41 hasil untuk "Erik J Soderblom"

CDK12 Activity-Dependent Phosphorylation Events in Human Cells

Bartlomiej Bartkowiak; Christopher M. Yan; Erik J. Soderblom dkk.

Biomolecules, Vol. 9 No. 10 · 2019 · Inggris · DOAJ Scopus

We asked whether the C-terminal repeat domain (CTD) kinase, CDK12/CyclinK, phosphorylates substrates in addition to the CTD of RPB1, using our CDK12analog-sensitive HeLa cell line to investigate CDK12 activity-dependent phosphorylation events in human cells. Characterizing the phospho-proteome…

Hypothesis Testing With Proteomics: A Case Study Using Wound Healing Mechanisms in Fluids Associated With Barnacle Glue

Tara Essock-Burns; Erik J. Soderblom; Beatriz Orihuela dkk.

Frontiers in Marine Science, Vol. 6 · 2019 · Inggris · DOAJ Scopus

Growth, molting, and hardening cuticle are intertwined processes for arthropods and share common protein systems to execute these functions. For barnacles, these processes are also tied to adhesion, which is vital to their survival and under great selection pressure. Unlike other crustaceans…

Identification of Proteins Bound to Dengue Viral RNA In Vivo Reveals New Host Proteins Important for Virus Replication

Stacia L. Phillips; Erik J. Soderblom; Shelton S. Bradrick dkk.

mBio, Vol. 7 No. 1 · 2016 · Inggris · DOAJ Scopus

ABSTRACT Dengue virus is the most prevalent cause of arthropod-borne infection worldwide. Due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. Host RNA-binding proteins…

Dataset on the mass spectrometry-based proteomic profiling of mouse embryonic fibroblasts from a wild type and DYT-TOR1A mouse model of dystonia, basally and during stress

Kunal Shroff; Zachary F. Caffall; Erik J. Soderblom dkk.

Data in Brief, Vol. 39 · 2021 · Inggris · DOAJ Scopus

Here, we present quantitative subcellular compartment-specific proteomic data from wildtype and DYT-TOR1A heterozygous mouse embryonic fibroblasts (MEFs) basally and following thapsigargin (Tg) treatment [1]. In this experiment, we generated MEFs from wild type (WT) and a heterozygous DYT-TOR1A…

In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation

Erin F. Spence; Shataakshi Dube; Akiyoshi Uezu dkk.

Nature Communications, Vol. 10 No. 1 · 2019 · Inggris · DOAJ Scopus

The internal molecular mechanisms that underlie excitatory synaptogenesis remain poorly characterized. This study utilizes a chemogenetic approach, in vivo biotin identification (iBioID), to discover previously uncharacterized proteins at nascent synapses. CARMIL3 is identified as a cytoskeletal…

Peptide array X-linking (PAX): a new peptide-protein identification approach.

Hirokazu Okada; Akiyoshi Uezu; Erik J Soderblom dkk.

PLoS ONE, Vol. 7 No. 5 · 2012 · Inggris · DOAJ Scopus

Many protein interaction domains bind short peptides based on canonical sequence consensus motifs. Here we report the development of a peptide array-based proteomics tool to identify proteins directly interacting with ligand peptides from cell lysates. Array-formatted bait peptides containing an…

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A Novel Glycoproteomics Workflow Reveals Dynamic O-GlcNAcylation of COPγ1 as a Candidate Regulator of Protein Trafficking

Nathan J. Cox; Peter M. Luo; Timothy J. Smith dkk.

Frontiers in Endocrinology, Vol. 9 · 2018 · Inggris · DOAJ Scopus

O-linked β-N-acetylglucosamine (O-GlcNAc) is an abundant and essential intracellular form of protein glycosylation in animals and plants. In humans, dysregulation of O-GlcNAcylation occurs in a wide range of diseases, including cancer, diabetes, and neurodegeneration. Since its discovery more than…

Site-specific glycosylation of Sec24D and myoferlin recruit ERGIC to ER exit sites for collagen trafficking

Tetsuya Hirata; Dharmendra Choudhary; Quyen Nguyen dkk.

