8 hasil untuk "Corey Butler"
Menampilkan 1–8 · 0,88 detik · RSS
-
Artikel Jurnal
Multi-Dimensional Spectral Single Molecule Localization Microscopy
Single molecule localization (SML) and tracking (SPT) techniques, such as (spt)PALM, (u/DNA)PAINT and quantum dot tracking, have given unprecedented insight into the nanoscale molecular organization and dynamics in living cells. They allow monitoring individual proteins with millisecond temporal…
-
Artikel Jurnal
Bacterial cell wall nanoimaging by autoblinking microscopy
Abstract Spurious blinking fluorescent spots are often seen in bacteria during single-molecule localization microscopy experiments. Although this ‘autoblinking’ phenomenon is widespread, its origin remains unclear. In Deinococcus strains, we observed particularly strong autoblinking at the…
-
Artikel Jurnal
A tessellation-based colocalization analysis approach for single-molecule localization microscopy
Multicolour single-molecule localization microscopy lacks a standard analysis method. Here Levet et al. introduce Coloc-Tesseler, a parameter-free colocalisation analysis method based on tessellation analysis for the efficient analysis of multicolour SMLM data.
-
Artikel Jurnal
Mapping the dynamics and nanoscale organization of synaptic adhesion proteins using monomeric streptavidin
The advent of fluorescence-based super-resolution microscopy has created a need for labeling strategies relying on small probes that minimally perturb protein function. Here the authors describe a labeling method that reduces protein tag and label sizes, allowing for accurate protein targeting and…
-
Artikel Jurnal
Non-canonical interplay between glutamatergic NMDA and dopamine receptors shapes synaptogenesis
Abstract Direct interactions between receptors at the neuronal surface have long been proposed to tune signaling cascades and neuronal communication in health and disease. Yet, the lack of direct investigation methods to measure, in live neurons, the interaction between different membrane receptors…
-
Artikel Jurnal
In-depth single molecule localization microscopy using adaptive optics and single objective light-sheet microscopy
Abstract Single molecule localization microscopy (SMLM) allows deciphering the nanoscale organization and dynamics of biomolecules in their native environment with unprecedented resolution. While SMLM was quickly adopted by the scientific community for its performance and simple instrumentation, it…
-
Artikel Jurnal
NMDAR-dependent long-term depression is associated with increased short term plasticity through autophagy mediated loss of PSD-95
Long-term depression (LTD) of synaptic strength contributes to circuit remodeling, memory encoding and erasure. Here, the authors show that P2XR- and NMDAR-dependent LTD are associated with distinct and precise molecular modifications that lead to specific modification of synapse function.