7 hasil untuk "Mu-Rong Kao"
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Artikel Jurnal
Improvements of the productivity and saccharification efficiency of the cellulolytic β-glucosidase D2-BGL in Pichia pastoris via directed evolution
Abstract Background β-Glucosidases are essential for cellulose hydrolysis by catalyzing the final cellulolytic degradation of cello-oligomers and cellobiose to glucose. D2-BGL is a fungal glycoside hydrolase family 3 (GH3) β-glucosidase isolated from Chaetomella raphigera with high substrate…
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Artikel Jurnal
A unique self-truncation of bacterial GH5 endoglucanases leads to enhanced activity and thermostability
Abstract Background β-1,4-endoglucanase (EG) is one of the three types of cellulases used in cellulose saccharification during lignocellulosic biofuel/biomaterial production. GsCelA is an EG secreted by the thermophilic bacterium Geobacillus sp. 70PC53 isolated from rice straw compost in southern…
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Artikel Jurnal Teks lengkap
The Functional Characterization of an AA10 Lytic Polysaccharide Monooxygenase from Saccharophagus degradans 2-40T for Enhanced Chitin Biodegradation
Lytic polysaccharide monooxygenases (LPMOs) represent copper-dependent enzymes pivotal in breaking down resilient polysaccharides like cellulose and chitin by means of oxidation, creating more accessible sites for glycoside hydrolases. To elevate the conversion efficiency of chitin, an AA10 LPMO…
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Artikel Jurnal
From simple and specific zymographic detections to the annotation of a fungus Daldinia caldariorum D263 that encodes a wide range of highly bioactive cellulolytic enzymes
Abstract Background Lignocellulolytic enzymes are essential for agricultural waste disposal and production of renewable bioenergy. Many commercialized cellulase mixtures have been developed, mostly from saprophytic or endophytic fungal species. The cost of complete cellulose digestion is…
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Artikel Jurnal
The Gram-positive bacterium Romboutsia ilealis harbors a polysaccharide synthase that can produce (1,3;1,4)-β-d-glucans
Abstract (1,3;1,4)-β-d-Glucans are widely distributed in the cell walls of grasses (family Poaceae) and closely related families, as well as some other vascular plants. Additionally, they have been found in other organisms, including fungi, lichens, brown algae, charophycean green algae, and the…
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Artikel Jurnal
Chaetomella raphigera β-glucosidase D2-BGL has intriguing structural features and a high substrate affinity that renders it an efficient cellulase supplement for lignocellulosic biomass hydrolysis
Abstract Background To produce second-generation biofuels, enzymatic catalysis is required to convert cellulose from lignocellulosic biomass into fermentable sugars. β-Glucosidases finalize the process by hydrolyzing cellobiose into glucose, so the efficiency of cellulose hydrolysis largely depends…
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Artikel Jurnal
Poly-β-D-(1,6)-N-acetyl-glucosamine (PNAG) glycan vaccines with broad spectrum neutralizing activities
Abstract The development of bacterial vaccines is a complex challenge due to the substantial serological diversity of protective antigens. One promising antigenic target is the conserved surface polysaccharide poly-β-(1,6)-N-acetyl-D-glucosamine (PNAG). Despite its widespread distribution…