Nature Communications, Vol. 17 No. 1 · 2026 · Inggris · DOAJ Scopus

Abstract Coat protein complex II (COPII) mediates anterograde trafficking from the endoplasmic reticulum (ER). While the core COPII machinery is well-characterized, how cells regulate COPII to accommodate large cargoes, including collagens, remains incompletely understood. Here, we show that the…

Action potential-coupled Rho GTPase signaling drives presynaptic plasticity

Shataakshi Dube O'Neil; Bence Rácz; Walter Evan Brown dkk.

eLife, Vol. 10 · 2021 · Inggris · DOAJ

In contrast to their postsynaptic counterparts, the contributions of activity-dependent cytoskeletal signaling to presynaptic plasticity remain controversial and poorly understood. To identify and evaluate these signaling pathways, we conducted a proteomic analysis of the presynaptic cytomatrix…

Interaction between Rsp5-dependent ubiquitination and trehalose production during Cryptococcus neoformans temperature stress adaptation

Alejandro L. Antonia; Lukas M. du Plooy; Siobhan R. Duffy dkk.

mSphere, Vol. 11 No. 6 · 2026 · Inggris · DOAJ Scopus

ABSTRACT Microorganisms, including fungi, adapt to profound changes in their local environment during human infections. After exposure to high temperature and other stress conditions, the opportunistic fungal pathogen Cryptococcus neoformans enacts changes in metabolism, cell wall structure, and…

Calcineurin Targets Involved in Stress Survival and Fungal Virulence

Hee-Soo Park; Eve W. L. Chow; Ci Fu dkk.

PLOS Pathogens, Vol. 12 No. 9 · 2016 · Inggris · PLOS Scopus

Calcineurin governs stress survival, sexual differentiation, and virulence of the human fungal pathogen Cryptococcus neoformans. Calcineurin is activated by increased Ca2+ levels caused by stress, and transduces signals by dephosphorylating protein substrates. Herein, we identified and…

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Metabolic Catastrophe in Mice Lacking Transferrin Receptor in Muscle

Tomasa Barrientos; Indira Laothamatas; Timothy R. Koves dkk.

EBioMedicine, Vol. 2 No. 11 · 2015 · Inggris · DOAJ Scopus

Transferrin receptor (Tfr1) is ubiquitously expressed, but its roles in non-hematopoietic cells are incompletely understood. We used a tissue-specific conditional knockout strategy to ask whether skeletal muscle required Tfr1 for iron uptake. We found that iron assimilation via Tfr1 was critical…

Assessing Trans-Inhibition of OATP1B1 and OATP1B3 by Calcineurin and/or PPIase Inhibitors and Global Identification of OATP1B1/3-Associated Proteins

John T. Powell; Ruhul Kayesh; Alexandra Ballesteros-Perez dkk.

Pharmaceutics, Vol. 16 No. 1 · 2023 · Inggris · DOAJ Scopus

Organic anion transporting polypeptide (OATP) 1B1 and OATP1B3 are key determinants of drug–drug interactions (DDIs). Various drugs including the calcineurin inhibitor (CNI) cyclosporine A (CsA) exert preincubation-induced trans-inhibitory effects upon OATP1B1 and/or OATP1B3 (abbreviated as…

Photo-activated psoralen binds the ErbB2 catalytic kinase domain, blocking ErbB2 signaling and triggering tumor cell apoptosis.

Wenle Xia; David Gooden; Leihua Liu dkk.

PLoS ONE, Vol. 9 No. 2 · 2014 · Inggris · DOAJ Scopus

Photo-activation of psoralen with UVA irradiation, referred to as PUVA, is used in the treatment of proliferative skin disorders. The anti-proliferative effects of PUVA have been largely attributed to psoralen intercalation of DNA, which upon UV treatment, triggers the formation of interstrand DNA…

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Chemico-genetic discovery of astrocytic control of inhibition in vivo

Tetsuya Takano; John T. Wallace; Katherine T. Baldwin dkk.

Nature · 2020 · Inggris · OpenAlex Scopus

Genetic disruption of WASHC4 drives endo-lysosomal dysfunction and cognitive-movement impairments in mice and humans

Jamie L Courtland; Tyler WA Bradshaw; Greg Waitt dkk.

eLife, Vol. 10 · 2021 · Inggris · DOAJ

Mutation of the Wiskott–Aldrich syndrome protein and SCAR homology (WASH) complex subunit, SWIP, is implicated in human intellectual disability, but the cellular etiology of this association is unknown. We identify the neuronal WASH complex proteome, revealing a network of endosomal proteins. To…

A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus

E. Keats Shwab; Praveen R. Juvvadi; Greg Waitt dkk.

mBio, Vol. 8 No. 1 · 2017 · Inggris · DOAJ Scopus

ABSTRACT Invasive aspergillosis (IA), caused by the filamentous fungal pathogen Aspergillus fumigatus, is a major cause of death among immunocompromised patients. The cyclic AMP/protein kinase A (PKA) signaling pathway is essential for hyphal growth and virulence of A. fumigatus, but the mechanism…