33 results for "Li-Lin Du"

Fission Yeast Autophagy Machinery

Dan-Dan Xu; Li-Lin Du

Cells, Vol. 11 No. 7 · 2022 · English · DOAJ Scopus

Autophagy is a conserved process that delivers cytoplasmic components to the vacuole/lysosome. It plays important roles in maintaining cellular homeostasis and conferring stress resistance. In the fission yeast Schizosaccharomyces pombe, autophagy is important for cell survival under nutrient…

Mdb1, a Fission Yeast Homolog of Human MDC1, Modulates DNA Damage Response and Mitotic Spindle Function

Yi Wei; Hai-Tao Wang; Yonggong Zhai et al.

PLoS ONE, Vol. 9 No. 5 · 2014 · English · PLOS Scopus

During eukaryotic DNA damage response (DDR), one of the earliest events is the phosphorylation of the C-terminal SQ motif of histone H2AX (H2A in yeasts). In human cells, phosphorylated H2AX (γH2AX) is recognized by MDC1, which serves as a binding platform for the accumulation of a myriad of DDR…

Also available at: DOAJ

Cryo‐EM structure of fission yeast tetrameric α‐mannosidase Ams1

Jianxiu Zhang; Ying‐Ying Wang; Li‐Lin Du et al.

FEBS Open Bio, Vol. 10 No. 11 · 2020 · English · DOAJ Scopus

Fungal α‐mannosidase Ams1 and its mammalian homolog MAN2C1 hydrolyze terminal α‐linked mannoses in free oligosaccharides released from misfolded glycoproteins or lipid‐linked oligosaccharide donors. Ams1 is transported by selective autophagy into vacuoles. Here, we determine the tetrameric…

Tdp1 processes chromate-induced single-strand DNA breaks that collapse replication forks

Abantika Ganguly; Lan Guo; Lingling Sun et al.

PLOS Genetics, Vol. 14 No. 8 · 2018 · English · PLOS Scopus

Hexavalent chromium [Cr(VI)] damages DNA and causes cancer, but it is unclear which DNA damage responses (DDRs) most critically protect cells from chromate toxicity. Here, genome-wide quantitative functional profiling, DDR measurements and genetic interaction assays in Schizosaccharomyces pombe…

Also available at: DOAJ

A Cloning-Free Method for CRISPR/Cas9-Mediated Genome Editing in Fission Yeast

Xiao-Ran Zhang; Jia-Bei He; Yi-Zheng Wang et al.

G3: Genes, Genomes, Genetics, Vol. 8 No. 6 · 2018 · English · DOAJ Scopus

The CRISPR/Cas9 system, which relies on RNA‐guided DNA cleavage to induce site-specific DNA double-strand breaks, is a powerful tool for genome editing. This system has been successfully adapted for the fission yeast Schizosaccharomyces pombe by expressing Cas9 and the single-guide RNA (sgRNA) from…

Global Fitness Profiling Identifies Arsenic and Cadmium Tolerance Mechanisms in Fission Yeast

Lan Guo; Abantika Ganguly; Lingling Sun et al.

G3: Genes, Genomes, Genetics, Vol. 6 No. 10 · 2016 · English · DOAJ Scopus

Heavy metals and metalloids such as cadmium [Cd(II)] and arsenic [As(III)] are widespread environmental toxicants responsible for multiple adverse health effects in humans. However, the molecular mechanisms underlying metal-induced cytotoxicity and carcinogenesis, as well as the detoxification and…

Phosphorylation-Dependent Interactions between Crb2 and Chk1 Are Essential for DNA Damage Checkpoint

Meng Qu; Bing Yang; Li Tao et al.

PLoS Genetics, Vol. 8 No. 7 · 2012 · English · PLOS Scopus

In response to DNA damage, the eukaryotic genome surveillance system activates a checkpoint kinase cascade. In the fission yeast Schizosaccharomyces pombe, checkpoint protein Crb2 is essential for DNA damage-induced activation of downstream effector kinase Chk1. The mechanism by which Crb2 mediates…

Also available at: DOAJ

A large gene family in fission yeast encodes spore killers that subvert Mendel’s law

Wen Hu; Zhao-Di Jiang; Fang Suo et al.

eLife, Vol. 6 · 2017 · English · DOAJ

Spore killers in fungi are selfish genetic elements that distort Mendelian segregation in their favor. It remains unclear how many species harbor them and how diverse their mechanisms are. Here, we discover two spore killers from a natural isolate of the fission yeast Schizosaccharomyces pombe…

An improved auxin-inducible degron system for fission yeast

Xiao-Ran Zhang; Lei Zhao; Fang Suo et al.

