33 hasil untuk "P. Fuchs"
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Artikel Jurnal
Promise and problems associated with the use of recombinant AAV for the delivery of anti-HIV antibodies
Attempts to elicit antibodies with potent neutralizing activity against a broad range of human immunodeficiency virus (HIV) isolates have so far proven unsuccessful. Long-term delivery of monoclonal antibodies (mAbs) with such activity is a creative alternative that circumvents the need for an…
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Artikel Jurnal
Enrichment of plasmid pool from E. coli for mass sequencing to detect untargeted mutagenic events
Summary: Translesion synthesis (TLS) is an event to cope with DNA damages. During TLS, the responsible TLS polymerase frequently elicits untargeted mutagenesis as potentially a source of genetic diversity. Identifying such untargeted mutations in vivo is challenging due to the bulk of DNA that does…
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Artikel Jurnal Teks lengkap
Bacterial Proliferation: Keep Dividing and Don't Mind the Gap.
DNA Damage Tolerance (DDT) mechanisms help dealing with unrepaired DNA lesions that block replication and challenge genome integrity. Previous in vitro studies showed that the bacterial replicase is able to re-prime downstream of a DNA lesion, leaving behind a single-stranded DNA gap. The question…
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Artikel Jurnal Teks lengkap
Mass balance estimation on the Zongo glacier, Bolivia, using a semi-distributed conceptual model (SCM)
The mass balance of a glacier is used to quantify the accumulation and melting processes, which affect its mass. With this information, it is possible to infer the variation in water reserves, which will support decision-making based on the supply and demand of water. The objective of this work is…
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Artikel Jurnal Teks lengkap
Identification of Proteins Specifically Assembled on a Stem-Loop Composed of a CAG Triplet Repeat
Human genomic DNA contains a number of diverse repetitive sequence motifs, often identified as fragile sites leading to genetic instability. Among them, expansion events occurring at triplet repeats have been extensively studied due to their association with neurological disorders, including…
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Artikel Jurnal
Digital Power Network Parameters Measurement
Exact measurement of the parameters of a power network is nowpossible by digital methods. The description of the proposed andrealized instrument based on the digital sampling method is given. Itcan measure basic parameters of the three-phase power network such asrms values of voltages and currents…
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Artikel Jurnal Teks lengkap
Recombinant AAV Vectors for Enhanced Expression of Authentic IgG.
Adeno-associated virus (AAV) has become a vector of choice for the treatment of a variety of genetic diseases that require safe and long-term delivery of a missing protein. Muscle-directed gene transfer for delivery of protective antibodies against AIDS viruses and other pathogens has been used…
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Processing closely spaced lesions during Nucleotide Excision Repair triggers mutagenesis in E. coli.
It is generally assumed that most point mutations are fixed when damage containing template DNA undergoes replication, either right at the fork or behind the fork during gap filling. Here we provide genetic evidence for a pathway, dependent on Nucleotide Excision Repair, that induces mutations when…
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Glycoengineering of AAV-delivered monoclonal antibodies yields increased ADCC activity
The absence of fucose on asparagine-297 of the human immunoglobulin G (IgG) heavy chain has been shown to enhance antibody-dependent cellular cytotoxicity (ADCC) activity by 10- to 100-fold compared to fucosylated antibody. Our lab is studying the use of adeno-associated virus (AAV) as a vector for…
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Artikel Jurnal
Liver-Directed but Not Muscle-Directed AAV-Antibody Gene Transfer Limits Humoral Immune Responses in Rhesus Monkeys
A number of publications have described the use of adeno-associated virus (AAV) for the delivery of anti-HIV and anti-simian immunodeficiency virus (SIV) monoclonal antibodies (mAbs) to rhesus monkeys. Anti-drug antibodies (ADAs) have been frequently observed, and long-term AAV-mediated delivery…
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Artikel Jurnal
Effectiveness of virtual fencing in a mountain environment and its impact on heifer behaviour and welfare
Virtual fencing (VF) could be beneficial in mountain areas where electric wire fencing is difficult and time-consuming. However, environmental challenges of mountain pastures may impair VF efficacy and functionality, with potential effects on animal behaviour and welfare. Thirty female heifers were…
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Artikel Jurnal Teks lengkap
AAV-Delivered Antibody Mediates Significant Protective Effects against SIVmac239 Challenge in the Absence of Neutralizing Activity.
Long-term delivery of potent broadly-neutralizing antibodies is a promising approach for the prevention of HIV-1 infection. We used AAV vector intramuscularly to deliver anti-SIV monoclonal antibodies (mAbs) in IgG1 form to rhesus monkeys. Persisting levels of delivered mAb as high as 270 μg/ml…
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Indium-Free PTB7/PC71BM Polymer Solar Cells with Solution-Processed Al:ZnO Electrodes on PET Substrates
Inverted PTB7/PC71BM polymer solar cells are prepared on solution-processed Al:ZnO transparent contacts on PET substrates. Al:ZnO is deposited by a low temperature chemical bath deposition route (T < 100°C at any step) to comply with the temperature sensitive substrate. A maximum conversion…
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Artikel Jurnal
Cell Surface Display of a Single-Chain Antibody for Attaching Polypeptides
To provide an efficient means of coupling proteins, peptides and other suitable moieties to cells, we have constructed a retroviral expression vector for cell surface display of a single-chain antibody (scFv) against the hapten 4-ethoxymethylene-2-phenyl-oxazoline-5-one (phOx). The hapten phOx can…