6 hasil untuk "Valentin Dunsing"
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Artikel Jurnal
Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection
Signaling pathways in biological systems rely on specific interactions between multiple biomolecules. Fluorescence fluctuation spectroscopy provides a powerful toolbox to quantify such interactions directly in living cells. Cross-correlation analysis of spectrally separated fluctuations provides…
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Artikel Jurnal Teks lengkap
Benchmarking of novel green fluorescent proteins for the quantification of protein oligomerization in living cells
Protein-protein-interactions play an important role in many cellular functions. Quantitative non-invasive techniques are applied in living cells to evaluate such interactions, thereby providing a broader understanding of complex biological processes. Fluorescence fluctuation spectroscopy describes…
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Artikel Jurnal
Spatial patterning of contractility by a self-organized mechanogen activity gradient underlies Drosophila gastrulation
Abstract During development cell deformations are spatially organized, however, how cellular mechanics is spatially controlled is unclear. Spatial control of cell identity often determines local cellular mechanics in a two-tiered mechanism. Theoretical studies also proposed that molecular…
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Artikel Jurnal
A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales
Whole-mount 3D imaging at the cellular scale is a powerful tool for exploring complex processes during morphogenesis. In organoids, it allows examining tissue architecture, cell types, and morphology simultaneously in 3D models. However, cell packing in multilayered organoid tissues hinders both…
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Artikel Jurnal
Serotonin signaling regulates actomyosin contractility during morphogenesis in evolutionarily divergent lineages
Abstract Serotonin is a neurotransmitter that signals through 5-HT receptors to control key functions in the nervous system. Serotonin receptors are also ubiquitously expressed in various organs and have been detected in embryos of different organisms. Potential morphogenetic functions of serotonin…
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Artikel Jurnal
Fast volumetric fluorescence lifetime imaging of multicellular systems using single-objective light-sheet microscopy
Abstract Fluorescence lifetime imaging (FLIM) is widely used for functional and multiplexed bioimaging. The lifetime of autofluorescence or fluorescent sensors encodes physiologically relevant parameters. Thus, FLIM is especially relevant for the investigation of living systems. However…