6 hasil untuk "Jason C. Lin"

Electroporation-Mediated Genome Editing of Livestock Zygotes

Jason C. Lin; Alison L. Van Eenennaam

Frontiers in Genetics, Vol. 12 · 2021 · Inggris · DOAJ Scopus

The introduction of genome editing reagents into mammalian zygotes has traditionally been accomplished by cytoplasmic or pronuclear microinjection. This time-consuming procedure requires expensive equipment and a high level of skill. Electroporation of zygotes offers a simplified and more…

The Need for Targeted Prevention and Screening in Gastrointestinal Cancer Disparities

Jason C. Lin; John C. Lin; Joy Zhao

Gastro Hep Advances, Vol. 4 No. 10 · 2025 · Inggris · DOAJ Scopus

Racial and Ethnic Differences in the Clinical Presentation of Celiac Disease in the United States: A Multi-Institutional Retrospective Analysis

Joy Zhao; John C. Lin; Jason C. Lin dkk.

Gastro Hep Advances, Vol. 5 No. 6 · 2026 · Inggris · DOAJ Scopus

A deletion at the polled PC locus alone is not sufficient to cause a polled phenotype in cattle

Sadie L. Hennig; Joseph R. Owen; Jason C. Lin dkk.

Scientific Reports, Vol. 12 No. 1 · 2022 · Inggris · DOAJ Scopus

Abstract Dehorning is a common practice in the dairy industry, but raises animal welfare concerns. A naturally occurring genetic mutation (PC allele) comprised of a 212 bp duplicated DNA sequence replacing a 10-bp sequence at the polled locus is associated with the hornless phenotype (polled) in…

Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos

Sadie L. Hennig; Joseph R. Owen; Jason C. Lin dkk.

Scientific Reports, Vol. 10 No. 1 · 2020 · Inggris · DOAJ Scopus

Abstract The CRISPR/Cas9 genome editing tool has the potential to improve the livestock breeding industry by allowing for the introduction of desirable traits. Although an efficient and targeted tool, the CRISPR/Cas9 system can have some drawbacks, including off-target mutations and mosaicism…

One-step generation of a targeted knock-in calf using the CRISPR-Cas9 system in bovine zygotes

Joseph R. Owen; Sadie L. Hennig; Bret R. McNabb dkk.

BMC Genomics, Vol. 22 No. 1 · 2021 · Inggris · DOAJ Scopus

Abstract Background The homologous recombination (HR) pathway is largely inactive in early embryos prior to the first cell division, making it difficult to achieve targeted gene knock-ins. The homology-mediated end joining (HMEJ)-based strategy has been shown to increase knock-in efficiency…