14 hasil untuk "Robbie Loewith"

Regulation of Cellular Metabolism through Phase Separation of Enzymes

Manoël Prouteau; Robbie Loewith

Biomolecules, Vol. 8 No. 4 · 2018 · Inggris · DOAJ Scopus

Metabolism is the sum of the life-giving chemical processes that occur within a cell. Proper regulation of these processes is essential for all organisms to thrive and prosper. When external factors are too extreme, or if internal regulation is corrupted through genetic or epigenetic changes…

Chemical-Biology-derived in vivo Sensors: Past, Present, and Future

Robbie Loewith; Aurélien Roux; Olivier Pertz

CHIMIA, Vol. 75 No. 12 · 2021 · Prancis · DOAJ Scopus

To understand the complex biochemistry and biophysics of biological systems, one needs to be able to monitor local concentrations of molecules, physical properties of macromolecular assemblies and activation status of signaling pathways, in real time, within single cells, and at high…

Decoding cancer‐associated ribosomal protein mutations: A new frontier in cancer and ribosome biology

Mirsada Čaušević; Robbie Loewith; Siniša Volarević

HemaSphere, Vol. 10 No. 9 · 2026 · Inggris · DOAJ Scopus

Identification of a Covalent Importin‑5 Inhibitor, Goyazensolide, from a Collective Synthesis of Furanoheliangolides

Weilong Liu; Rémi Patouret; Sofia Barluenga dkk.

ACS Central Science, Vol. 7 No. 6 · 2021 · Inggris · DOAJ Scopus

Cryo-EM structure of Saccharomyces cerevisiae target of rapamycin complex 2

Manikandan Karuppasamy; Beata Kusmider; Taiana M. Oliveira dkk.

Nature Communications, Vol. 8 No. 1 · 2017 · Inggris · DOAJ Scopus

Target of rapamycin (TOR) kinase operates within two distinct multiprotein complexes named TORC1 and TORC2. Here the authors report a cryo-EM structure of TORC2, establish its subunit organization, providing a rationale for TORC2’s rapamycin insensitivity and the mutually exclusive inclusion of…

Dynamic metabolome profiling uncovers potential TOR signaling genes

Stella Reichling; Peter F Doubleday; Tomas Germade dkk.

eLife, Vol. 12 · 2023 · Inggris · DOAJ

Although the genetic code of the yeast Saccharomyces cerevisiae was sequenced 25 years ago, the characterization of the roles of genes within it is far from complete. The lack of a complete mapping of functions to genes hampers systematic understanding of the biology of the cell. The advent of…

A dynamic feedback loop between retrograde sterol transport and TORC2 controls adaptation of the plasma membrane to stress

Maria G Tettamanti; Paulina Nowak; Beata Kusmider dkk.

The EMBO Journal, Vol. 44 No. 24 · 2025 · Inggris · DOAJ Scopus

Abstract Cells monitor and dynamically regulate the lipid composition and biophysical properties of their plasma membrane (PM). The Target Of Rapamycin complex 2 (TORC2) is a protein kinase that acts as a central regulator of plasma membrane homeostasis, but the mechanisms by which it detects and…

Active-Site Inhibitors of mTOR Target Rapamycin-Resistant Outputs of mTORC1 and mTORC2

Morris E Feldman; Beth Apsel; Aino Uotila dkk.

PLoS Biology, Vol. 7 No. 2 · 2009 · Inggris · PLOS Scopus

The mammalian target of rapamycin (mTOR) regulates cell growth and survival by integrating nutrient and hormonal signals. These signaling functions are distributed between at least two distinct mTOR protein complexes: mTORC1 and mTORC2. mTORC1 is sensitive to the selective inhibitor rapamycin and…

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Limited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells

Franziska Elsässer; Roberta Florea; Felix Räsch dkk.

Molecular Systems Biology, Vol. 22 No. 3 · 2026 · Inggris · DOAJ Scopus

Abstract The function of a protein is determined by its structure, which may change dynamically in response to post-translational modifications, interaction with other molecules, or environmental factors like temperature. Limited proteolysis-coupled mass spectrometry (LiP-MS) captures such…

A Neurotoxic Glycerophosphocholine Impacts PtdIns-4, 5-Bisphosphate and TORC2 Signaling by Altering Ceramide Biosynthesis in Yeast

Michael A. Kennedy; Kenneth Gable; Karolina Niewola-Staszkowska dkk.

PLoS Genetics, Vol. 10 No. 1 · 2014 · Inggris · PLOS Scopus

Unbiased lipidomic approaches have identified impairments in glycerophosphocholine second messenger metabolism in patients with Alzheimer's disease. Specifically, we have shown that amyloid-β42 signals the intraneuronal accumulation of PC(O-16:0/2:0) which is associated with neurotoxicity. Similar…

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Chemical Biology Approaches to Membrane Homeostasis and Function

Miwa Takahashi-Umebayashi; Kai Johnsson; Luc Reymond dkk.

CHIMIA, Vol. 65 No. 11 · 2011 · Prancis · DOAJ Scopus

The study of membranes is at a turning point. New theories about membrane structure and function have recently been proposed, however, new technologies, combining chemical, physical, and biochemical approaches are necessary to test these hypotheses. In particular, the NCCR in chemical biology aims…

Flipper Probes for the Community

Lea Assies; José García-Calvo; Francesca Piazzolla dkk.

CHIMIA, Vol. 75 No. 12 · 2021 · Prancis · DOAJ Scopus

This article describes four fluorescent membrane tension probes that have been designed, synthesized, evaluated, commercialized and applied to current biology challenges in the context of the NCCR Chemical Biology. Their names are Flipper-TR©, ER Flipper-TR©, Lyso Flipper-TR© and Mito Flipper-TR©…