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Artificial intelligence for research capacity strengthening: Two reviews and a pathway to shift power in global health
Significant disparities persist in how researchers from low- and middle-income countries (LMICs) and high-income countries (HICs) participate in agenda-setting and knowledge production. Rapid advancement in artificial intelligence (AI) might contribute to improving research capacity in LMICs. This…
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Correction: Pilot study assessing gut microbial diversity among sexual and gender minority young adults
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An empirical network study of the antimalarial supply chain in Ghana
A survey of a sample of Ghanaian pharmacies was undertaken to trace the antimalarial drug supply chain. We sampled antimalarial drug outlets in 6 districts across Ghana and traced their immediate suppliers. A third level of the supply chain was obtained by visiting these intermediate suppliers and…
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Mukara: A deep learning alternative to the four-step travel demand model with a case study on interurban highway traffic prediction in the UK
Accurate traffic volume prediction is essential for managing congestion, improving road safety, mitigating environmental impacts, and supporting long-term transportation planning. The traditional four-step travel demand model (FSM) is a well-established framework, but it relies on static survey…
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Co-design of Lifestyle6, a digital tool targeting multiple health behaviour changes for cancer risk reduction and early detection support
Up to 40% of all cancers diagnosed could be prevented through the uptake of healthy lifestyle behaviours. This paper describes the co-design of a novel community-centric digital programme for cancer prevention and early detection support. Twenty-two community panel members partook in a series…
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Effects of harvest number on the yield and quality of different alfalfa varieties under hydroponic conditions
Five alfalfa varieties, namely Zhongcao No.13, WL440HQ, WL525HQ, WL903, and WL712, were subjected to a hydroponic cultivation experiment under controlled conditions of 25℃ room temperature, a nutrient solution concentration of 1.5 times the standard Hoagland formulation, and a 10-hour photoperiod…
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Sanitation access and satisfaction in northern Haiti: Insights from a quasi-census survey
Between 2015 and 2024, global open defecation declined, yet fragile contexts continue to lag behind this trend. In Haiti, urban access to safe sanitation fell from 34% to 32%, with dense housing and frequent flooding limiting the potential of conventional solutions like sewers, septic tanks, and…
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The Physicochemical Synergy Effect of Nanosecond Pulsed Electric Fields (nsPEF) and cisplatin on reversing chemoresistance of bladder cancer
Nanosecond Pulsed Electric Field (nsPEF) is an intense electrical pulse technology with ultra-short duration (nanoseconds), capable of generating electric field intensities up to several kV/cm. By inducing irreversible electroporation for tumor tissue ablation, nsPEF triggers apoptosis in cancer…
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upsML: A high-accuracy machine learning classifier for predicting Plasmodium falciparum var gene upstream groups
Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), encoded by the hypervariable var gene family, is central to malaria pathogenesis, influencing both disease severity and immune evasion. Classifying var genes into upstream groups (upsA, upsB, upsC, upsE) is important for understanding…
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Methylviologen resistance in loss-of-function mutants of the polyamine transporter gene OsLAT5
TALENs and CRISPR/Cas have become routine tools for genome editing. During stable plant transformation, genes coding for editing enzymes, e.g., Cas9, guide RNAs (gRNA), and selectable or screenable markers are integrated into the nuclear genome. Identification of successful transformants relies on…
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The physical and psychological well-being after a pulmonary embolism across age and comorbidities – Evidence from focus group interviews
Background: Few studies have investigated the psychological and physical consequences of having experienced a pulmonary embolism (PE), and most patients with PE are not offered proper follow-up. Objective: To gain qualitative insight into the physical and psychological well-being across age and…
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Multi-adaptive event-triggered consensus of positive multi-agent systems using pinning strategy
This paper investigates the multi-adaptive event-triggered consensus of positive multi-agent systems. First, two classes of event-triggered mechanisms are designed for leaders and followers, respectively. A multi-adaptive event-triggered pinning control protocol is proposed by virtue of the…
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Systematic prioritization of potential therapeutic targets for glomerulonephritis using multi-omics Mendelian randomization
Glomerulonephritis (GN) is an immune-mediated kidney disorder that causes glomerular injury, progressive renal dysfunction, and end-stage kidney disease. Traditional treatments such as corticosteroids and immunosuppressants are limited by variable efficacy and severe adverse effects, highlighting…
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Susceptibility of wild and domestic songbirds to Usutu virus
Usutu virus (USUV) is an emerging mosquito-borne orthoflavivirus that can cause neuroinvasive disease in humans and wild birds. USUV clusters phylogenetically within the Japanese encephalitis virus serocomplex, sharing antigenic and ecological similarity with West Nile virus (WNV). USUV is…
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Burden of antimicrobial resistance in culture-confirmed Salmonella Typhi isolates in India from 1977 to 2024: A systematic review and meta-analysis
Background: Antimicrobial-resistant (AMR) Salmonella Typhi (S. Typhi) is a persistent public health threat in India. We conducted a systematic review and meta-analysis to estimate the prevalence and temporal trends of AMR S. Typhi across Indian states to inform prevention and treatment strategies…
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Cost-effectiveness of esketamine versus alternative treatment strategies for treatment-resistant depression in Hong Kong: A multi-armed modeling study
Background: Treatment-resistant depression (TRD), defined as failure to respond to at least two adequately administered antidepressant (AD) regimens, imposes major clinical and economic burdens. Esketamine nasal spray offers rapid antidepressant clinical effects, yet previous evaluations compared…
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An international consensus on core reproducibility items in research
Evidence-based solutions are needed to help improve reproducibility in research. This Consensus View presents a consensus-based list of core reproducibility items for research that has been developed by a multidisciplinary group interested in research, open science, and reproducibility. The set of…
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PLoS Biology Issue Image | Vol. 24(3) April 2026
Comparative embryogenesis of two salp species reveals rogue development and evolutionary divergence from sessile tunicates: Tunicates are the closest living relatives of vertebrates. Recent phylogenies place the little-studied, free-swimming thaliaceans—including salps—within sessile ascidians…
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Somatosensory input drives membrane potential dynamics in motor cortex during voluntary limb movement
How the motor cortex controls movement remains a fundamental question in neuroscience. Although somatosensory input is thought to influence motor cortex activity and the execution of voluntary movements, its role in driving motor cortex activity during voluntary behavior remains unclear. To address…
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