G3: Genes, Genomes, Genetics, Vol. 12 No. 1 · 2021 · English · DOAJ Scopus

AbstractConditional degron technologies, which allow a protein of interest to be degraded in an inducible manner, are important tools for biological research, and are especially useful for creating conditional loss-of-function mutants of essential genes. The auxin-inducible degron (AID) technology…

Atg1 kinase in fission yeast is activated by Atg11-mediated dimerization and cis-autophosphorylation

Zhao-Qian Pan; Guang-Can Shao; Xiao-Man Liu et al.

eLife, Vol. 9 · 2020 · English · DOAJ

Autophagy is a proteolytic pathway that is conserved from yeasts to mammals. Atg1 kinase is essential for autophagy, but how its activity is controlled remains insufficiently understood. Here, we show that, in the fission yeast Schizosaccharomyces pombe, Atg1 kinase activity requires Atg11, the…

Structural mechanism of protein recognition by the FW domain of autophagy receptor Nbr1

Jianxiu Zhang; Ying-Ying Wang; Zhao-Qian Pan et al.

Nature Communications, Vol. 13 No. 1 · 2022 · English · DOAJ Scopus

Nbr1 recognizes cargos in selective autophagy. Here, authors show filamentous yeast Nbr1 binds Ams1 via an FW domain, and the cryo-EM structure reveals that Nbr1 recognizes the N-terminal di-glycine and tetrameric assembly of Ams1.

CRL4 Cdt2 ubiquitin ligase regulates Dna2 and Rad16 (XPF) nucleases by targeting Pxd1 for degradation

Jia-Min Zhang; Jin-Xin Zheng; Yue-He Ding et al.

PLOS Genetics, Vol. 16 No. 7 · 2020 · English · PLOS Scopus

Structure-specific endonucleases (SSEs) play key roles in DNA replication, recombination, and repair. SSEs must be tightly regulated to ensure genome stability but their regulatory mechanisms remain incompletely understood. Here, we show that in the fission yeast Schizosaccharomyces pombe, the…

Also available at: DOAJ

Global Analysis of Fission Yeast Mating Genes Reveals New Autophagy Factors

Ling-Ling Sun; Ming Li; Fang Suo et al.

PLoS Genetics, Vol. 9 No. 8 · 2013 · English · PLOS Scopus

Macroautophagy (autophagy) is crucial for cell survival during starvation and plays important roles in animal development and human diseases. Molecular understanding of autophagy has mainly come from the budding yeast Saccharomyces cerevisiae, and it remains unclear to what extent the mechanisms…

The ortholog of human REEP1-4 is required for autophagosomal enclosure of ER-phagy/nucleophagy cargos in fission yeast

Chen-Xi Zou; Zhu-Hui Ma; Zhao-Di Jiang et al.

PLOS Biology, Vol. 21 No. 11 · 2023 · English · PLOS Scopus

Selective macroautophagy of the endoplasmic reticulum (ER) and the nucleus, known as ER-phagy and nucleophagy, respectively, are processes whose mechanisms remain inadequately understood. Through an imaging-based screen, we find that in the fission yeast Schizosaccharomyces pombe, Yep1 (also known…

A high-quality reference genome for the fission yeast Schizosaccharomyces osmophilus

Guo-Song Jia; Wen-Cai Zhang; Yue Liang et al.

G3: Genes, Genomes, Genetics, Vol. 13 No. 4 · 2023 · English · DOAJ Scopus

AbstractFission yeasts are an ancient group of fungal species that diverged from each other from tens to hundreds of million years ago. Among them is the preeminent model organism Schizosaccharomyces pombeS. pombeS. japonicusS. octosporusS. cryophilusS. osmophilusS. osmophilusS. octosporusS…

Mck1 defines a key S-phase checkpoint effector in response to various degrees of replication threats

Xiaoli Li; Xuejiao Jin; Sushma Sharma et al.

PLOS Genetics, Vol. 15 No. 8 · 2019 · English · PLOS Scopus

The S-phase checkpoint plays an essential role in regulation of the ribonucleotide reductase (RNR) activity to maintain the dNTP pools. How eukaryotic cells respond appropriately to different levels of replication threats remains elusive. Here, we have identified that a conserved GSK-3 kinase Mck1…