7 hasil untuk "Paul Datlinger"

Single-Cell DNA Methylome Sequencing and Bioinformatic Inference of Epigenomic Cell-State Dynamics

Matthias Farlik; Nathan C. Sheffield; Angelo Nuzzo dkk.

Cell Reports, Vol. 10 No. 8 · 2015 · Inggris · DOAJ Scopus

Methods for single-cell genome and transcriptome sequencing have contributed to our understanding of cellular heterogeneity, whereas methods for single-cell epigenomics are much less established. Here, we describe a whole-genome bisulfite sequencing (WGBS) assay that enables DNA methylation mapping…

Differential DNA Methylation Analysis without a Reference Genome

Johanna Klughammer; Paul Datlinger; Dieter Printz dkk.

Cell Reports, Vol. 13 No. 11 · 2015 · Inggris · DOAJ Scopus

Genome-wide DNA methylation mapping uncovers epigenetic changes associated with animal development, environmental adaptation, and species evolution. To address the lack of high-throughput methods for DNA methylation analysis in non-model organisms, we developed an integrated approach for studying…

Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing

Paul Datlinger; André F. Rendeiro; Thorina Boenke dkk.

Nature Methods · 2021 · Inggris · OpenAlex Scopus

Epigenome Mapping Reveals Distinct Modes of Gene Regulation and Widespread Enhancer Reprogramming by the Oncogenic Fusion Protein EWS-FLI1

Eleni M. Tomazou; Nathan C. Sheffield; Christian Schmidl dkk.

Cell Reports, Vol. 10 No. 7 · 2015 · Inggris · DOAJ Scopus

Transcription factor fusion proteins can transform cells by inducing global changes of the transcriptome, often creating a state of oncogene addiction. Here, we investigate the role of epigenetic mechanisms in this process, focusing on Ewing sarcoma cells that are dependent on the EWS-FLI1 fusion…

High-content CRISPR screening

Christoph Bock; Paul Datlinger; Florence M. Chardon dkk.

Nature Reviews Methods Primers · 2022 · Inggris · OpenAlex Scopus

Buffy coat signatures of breast cancer risk in a prospective cohort study

Felicia Fei-Lei Chung; Sandra González Maldonado; Amelie Nemc dkk.

Clinical Epigenetics, Vol. 15 No. 1 · 2023 · Inggris · DOAJ Scopus

Abstract Background Epigenetic alterations are a near-universal feature of human malignancy and have been detected in malignant cells as well as in easily accessible specimens such as blood and urine. These findings offer promising applications in cancer detection, subtyping, and treatment…

Comparative analysis of genome-scale, base-resolution DNA methylation profiles across 580 animal species

Johanna Klughammer; Daria Romanovskaia; Amelie Nemc dkk.

Nature Communications, Vol. 14 No. 1 · 2023 · Inggris · DOAJ Scopus

DNA methylation is involved in regulatory processes throughout the animal kingdom. Here, the authors map DNA methylation in 535 vertebrates and 45 invertebrates, establishing a reference dataset for cross-species analysis and exploring epigenetic variation across vertebrate evolution.