRNAs in FF and GCs, as well…
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Journal Article
RNA therapeutic approaches for dystrophic epidermolysis bullosa: a scoping review
Introduction: Dystrophic epidermolysis bullosa (DEB) is a severe genodermatosis caused by mutations in COL7A1, leading to deficient type VII collagen (C7). RNA-based therapeutics offer promising, mutation-specific alternatives. We aimed to map the preclinical and early translational research…
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Journal Article
Q0130 regulates mitochondrial energy metabolism and stress tolerance in yeast
Introduction: The growing demand for stress-resistant industrial microorganisms in green biomanufacturing has intensified research into the stress tolerance mechanisms of Saccharomyces cerevisiae. As the central organelle for energy metabolism and redox homeostasis, mitochondrial function is…
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Journal Article
Genetic and non-genetic sex determination in plants
Sexual reproduction arose at the dawn of the evolution of living beings and is a defining moment for Darwinian evolution and fitness. Its primary significance lies in the mixing of genes in a population and the creation of new genomic complexes in each new generation. From this perspective, the…
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Journal Article
Liquid–liquid phase separation as a driver of abiogenesis and evolution
The origin of life may have emerged through physical mechanisms that enable molecular organization and self-assembly. Building on Alexander Oparin’s concept of coacervate droplets, liquid–liquid phase separation (LLPS) offers a plausible route for prebiotic molecules to concentrate and interact…
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Journal Article
In silico analysis of TALE transcription factors in Rosa persica and investigation of their transcript levels in response to drought and cold stress
Introduction: The three-amino-acid-loop-extension (TALE) transcription factor family is widely present in plants and plays an important role in plant growth and development processes as well as responses to abiotic stresses. However, studies on the gene family are limited in Rosa persica. Materials…
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Journal Article
Epigenetic marks and promising epigenetic therapies
Epigenetic marks such as DNA methylation, histone modifications, and non-coding RNAs play a central role in regulating gene expression without altering the DNA sequence. These dynamic and reversible modifications contribute to cellular differentiation, genomic stability, and the fine-tuning of…
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Journal Article
Environmental impacts on skin genomics: molecular mechanisms of photoaging and protective strategies
Skin, as the body’s primary environmental interface, is continuously exposed to various environmental stressors that significantly impact its genomic integrity and accelerate aging processes. This mini review summarizes current knowledge of the molecular mechanisms behind environmentally induced…
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Journal Article
Harnessing the plant microbiome: a genomic and metagenomic perspective for sustainable agriculture
Plant-associated microbial communities play a pivotal role in agricultural productivity by enhancing crop yields, while reducing fertilizer dependency. Genome sequencing revolutionized the understanding of individual microbes, while metagenomics changed the game by analyzing genetic material…