150 results for "Roger Stephan"
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Knife Decontamination by Cold Water Treatment Supplemented with InspexxTM 210—A Validation Study in an Abattoir
To evaluate the decontamination effect of Inspexx 210, a cold-water disinfectant containing peracetic acid and peroctanoic acid (0.16%), knives contaminated with E. coli ATCC 25922 and S. aureus ATCC 29213 as well as naturally contaminated knives collected from different slaughtering steps of an…
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Pregnancy in Slaughtered Lambs and Sheep—A Cross-Sectional Study in Three Abattoirs in Switzerland
The slaughter of pregnant sheep and goats is not restricted in Switzerland. The aim of this study was to assess the prevalence of pregnant sheep and lambs being slaughtered in Switzerland and to determine the state of gestation and vital signs of the fetuses in order to assess the need to take…
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Relevant Aspects of Clostridium estertheticum as a Specific Spoilage Organism of Vacuum-Packed Meat
Clostridium estertheticum is a psychrotolerant, gram-positive, motile, anaerobic, spore-forming, rod-shaped bacteria that causes blown pack spoilage (BPS). Spoilage occurs in vacuum-packed meat without temperature abuse. Having been reported in the last 30 years in several countries, BPS by Cl…
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Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine
Abstract A novel TaqMan 5‐plex real‐time PCR using a combination of locked nucleic acid‐modified (LNA)‐ and minor groove binding (MGB)‐conjugated DNA probes was developed for identification and differentiation between the four main pathogenic Brachyspira species in swine. B. hyodysenteriae, B…
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Systematic Literature Review to Determine Existing Data on the Growth of Listeria monocytogenes in Ready-to-Eat Foods Performed Based on the European Union Reference Laboratory (EURL) Lm Technical Guidance Documents
With rising incidence in recent years, Listeriosis, a severe foodborne disease in humans primarily transmitted through ready-to-eat (RTE) foods contaminated with Listeria monocytogenes, became the most severe zoonotic disease in the European Union (EU) in 2024 with the highest hospitalization and…
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A Comprehensive Methodology for Microbial Strain Typing Using Fourier-Transform Infrared Spectroscopy
Timely and accurate detection and characterization of microbial threats is crucial for effective infection and outbreak management. Additionally, in food production, rapid microbe identification is indispensable for maintaining quality control and hygiene standards. Current methods for typing…
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Strain diversity in Mycobacterium avium subsp. paratuberculosis-positive bovine fecal samples collected in Switzerland
Paratuberculosis or Johne’s disease is a chronic intestinal disease in domestic and wild ruminants. It affects global dairy economy and is caused by Mycobacterium avium subsp. paratuberculosis (MAP). The objective of this study was to analyze strain diversity in MAP-positive fecal samples by using…
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A Cross-Sectional Survey of Foodborne Pathogens in Farm-Sold Frozen Dessert in Switzerland
Reports in the literature indicate that ice cream has been associated with illnesses linked to several pathogens, including Listeria monocytogenes. Farm-produced ice cream and sorbet are commonly marketed in Switzerland, yet data on their bacteriological quality are scarce. This study assessed the…
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Analysis of a poultry slaughter process: Influence of process stages on the microbiological contamination of broiler carcasses
In a large-scale Swiss poultry abattoir, a microbiological process analysis of broiler carcasses was performed. At each selected process stage (scalding, plucking, evisceration, washing, and chilling), 90 carcasses from 30 flocks were sampled and examined for Campylobacter, Salmonella, Escherichia…
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Toxin genes and cytotoxicity levels detected in Bacillus cereus isolates collected from cooked food products delivered by Swiss Army catering facilities
Heated food is known to be often contaminated with B. cereus, leading to cases of diarrhoeal or emetic diseases. Battalion kitchens or army catering facilities present a food safety risk, as temperature abuse and long storage time can result in serious public health problems affecting a high number…
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Microbiological Quality of Plant-based Meat-alternative Products Collected at Retail Level in Switzerland
As the interest in plant-based alternative food products has increased significantly in the last years, it is also important to investigate these products regarding microbiological aspects. The aim of this study was to assess the microbiological quality and the occurrence of selected foodborne…
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β-Phenylethylamine as a Natural Food Additive Shows Antimicrobial Activity against Listeria monocytogenes on Ready-to-Eat Foods
Listeria monocytogenes is an important foodborne pathogen and a major cause of death associated with bacterial foodborne infections. Control of L. monocytogenes on most ready-to-eat (RTE) foods remains a challenge. The potential use of β-phenylethylamine (PEA) as an organic antimicrobial against L…
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Listeria monocytogenes Cold Shock Proteins: Small Proteins with A Huge Impact
Listeria monocytogenes has evolved an extensive array of mechanisms for coping with stress and adapting to changing environmental conditions, ensuring its virulence phenotype expression. For this reason, L. monocytogenes has been identified as a significant food safety and public health concern…
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Decontamination of knives used in a slaughterhouse by a commercial non-thermal UV-C treatment
To assess the antimicrobial effect of a commercial UV-C system, knives inoculated with Escherichia coli and Staphylococcus aureus as well as naturally contaminated and collected from the wet and clean area of a slaughterhouse knives were examined. For inoculated knives, UVC treatment for 30 s…
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Cold Shock Proteins Promote Nisin Tolerance in Listeria monocytogenes Through Modulation of Cell Envelope Modification Responses
Listeria monocytogenes continues to be a food safety challenge owing to its stress tolerance and virulence traits. Several listeriosis outbreaks have been linked to the consumption of contaminated ready-to-eat food products. Numerous interventions, including nisin application, are presently…
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Growth potential of Listeria monocytogenes in six different RTE fruit products: impact of food matrix, storage temperature and shelf life
We tested the growth potential of Listeria monocytogenes on six RTE fruit products at low (4°C at the factory followed by 8°C retail/home storage) and abusive (4°C followed by 12°C) storage temperatures. Sliced coconut and fresh cut cantaloupe, as well as a fruit mix containing diced pineapple…
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Occurrence of Salmonella and presumptive Bacillus cereus in sesame products from Swiss retail stores
Sesame products such as tahini (tahin) or halva (halwa or helva), originating from Arabic cuisine, are becoming increasingly popular in Switzerland. Pathogens, such as Salmonella, can contaminate sesame products, as evidenced by various product recalls. In this study, the occurrence of Salmonella…
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Occurrence of Selected Foodborne Pathogens on Frozen Berries Collected at Retail Level in Switzerland
Berries are globally appreciated for their health benefits, particularly due to antioxidants, and have grown in popularity over recent decades. However, several foodborne outbreaks—mainly viral, caused by norovirus and hepatitis A—have been linked to both fresh and frozen berries. In contrast, the…
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Tandem solubility enhancing tags enable the heterologous expression and in vitro maturation of a class IIa bacteriocin, clesteriocin a, identified in Candidatus Clostridium mucoides CM038
The Clostridium estertheticum complex (CEC) consists of closely related bacterial species that are mostly isolated from meat processing environment. Genome mining studies have recently established CEC as a source of class I bacteriocins. However, up until now, class II bacteriocins have not been…
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Fourier transform infrared spectroscopy enables rapid strain typing and cluster analysis of Listeria monocytogenes under diverse growth conditions
Fourier-transform infrared (FTIR) spectroscopy offers a rapid, high-throughput method for microbial strain typing, with potential applications in food safety and outbreak investigations. We evaluated its discriminatory power for typing Listeria monocytogenes using 118 strains representing diverse…